Detection of Blotted Proteins: Methods and Protocols Softcover reprint of the original 1st ed. 2015 [Pehme köide]
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Märksõnad:
Western Blotting: Origin and Ascent of the Species.- Methods to
Concentrate Proteins for Protein Isolation, Proteomic and Peptidomic
Evaluation.- Measuring Protein Concentration on Nitrocellulose and After the
Electrophoretic Transfer of Protein to Nitrocellulose.- Detection of Blotted
Proteins: Not all Blockers are Created Equal.- Protein Stains to Detect
Antigen on Membranes.- Fluorescent Labeling of Proteins and Its Application
to SDS-PAGE and Western Blotting.- Rapid, Antibody-free Detection of
Recombinant Proteins on Blots Using Enzyme Fragment Complementation.- Use of
Non-Radioactive Detection Method for North- and South-Western Blot.-
Immunoblotting using Radiolabeled Reagents for Detection.- Immunoblotting of
Antigens: Whole, Strip and New-Line Nitrocellulose Membrane Immunoblotting
Using the Chemiluminescence Technique.- Detection of Protein Carbonyls by
Means of Biotin Hydrazide Streptavidin Affinity Methods.- Direct
Immunodetection of Antigens within the Precast Polyacrylamide Gel.-
Quantitative Analysis of Signal Transduction with In-Cell Western
Immunofluorescence Assays.- Ultrasensitive Protein Detection on Dot Blots and
Western Blots with Semiconducting Polymer Dots.- Co-detection of Target and
Total Protein by CyDye Labeling and Fluorescent ECL-plex Immunoblotting in a
Standard Proteomics Workflow.- Using Biotinylated Proteins to Demonstrate
Immunodetection of Antigens via Western Blotting, Dot blots and
Immunohistochemistry.- Calcium Binding by Ro 60 Multiple Antigenic Peptides
on PVDF Membrane.- Sequential use of Immunoblots for Characterization of
Autoantibody Specificities.- Nanogold Immunodetection DetectionSystems for
the Identification of Autoantigens by Western Blotting.- Application of
Intermittent Microwave Irradiation to Western Blot Analysis.- Visualization
of Unstained Protein Bands on PVDF.- Multiplexed Fluorescent Immunodetection
Using Low Auto-Fluorescence Immobilon®-FL Membrane.- Cold Microwave-Enabled
Protein Detection and Quantification.- TLC-Blot (Far-Eastern Blot) and Its
Application to Functional Lipidomics.- Analysis of Electroblotted Proteins by
Mass Spectrometry.- On Membrane Renaturation of Recombinant Ro60 Autoantigen
by Calcium Ions.- Phosphoprotein Detection on Protein Electroblot Using a
Phosphate-Specific Fluorophore.- Purification of Tryptic Digests on
Polyvinylidene Difluoride Membrane.- Detection of Blotted Proteins on
Nitrocellulose/PVDF Membranes by Alta.- Non-stripping Rainbow and Multiple
Antigen Detection (MAD) Western Blotting. - Supported Molecular Matrix
Electrophoresis.- Parafilm-M®, an Available Cost Effective Alternative for
Immuno-blot Pouches.- Succinylation-Alcian Blue Staining of Mucins on
Polyvinylidene Difluoride Membranes.- Comparison of Chemiluminescence vs.
Infrared Techniques for Detection of Fetuin-A in Saliva.- A Novel Methodology
for Stripping and Reprobing of Western Blots Originally Developed with
Colorimetric Substrate TMB.- Other Notable Methods of Membrane Protein
Detection: A Brief Review.- Nitrocellulose Membrane: The New Canvas.-
Invisible Ink Marking in ECL Membrane Assays.
Concentrate Proteins for Protein Isolation, Proteomic and Peptidomic
Evaluation.- Measuring Protein Concentration on Nitrocellulose and After the
Electrophoretic Transfer of Protein to Nitrocellulose.- Detection of Blotted
Proteins: Not all Blockers are Created Equal.- Protein Stains to Detect
Antigen on Membranes.- Fluorescent Labeling of Proteins and Its Application
to SDS-PAGE and Western Blotting.- Rapid, Antibody-free Detection of
Recombinant Proteins on Blots Using Enzyme Fragment Complementation.- Use of
Non-Radioactive Detection Method for North- and South-Western Blot.-
Immunoblotting using Radiolabeled Reagents for Detection.- Immunoblotting of
Antigens: Whole, Strip and New-Line Nitrocellulose Membrane Immunoblotting
Using the Chemiluminescence Technique.- Detection of Protein Carbonyls by
Means of Biotin Hydrazide Streptavidin Affinity Methods.- Direct
Immunodetection of Antigens within the Precast Polyacrylamide Gel.-
Quantitative Analysis of Signal Transduction with In-Cell Western
Immunofluorescence Assays.- Ultrasensitive Protein Detection on Dot Blots and
Western Blots with Semiconducting Polymer Dots.- Co-detection of Target and
Total Protein by CyDye Labeling and Fluorescent ECL-plex Immunoblotting in a
Standard Proteomics Workflow.- Using Biotinylated Proteins to Demonstrate
Immunodetection of Antigens via Western Blotting, Dot blots and
Immunohistochemistry.- Calcium Binding by Ro 60 Multiple Antigenic Peptides
on PVDF Membrane.- Sequential use of Immunoblots for Characterization of
Autoantibody Specificities.- Nanogold Immunodetection DetectionSystems for
the Identification of Autoantigens by Western Blotting.- Application of
Intermittent Microwave Irradiation to Western Blot Analysis.- Visualization
of Unstained Protein Bands on PVDF.- Multiplexed Fluorescent Immunodetection
Using Low Auto-Fluorescence Immobilon®-FL Membrane.- Cold Microwave-Enabled
Protein Detection and Quantification.- TLC-Blot (Far-Eastern Blot) and Its
Application to Functional Lipidomics.- Analysis of Electroblotted Proteins by
Mass Spectrometry.- On Membrane Renaturation of Recombinant Ro60 Autoantigen
by Calcium Ions.- Phosphoprotein Detection on Protein Electroblot Using a
Phosphate-Specific Fluorophore.- Purification of Tryptic Digests on
Polyvinylidene Difluoride Membrane.- Detection of Blotted Proteins on
Nitrocellulose/PVDF Membranes by Alta.- Non-stripping Rainbow and Multiple
Antigen Detection (MAD) Western Blotting. - Supported Molecular Matrix
Electrophoresis.- Parafilm-M®, an Available Cost Effective Alternative for
Immuno-blot Pouches.- Succinylation-Alcian Blue Staining of Mucins on
Polyvinylidene Difluoride Membranes.- Comparison of Chemiluminescence vs.
Infrared Techniques for Detection of Fetuin-A in Saliva.- A Novel Methodology
for Stripping and Reprobing of Western Blots Originally Developed with
Colorimetric Substrate TMB.- Other Notable Methods of Membrane Protein
Detection: A Brief Review.- Nitrocellulose Membrane: The New Canvas.-
Invisible Ink Marking in ECL Membrane Assays.