Cytokine profiling of tumor-infiltrating T lymphocytes by flow cytometry Jeremy B. Foote, Sujith Sarvesh and Leisha A. Emens High-throughput identification of human antigen-specific CD8+ and CD4+ T cells using soluble pMHC multimers Morgane Magnin, Philippe Guillaume, George Coukos, Alexandre Harari and Julien Schmidt In vitro assays for effector T cell functions and activity of immunomodulatory antibodies Roberta Zappasodi, Sadna Budhu, Mohsen Abu-Akeel and Taha Merghoub A cytofluorimetric assay to evaluate T cell polyfunctionality Belinda Palermo, Mariangela Panetta, Giulia Campo and Paola Nistico Assessment of memory formation by metabolically engineered antigen-specific CD8 T cells Mathias Wenes, Pedro Romero and Lianjun Zhang Functional characterization of tumor antigen-specific T-cells isolated from the tumor microenvironment of sleeping beauty induced murine glioma models Mahmoud S. Alghamri, Felipe J. Nunez, Neha Kamran, Stephen Carney, David Altshuler, Pedro R. Lowenstein and Maria G. Castro Methods to edit T cells for cancer immunotherapy Francesca Lucibello, Silvia Menegatti and Laurie Menger Generating stem-like memory T cells with antioxidants for adoptive cell transfer immunotherapy of cancer Karolina Pilipow, Eloise Scamardella and Enrico Lugli Reverse immunology: From peptide sequence to tumor-killing human T-cell clones Christophe Vanhaver, Monica Gordon-Alonso, Alexandre Bayard, Maria Teresa Catanese, Didier Colau, Pierre van der Bruggen and Annika M. Bruger Generation of TCR-engineered reference cell samples to control T-cell assay performance Nicole Bidmon, Cecile Gouttefangeas and Sjoerd H. van der Burg In vitro expansion of V 9V 2 T cells for immunotherapy Christian Peters, Leonce Kouakanou, Hans-Heinrich Oberg, Daniela Wesch and Dieter Kabelitz CFSE dilution to study human T and NK cell proliferation in vitro Inigo Terren, Ane Orrantia, Joana Vitalle, Olatz Zenarruzabeitia and Francisco Borrego Rapid isolation and enrichment of mouse NK cells for experimental purposes Maite Alvarez, Maria C. Ochoa, Luna Minute, Ignacio Melero and Pedro Berraondo Assessment of NK cell-mediated cytotoxicity by flow cytometry after rapid, high-yield isolation from peripheral blood Peter Holicek, Iva Truxova, Lenka Kasikova, Sarka Vosahlikova, Cyril Salek, Jana Rakova, Monika Holubova, Daniel Lysak, Isabelle Cremer, Radek Spisek and Jitka Fucikova Two-dimensional dynamic evaluation of natural killer cell-mediated lysis of adherent target cells Alexandra Frazao, Louise Rethacker and Anne Caignard Rapid isolation of mouse ILCs from murine intestinal tissues Kyle Burrows, Pailin Chiaranunt, Louis Ngai and Arthur Mortha Detecting and analyzing murine innate lymphoid cells Alejandra Gomez-Cadena, Pedro Romero, Sara Trabanelli and Camilla Jandus A cytofluorimetric assay to evaluate intracellular cytokine production by NK cells Christian Sordo-Bahamonde, Seila Lorenzo-Herrero, Segundo Gonzalez and Alejandro Lopez-Soto Applications of microfluidic devices in advancing NK-cell migration studies Xiaoou Ren, Abdulaziz Alamri, Jolly Hipolito, Francis Lin and Sam K.P. Kung Complementary approaches to study NKT cells in cancer Jay A. Berzofsky, Purevdorj B. Olkhanud and Masaki Terabe Assessment of IFN- and granzyme-B production by in "sitro" technology Claudia Galassi, Gwenola Manic, Martina Musella, Antonella Sistigu and Ilio Vitale Assessment of IFN responsiveness in patient-derived xenografts Jordan J. Cardenas, Camila Robles-Oteiza and Katerina Politi Real-time cell analysis (RTCA) to measure killer cell activity against adherent tumor cells in vitro Hans-Heinrich Oberg, Christian Peters, Dieter Kabelitz and Daniela Wesch Analysis of cancer cell-intrinsic immune regulation in response to CD8+ T cell attack Natalie J. Neubert, Laure Tille, Christophe Martignier, Silvia A. Fuertes Marraco and Daniel E. Speiser A flow cytometry-based method to screen for modulators of tumor-specific T cell cytotoxicity Javier Santos, Jesus Ogando, Rosa Ana Lacalle and Santos Manes Evaluation of NK cell cytotoxic activity against malignant cells by the calcein assay Seila Lorenzo-Herrero, Christian Sordo-Bahamonde, Segundo Gonzalez and Alejandro Lopez-Soto 51Cr-release to monitor NK cell cytotoxicity Leslie Elsner and Ralf Dressel
Lorenzo Galluzzi (born 1980) is currently Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College (New York, USA), and Honorary Associate Professor at the Faculty of Medicine of the Paris Descartes University (Paris, France). Prior to joining Weill Cornell Medical College (2017), Lorenzo Galluzzi was a Junior Scientist of the Research Team "Apoptosis, Cancer and Immunity" at the Cordeliers Research Center (Paris, France; 2012-2016). Lorenzo Galluzzi did his post-doctoral training at the Gustave Roussy Cancer Center (Villejuif, France; 2009-2011), after receiving his PhD from the Paris Sud University (Le Kremlin-Bicetre, France; 2005-2008). He is also Associate Director of the European Academy for Tumor Immunology (EATI), and Founding Member of the European Research Institute for Integrated Cellular Pathology (ERI-ICP). Lorenzo Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. In particular, he provided profound insights into the links between adaptive stress responses in cancer cells and the activation of a clinically relevant tumor-targeting immune response in the context of chemotherapy and radiation therapy. Lorenzo Galluzzi has published more than 350 scientific articles in international peer-reviewed journals. According to a survey published by Lab Times, he is currently the 6th and the youngest of the 30 most-cited European cell biologists (relative to the period 2007-2013). Lorenzo Galluzzi currently operates as Editor-in-Chief of three journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, and Molecular and Cellular Oncology (which he co-founded in 2013). In addition, Lorenzo Galluzzi currently serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease. Nils-Petter Rudqvist received his M.Sc. (Physics, 2009) and Ph.D. (Medical Science, 2015) from the University of Gothenburg, Sweden. He decided to pursue an academic career and continue with his postdoctoral training in US. He first joined the Center for Radiological Research at Columbia University in New York where he studied gene signatures of radiation exposure. He then moved to Weill Cornell Medicine to join the program in radiation and immunity under the mentorship of Dr. Demaria. His current research is focused on investigating which neoantigens are key targets of the radiation-induced anti-tumor T cell response in mice and in patients treated with radiotherapy and immune checkpoint blockade. He recently demonstrated in a mouse model that radiation therapy diversifies the TCR repertoire of tumor infiltrating lymphocytes, an effect crucial for its synergy with immune checkpoint blockade treatment. Nils-Petter has also defined unique patters of expansion of TCR clonotypes in patients who respond or not to treatment with radiotherapy and ipilimumab. He has published 20 articles in peer-reviewed scientific journals and 60+ scientific conference abstracts.