Non-Natural Nucleic Acids: Methods and Protocols 1st ed. 2019 [Kõva köide]

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  • Formaat: Hardback, 315 pages, kõrgus x laius: 254x178 mm, kaal: 843 g, 41 Illustrations, color; 54 Illustrations, black and white; XI, 315 p. 95 illus., 41 illus. in color., 1 Hardback
  • Sari: Methods in Molecular Biology 1973
  • Ilmumisaeg: 24-Apr-2019
  • Kirjastus: Humana Press Inc.
  • ISBN-10: 1493992155
  • ISBN-13: 9781493992157
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  • Formaat: Hardback, 315 pages, kõrgus x laius: 254x178 mm, kaal: 843 g, 41 Illustrations, color; 54 Illustrations, black and white; XI, 315 p. 95 illus., 41 illus. in color., 1 Hardback
  • Sari: Methods in Molecular Biology 1973
  • Ilmumisaeg: 24-Apr-2019
  • Kirjastus: Humana Press Inc.
  • ISBN-10: 1493992155
  • ISBN-13: 9781493992157
Teised raamatud teemal:
This volume provides relevant synthetic strategies, incorporation, and applications of non-natural nucleic acids. Chapters detail monomer synthesis, oligomer synthesis/construction, and applications allowing researchers to explore and determine which methodology or methodologies are relevant to their needs.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Non-Natural Nucleic Acids: Methods and Protocols aims to serve as a guide for researchers exploring their own inquiries and to provide a springboard for new endeavors.
1. Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs Stella Diafa, Damien Evequoz, Christian J. Leumann, and Marcel Hollenstein
2. Synthesis of Site-Specific Crown Ether Adducts to DNA Abasic Sites, 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine and 2'-Deoxycytidine Na An, Aaron M. Fleming, Nicole C. Rosecrans, Yi Liao, and Cynthia J. Burrows
3. Synthesis of a Fluorescent Cytidine TNA Triphosphate Analogue Hui Mei and John Chaput
4. Synthesis of Base-Modified dNTPs through Cross-Coupling Reactions and Their Polymerase Incorporation to DNA Petra Menova, Hana Cahova, Milan Vrabel, and Michal Hocek
5. 2'-C,4'-C-Ethyleneoxy-Bridged 2'-Deoxyribonucleic Acids (EoDNAs) with Thymine Nucleobases: Synthesis, Duplex-Forming Ability, and Enzymatic Stability Takashi Osawa, Satoshi Obika, and Yoshiyuki Hari
6. Synthesis Protocols for Simple Uncharged Glycol Carbamate Nucleic Acids Tanaya Bose and Vaijayanti A. Kumar
7. Synthesis of Nucleobase-Functionalized Morpholino Monomers Bappaditya Nandi, Sankha Pattanayak, Sibasish Paul, Jayanta Kundu, and Surajit Sinha
8. Synthesis and Application of LK T Peptide Nucleic Acids Nathaniel Shank, Kara M. George Rosenker, Ethan A. Englund, Andrew V. Dix, Elizabeth E. Rastede, and Daniel H. Appella
9. Aminoglycoside Functionalization as a Tool for Targeting Nucleic Acids Derrick Watkins, Krishnagopal Maiti, and Dev P. Arya
10. Preparation and Purification of Oligodeoxynucleotide Duplexes Containing a Site-Specific, Reduced, Chemically-Stable Covalent Interstrand Cross-Link between a Guanine Residue and an Abasic Site Maryam Imani Nejad, Xu Guo, Kurt Housh, Christopher Nel, Zhiyu Yang, Nathan E. Price, Yinsheng Wang, and Kent S. Gates
11. Copper-Catalyzed Alkyne-Azide Cycloaddition on the Solid Phase for the Preparation of Fully Click-Modified Nucleic Acids Malte Rosenthal, Franziska Pfeiffer, and Gunter Mayer
12. Labeling Peptide Nucleic Acids with Indium-111 Igor G. Panyutin
13. Site-Specific Labeling of DNA via PCR with an Expanded Genetic Alphabet Michael P. Ledbetter, Denis A. Malyshev, and Floyd E. Romesberg
14. Flexible Nucleic Acids (FNAs) As Informational Molecules: Enzymatic Polymerization of fNTPs on DNA Templates and Nonenzymatic Oligomerization of RNA on FNA Templates Maryline Chemama and Christopher Switzer
15. Artificial Nucleosides as Diagnostic Probes to Measure Translesion DNA Synthesis Jung-Suk Choi and Anthony Berdis
16. FRET Assay for Ligands Targeting the Bacterial A-Site RNA Renatus W. Sinkeldam and Yitzhak Tor
17. The Use of Serinol Nucleic Acids as Ultrasensitive Molecular Beacons Keiji Murayama, Hiromu Kashida, and Hiroyuki Asanuma
18. Oligonucleotide Primers with G8AE-Clamp Modifications for RT-qPCR Detection of the Low-Copy dsRNA Timofei S. Zatsepin, Anna M. Varizhuk, Vladimir G. Dedkov, German A. Shipulin, and Andrey V. Aralov
19. Determining Steady-State Kinetics of DNA Polymerase Nucleotide Incorporation Hailey L. Gahlon and Shana J. Sturla

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