RNA Chaperones: Methods and Protocols Second Edition 2026 [Kõva köide]

A two-step purification strategy for RNA and RNA-Protein complexes for
crystallization.- Analysis of tRNA aminoacylation by northern blotting and
multiplex small RNA-Seq.- Characterization of RNA Structure Dynamics with
trans-factors using Single-Molecule Optical Tweezers.- Specific nucleic acid
chaperone activity of HIV-1 nucleocapsid protein deduced from hairpin
unfolding.- A dual-color electrophoretic mobility shift assay to examine RNA
binding and RNA chaperone activity simultaneously.- Use of tRNA mediated
suppression to assess RNA chaperone function.- Salt-dependent modulation of
the RNA chaperone activity of RNA-binding protein La.- Kinetic and
thermodynamic analyses of RNAprotein interactions.- Detection of MicroRNAs
released from Argonautes.- Mapping the RNA chaperone activity of the T.
brucei editosome using SHAPE-chemical probing.- Using immunoprecipitation
followed by dot blotting to identify oligonucleotide-protein interactions in
cells.- In vivo RNA chemical footprinting analysis in Archaea.- RNA structure
analysis by chemical probing with DMS and CMCT.- Prediction of intrinsically
disordered regions that bind RNA with DisoRDPbind.- Delivering Molecular
Beacons via an Electroporation-Based Approach Enables Live-Cell Imaging of
Single RNA Transcripts and Genomic Loci.- Site-specific dual-color labeling
of long RNAs.- Characterizing complex nucleic acid interactions of LINE1
ORF1p by single molecule force spectroscopy.- Isolation and analysis of
bacterial ribosomes through sucrose gradient ultracentrifugation.