Enzyme Catalysis and Steady-State Kinetics: Historical Introduction, Theories of Enzyme Catalysis, and Some Elementary Considerations of Enzyme Kinetics. A Description of Steady-State Kinetics and Quasi- or Rapid Equilibrium Kinetics by a Development of the Rate Expressions for Several Selected Mechanisms and Their Characteristics. Inhibition and Product Inhibition. Effects of pH and Temperature. Effect of Metal Cofactors on the Reaction Velocity. Enzyme Kinetics and Substrate Binding: Some Remarks on Isotope Exchange Studies and Kinetic Isotope Effects. Non-Michaelis-Menten Kinetics and Allosteric Kinetics. Equilibrium Ligand Binding-Multiple Equilibria. Some Complex Kinetic Mechanisms and Treatment of Enzyme Kinetic Data. Kinetics of the Transient Phase or Pre-Steady-State Phase of Enzyme Reactions. Appendix
2. References. Index.
Stephen A. Kuby, Ph.D., is a Professor of Biochemistry and Research Professor of Medicine at the University of Utah School of Medicine, Salt Lake City, Utah. He is also the head of the Biochemical Division of the Laboratory for the Study of Hereditary and Metabolic Disorders, University of Utah Research Park, Salt Lake City, Utah 84108. He graduated SummacumLaude from New York University in 1948 with an A.B. in Chemistry. He obtained his M.S. in Biochemistry in 1951, and his Ph.D. in Biochemistry in 1953 at the University of Wisconsin under Professor H. A. Lardy. As a recipient of the U.S. Public Health Service Fellowship, he spent his post-doctorate period with Professor B. Chance at the University of Pennsylvania (Johnson Foundation for Medical Physics) and with Professor H. Theorell at the Karolinska Institute (Medical Nobel Institute, Stockholm, Sweden). Following a period at the Enzyme Institute (University of Wisconsin) as an Assistant Professor, in 1963 he joined the faculty at the University of Utah, and has held his present positions since 1969. His research interests have dealt with many aspects of enzyme and protein chemistry and certain aspects of medicinal chemistry and inherited disorders (including the muscular dystrophies). Mechanistic enzymology is his current interest, and "state of the art" approaches are being applied to study of enzyme action, e.g., of the kinases.