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E-raamat: Cryptosporidium: Methods and Protocols

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Cryptosporidium: Methods and ProtocolsSuurem pilt
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This second edition volume expands on the previous edition with new chapters discussing the latest protocols that encompass the detection, characterization, and genomic analysis of Cryptosporidium. The first few chapters in this book focus on diagnostic assays, such as traditional microscopy-based techniques, nucleic acid-based molecular detection, and immunoassay-based methodologies. Several chapters cover whole genome sequencing methodologies, genotyping approaches, and novel techniques such as hybridization bait capture analyses. A significant portion of this book is dedicated to functional genomics and experimental models, with discussions on tools used for genetic manipulation (including CRISPR/cas9), gene silencing, and host-pathogen interactions. The volume concludes with chapters exploring drug discovery and testing, highlighting high-content screening methods, phenotypic assays, and efficacy testing of potential disinfectants and therapeutics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Cryptosporidium: Methods and Protocols, Second Edition is a valuable tool for researchers interested in gaining more insight on foundational and innovative Cryptosporidium research, which will inspire future advancements in this field.

Cryptosporidium Diagnostic Assays: Microscopy.- Cryptosporidium
Diagnostic Assays: Molecular Detection and Species Determination by Real-Time
PCR-Based Assays.- Detection of Cryptosporidium Recovered from Large-Volume
Water Samples Using Dead-End Ultrafiltration.- Genomic Characterization of
Cryptosporidium spp. via iNextEra Library Preparation and Hybridization Bait
Capture.- Preparation of Cryptosporidium for Whole Genome Sequencing.- A
Real-Time PCR Based Method for the Sequence Determination of Cryptosporidium
hominis and Cryptosporidium parvum gp60 Subtypes During Epidemiological
Investigations.- Cryptosporidium Genotyping for Epidemiology Tracking.- A
Multi-locus Variable Number Tandem Repeat Analysis (MLVA) Scheme for
Epidemiological Investigations of Cryptosporidium parvum.- Optimized Genome
Assembly Protocol for Cryptosporidium.- Evolutionary Genomic Analyses of
Cryptosporidium.- A Method to Silence Genes in Cryptosporidium.- Use of
ncRNAs to Study Host Cell-Parasite Interactions.- Genetic Manipulation of
Cryptosporidium parvum.- Mouse Models for Use in Cryptosporidium Infection
Studies, Quantification of Parasite Burden Using Flow Cytometry, qPCR and
Histopathology, and Confocal Imaging of Oocysts.- Calf Clinical Model of
Cryptosporidiosis for Efficacy Evaluation of Therapeutics.- Cell Culture
Infectivity to Assess Chlorine Disinfection of Cryptosporidium Oocysts in
Water.- High-Content Screening for Cryptosporidium Drug Discovery.-
Phenotypic High-Throughput Screening and Drug Efficacy Test Against the
Growth of Cryptosporidium parvum In Vitro by qRT-PCR.- Establishing TurboID
Proximity Labeling and mAID Conditional Knockdown System in Cryptosporidium
parvum.- In Vitro Culture of Cryptosporidium spp. Using Stem Cell-Derived
Intestinal Epithelial Monolayers.- Two and Three Dimensional Human Intestinal
Tissue Models for Cryptosporidium Infection.- Cryptosporidium Oocyst
Purification Using Discontinuous Gradient Centrifugation.
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