Immuno-oncology and immunotherapy, Part F, Volume 201 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including an Evaluation of agents that affect anti-tumor function of CD8+ T cells at activation, Cancer cyro-immunotherapy: Models and methods, Methods for analysis of cancer- and TLS-associated immune infiltrates by multiplex immunofluorescence histology, In-vivo measurement of the extracellular ATP concentration by bio-luminescent probes, and much more.
Additional sections cover the Analysis of the tumor microenvironment using imaging mass cytometry data, Saphenous vein blood collection for different immune analyses of living mice, Use of mouse KO models to validate the specificity of monoclonal antibodies, Imaging mass cytometry to analyze the immune TME, Autoimmune arthritis: Transgenic mouse models and methods, Assessment of human and mouse tumor-antigen specific CD8+ T cells by multimer staining in multiple compartments, and more.
1. Generation and functional evaluation of STAb-T cells
Luis Alvarez-Vallina
2. A syngeneic orthotopic mouse model of metastatic colorectal cancer
Christel Devaud
3. High-parameter T-cell spectral phospho flow cytometry
Joshua Brody and Gvantsa Pantsulaia
4. Measuring T-cell/CAR T-cell bystander tumor killing in vitro and in vivo
Joshua Brody, Matthew Lin and Daniel Charytonowicz
5. Quantification and functional assessment of antigen(-) cancer cell
variants
Joshua Brody, Gvantsa Pantsulaia, Ivan Odak and Xie Xinping
6. T-cell and CAR T-cell Cas9-RNP/nucleofection-mediated gene editing
Joshua Brody, Gvantsa Pantsulaia and Moah Sohn
7. Peripheral blood mononuclear cell (PBMC)- based functional evaluation of
human T cell response to suppressive cells and immune-oncology therapeutics
Maria G. Castro, Kaushik Banerjee, Pedro R. Lowenstein, Mahmoud Alghamri,
Brandon McClellan and Jorge Peña Agudelo
8. Integrative analysis of lung multiparametric flow cytometry data to study
immune cell dynamics in tumors
Romina Goldszmid and Romina Araya
9. Multiparametric staining: Combined application of immunofluorescence and
in-situ hybridization
Barbara Seliger, Marcus Bauer, Andreas Wilfer, Clara Zöllig and Claudia
Wickenhauser
10. Extracellular matrix and morphology assessment method on live 3D
spheroids of thyroid carcinoma
Barbara Seliger, Mario Udinotti, Udo Sieboldts and Christoforos Vaxevanis
11. Hema-CRISPR: A clone-based protocol for effective genetic editing of
hematopoietic stem cells
Barbara Seliger, Christoforos Vaxevanis, Mario Udinotti, Dimitris Kokoretsis,
Andreas Wilfer, Marcus Bauer and Claudia Wickenhauser
12. Patient-derived models of tumor-immune cell interactions
Zlatko Trajanoski
13. MDSC isolation from human tumors
Viktor Umansky, Feyza Gul Ozbay Kurt and Jochen Utikal
14. Extracellular vesicles isolation from human maternal fluids
Sheila Spada
15. Finding a Needle in a Haystack: Measures of Circulating Tumor DNA (ctDNA)
to Guide Treatment Decisions in Oncology
Lotze, Michael T, Genia Dubrovsky, Jeff Szymanski, Aadel A. Chaudhuri, Jose
Zevallos, Noah Earland, Peter Harris and Pradeep Chauhan
Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.
Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience. Norma received her masters degree in health biology from Paris Saclay. In 2013, she joined the laboratory of Dr. Guido Kroemer at the Centre de Recherche des Cordeliers (Paris, France) and at Gustave Roussy (Villejuif, France), the largest center for oncological patients in Europe. She worked on several projects linked to immunosurveillance, culminating with her obtaining her PhD in 2017 with an original work on Immunogenic stress and death of cancer cells: Contribution of antigenicity vs adjuvanticity to immunosurveillance”. She then moved to Weill Cornell Medicine to join the program in radiation and immunity under the mentorship of Dr. Galluzzi. Her current research is focused on investigating resistance to immunotherapy in a mouse model of HR+ breast cancer.
Maud Charpentier received her M.Sc. in Cellular Biology and her Ph.D. in Immunology from the University of Nantes, France. She chose to pursue an academic career and continued her postdoctoral training in the United States. She joined the Department of Radiation Oncology at Weill Cornell Medicine under the mentorship of Dr. Sandra Demaria. Maud has a long-standing interest in the anti-tumor immune response and its role in controlling cancer progression and treatment outcomes. Her research focuses on understanding the synergy between radiation therapy and immunotherapy in solid tumors, with the aim of overcoming resistance to treatment and developing innovative therapeutic approaches in preclinical models. Jose Manuel Bravo-San Pedro is currently a researcher at the Department of Physiology of the Complutense University of Madrid thanks to a Ramon y Cajal contract grant. He got his Ph.D. in biochemistry, cellular biology and genetics from the University of Extremadura (Caceres, Spain) in 2011, and he did a post-doctoral stage in the laboratory of Prof. Guido Kroemer. His main research interests have always been linked to autophagy, addressing this cellular process associated with neurodegenerative diseases or cancer and recently obesity and specifically related to problems in the correct functioning of the cilium. He is co-inventor of two patents and co-author of 110 publications indexed in PubMed in prestigious international journals, with h-index 45 and 23768 cites (Dec 2022).
