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E-raamat: Microbial Plant Pathogens - Detection and Management in Seeds and Propagules: Detection and Management in Seeds and Propagules [Wiley Online]

  • Formaat: 1184 pages
  • Ilmumisaeg: 03-Feb-2017
  • Kirjastus: John Wiley & Sons Inc
  • ISBN-10: 1119195802
  • ISBN-13: 9781119195801
  • Wiley Online
  • Hind: 206,17 €*
  • * hind, mis tagab piiramatu üheaegsete kasutajate arvuga ligipääsu piiramatuks ajaks
  • Formaat: 1184 pages
  • Ilmumisaeg: 03-Feb-2017
  • Kirjastus: John Wiley & Sons Inc
  • ISBN-10: 1119195802
  • ISBN-13: 9781119195801
Healthy seeds and propagules are the basic requirement for producing good grains, fruits and vegetables needed for human survival and perpetuation. Dispersal of microbial plant pathogens via seeds and propagules has assumed more importance than other modes of dispersal, as infected seeds and propagules have the potential to become the primary sources of carrying pathogen inoculum for subsequent crops. Several diseases transmitted through seeds and propagules have been shown to have the potential to damage economies as a result of huge quantitative and qualitative losses in numerous crops. Hence, it is essential to rapidly detect, identify and differentiate the microbial plant pathogens present in seeds and propagules precisely and reliably, using sensitive techniques.

Microbial Plant Pathogens: Detection and Management in Seeds and Propagules provides a comprehensive resource on seed-borne and propagule-borne pathogens. Information on the biology of microbial pathogens, including genetic diversity, infection process and survival mechanisms of pathogens and epidemiology of diseases caused by them, are discussed critically and in detail to highlight weak links in the life cycles of the pathogens. Development of effective disease management systems, based on the principles of exclusion and eradication of pathogens and immunization of crop plants to enhance the levels of resistance of cultivars to diseases, has been effective to keep the pathogens at bay. The need for production of disease-free seeds/propagules has been emphasized to prevent the carryover of the inoculum to the next crop or introduction of the pathogens to other locations. Effectiveness of adopting simple cultural practices and development of cultivars resistant to diseases through traditional breeding methods or biotechnological approach have resulted in reducing the pathogen inoculum and disease incidence. Although application of different chemicals may reduce the disease incidence effectively, biological management of crop diseases, employing potential biological control agents have to be preferred to preserve the agroecosystems. Greater efforts have to be made to integrate compatible strategies to enhance the effectiveness of diseases management systems. Protocols appended at the end of relevant chapters form a unique feature of this book to enable the researchers to fine-tune their projects.

This 2 volume set provides comprehensive and updated information about the economically-important groups of microbial plant pathogens carried by seed and propagules. Graduate students, researchers and teachers of plant pathology, plant protection, microbiology, plant breeding and genetics, agriculture and horticulture, as well as certification and quarantine personnel will find the information presented in this book useful.
Volume 1 Pathogen Detection and Identification
Preface
xv
Acknowledgement
xvii
1 Introduction
3(9)
1.1 Concepts and Implications of Pathogen Infection of Seeds and Propagules
3(1)
1.2 Economic Importance of Seed- and Propagule-Borne Microbial Pathogens
4(2)
1.3 Nature of Seed- and Propagule-Borne Microbial Pathogens
6(2)
1.4 Development of Crop Disease Management Systems
8(1)
References
9(3)
2 Detection and Identification of Fungal Pathogens
12(122)
2.1 Detection and Differentiation of Fungal Pathogens in Seeds
12(74)
2.1.1 Conventional/Isolation-Dependent Methods
13(6)
2.1.1.1 Dry Seed Examination
14(1)
2.1.1.2 Histological and Cytological Methods
15(1)
2.1.1.3 Seed Washing Test
16(1)
2.1.1.4 Blotter Test
16(2)
2.1.1.5 Direct Plating
18(1)
2.1.2 Isolation-Independent Methods
19(5)
2.1.2.1 Grow-Out/Seedling Symptom Test
19(1)
2.1.2.2 Physical Methods
20(2)
2.1.2.3 Chemical Methods
22(2)
2.1.3 Immunoassays
24(4)
2.1.4 Nucleic Acid-Based Techniques
28(58)
2.1.4.1 Hybridization-Based Nucleic Acid Techniques
28(1)
2.1.4.2 PCR-Based Techniques
29(30)
2.1.4.3 Combination of PCR-Based Assays with Other Techniques
59(2)
2.1.4.4 Reverse-Transcription (RT) Polymerase Chain Reaction
61(1)
2.1.4.5 Random Amplified Polymorphic DNA Technique
61(6)
2.1.4.6 Amplified Fragment Length Polymorphism
67(3)
2.1.4.7 Restriction Fragment Length Polymorphism
70(5)
2.1.4.8 DNA Sequence Analysis
75(4)
2.1.4.9 Phylogenetic Analysis
79(1)
2.1.4.10 DNA Array Technology
80(1)
2.1.4.11 Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry
81(1)
2.1.4.12 Biochemical Methods
81(3)
2.1.4.13 Assessment of Variations in Biological Characteristics of Fungal Pathogens
84(2)
2.2 Detection and Differentiation of Fungal Pathogens in Propagules
86(18)
2.2.1 Conventional/Isolation-Dependent Methods
87(1)
2.2.2 Isolation-Independent Methods
88(14)
2.2.2.1 Immunoassays
88(2)
2.2.2.2 Nucleic Acid-Based Techniques
90(11)
2.2.2.3 Loop-Mediated Isothermal Amplification
101(1)
2.2.3 Techniques Based on Biological Characteristics of Fungal Pathogens
102(2)
2.2.3.1 Vegetative Compatibility Groups
102(2)
2.3 Appendix
104(8)
2.3.1 Alkaline Blotter Method
104(1)
2.3.2 Detection of Teliospores of Tilletia indica in Seed by Acid Electrolyzed Water
104(1)
2.3.2.1 Preparation of Acid Electrolyzed Water and Sodium Hypochlorite Solutions
104(1)
2.3.2.2 Assessment of Effectiveness of AEW and NaOCl
104(1)
2.3.3 Detection of Sporisorium reilianum and Ustilago maydis by Dot-Blot Hybridization
105(1)
2.3.3.1 Isolation of Smut Pathogens
105(1)
2.3.3.2 Nucleic Acid Hybridization
105(1)
2.3.4 Detection of Phoma ligulicola in Pyrethrum Seed by Polymerase Chain Reaction
106(1)
2.3.4.1 Isolation of the Pathogen
106(1)
2.3.4.2 Extraction of Pathogen DNA
106(1)
2.3.4.3 PCR Amplification
106(1)
2.3.5 Detection of Plasmopara halstedii in Sunflower Seed by Optimized Duplex Real-Time PCR Assay
106(1)
2.3.5.1 Preparation of P. halstedii-Infected Seed Materials
106(1)
2.3.5.2 Optimized Duplex qPCR Assay
107(1)
2.3.6 Detection of Colletotrichum lindemuthianum in Common Bean Seed by PCR Assay
107(1)
2.3.6.1 Extraction of Pathogen DNA
107(1)
2.3.6.2 Seed Powder Preparation
107(1)
2.3.6.3 PCR Amplification
107(1)
2.3.7 Detection of Alternaria brassicae by Conventional and Real-Time PCR Assays in Radish Seeds
108(1)
2.3.7.1 Seed Sample Preparation
108(1)
2.3.7.2 PCR-Based Assays
108(1)
2.3.8 Assessment of Genetic Diversity of Fusarium fujikuroi by UP-PCR Analyses
109(1)
2.3.8.1 Extraction of DNA from Pathogen Mycelium
109(1)
2.3.8.2 UP-PCR Amplification
109(1)
2.3.9 Detection and Quantification of Veriticillium dahliae in Spinach Seed by Real-Time PCR Assay
110(1)
2.3.9.1 Extraction of Pathogen DNA from Infected Spinach Seed
110(1)
2.3.9.2 Quantitative PCR Assay
110(1)
2.3.10 Assessment of Variation Among Isolates of Fusarium graminearum Using Random Amplified Polymorphic DNA (RAPD)
111(1)
2.3.11 Differentiation of Tilletia spp. by PCR-RFLP Technique
111(24)
2.3.11.1 Extraction of Pathogen DNA
111(1)
2.3.11.2 PCR Amplification
111(1)
2.3.11.3 DNA Sequencing and Analysis
112(1)
References
112(22)
3 Biology of Fungal Pathogens
134(40)
3.1 Biological Characteristics
135(9)
3.1.1 Disease Cycles of Fungal Pathogens
135(8)
3.1.1.1 Reproduction of Fungal Pathogens
135(1)
3.1.1.2 Survival and Dispersal of Fungal Pathogens
136(7)
3.1.2 Host Range of Fungal Pathogens
143(1)
3.2 Physiological Characteristics of Fungal Pathogens
144(3)
3.2.1 Variations in Virulence of Fungal Pathogens
144(3)
3.3 Genotypic Characteristics of Fungal Pathogens
147(18)
3.3.1 Pathogenicity of Fungal Pathogens
147(2)
3.3.2 Production of Mycotoxins by Fungal Pathogens
149(8)
3.3.3 Production of Enzymes and Other Compounds by Fungal Pathogens
157(3)
3.3.4 Sensitivity of Fungal Pathogens to Fungicides
160(5)
3.4 Influence of Storage Conditions
165(1)
3.5 Appendix
166(1)
3.5.1 Isolation of Fusarium oxysporum f. sp. vasinfectum from Cotton Seed
166(8)
3.5.1.1 Fusarium Selective Liquid Medium
166(1)
3.5.1.2 Isolation of the Pathogen
166(1)
References
166(8)
4 Process of Infection by Fungal Pathogens
174(46)
4.1 Invasion Paths of Seedborne Fungal Pathogens
174(33)
4.1.1 Direct Infection of Floral Parts
174(16)
4.1.2 Infection Through Inoculum in Soil/Plant Residues
190(7)
4.1.3 Infection Through Inoculum Present in Seed-Bearing Organs
197(4)
4.1.4 Role of Enzymes and Toxins in Pathogenesis
201(6)
4.2 Invasion Paths of Propagule-Borne Fungal Pathogens
207(3)
References
210(10)
5 Detection and Identification of Bacterial and Phytoplasmal Pathogens
220(155)
5.1 Detection and Identification of Bacterial Pathogens
220(53)
5.1.1 Detection of Bacterial Pathogens in Seed
220(2)
5.1.2 Conventional/Isolation-Dependent Methods
222(4)
5.1.2.1 Isolation of Bacterial Pathogens
222(3)
5.1.2.2 Bacteriophage Typing
225(1)
5.1.3 Isolation-Independent Methods
226(3)
5.1.3.1 Seedling Grow-Out Assay
226(1)
5.1.3.2 Biochemical Methods
227(1)
5.1.3.3 Physical Techniques
228(1)
5.1.4 Immunoassays
229(13)
5.1.4.1 Agglutination-/Diffusion-Based Tests
230(1)
5.1.4.2 Immunofluorescence Microscopy
231(1)
5.1.4.3 Enzyme-Linked Immunosorbent Assay
232(5)
5.1.4.4 Immunomagnetic Separation of Bacterial Pathogens
237(1)
5.1.4.5 Immunolabeling and Electron Microscopy
238(2)
5.1.4.6 Flow Cytometry
240(2)
5.1.5 Nucleic Acid-Based Assays
242(31)
5.1.5.1 Nucleic Acid Hybridization Methods
242(1)
5.1.5.2 Restriction Fragment Length Polymorphism
243(1)
5.1.5.3 PCR-Based Assay
244(10)
5.1.5.4 Real-Time Polymerase Chain Reaction Assay
254(7)
5.1.5.5 Integration of PCR with Other Detection Methods
261(9)
5.1.5.6 Assessment of Genetic Diversity of Bacterial Pathogens
270(3)
5.2 Detection of Bacterial Pathogens in Propagules
273(53)
5.2.1 Conventional/Biological Methods
273(2)
5.2.2 Phage Typing
275(1)
5.2.3 Microscopy
276(1)
5.2.4 Physical Techniques
276(2)
5.2.5 Immunoassays
278(4)
5.2.6 Nucleic Acid-Based Techniques
282(44)
5.2.6.1 Nucleic Acid Hybridization Methods
282(1)
5.2.6.2 PCR-Based Methods
283(35)
5.2.6.3 Loop-Mediated Isothermal Amplification Technique
318(3)
5.2.6.4 Cycleave Isothermal and Chimeric Primer-Initiated Amplification of Nucleic Acids
321(1)
5.2.6.5 DNA Array Technology
322(3)
5.2.6.6 Nucleic Acid-Based Amplification Assay
325(1)
5.3 Detection of Phytoplasmal Pathogens
326(17)
5.3.1 Immunoassays
328(2)
5.3.2 Nucleic Acid-Based Techniques
330(13)
5.3.2.1 Hybridization Techniques
330(1)
5.3.2.2 Polymerase Chain Reaction Techniques
330(13)
5.3.2.3 Loop-Mediated Isothermal Amplification Assay
343(1)
5.4 Appendix
343(9)
5.4.1 General/Semiselective Media for Isolation of Bacterial Pathogens from Seed
343(1)
5.4.2 Stem-Printing Technique for Evaluation of Seed Transmission of Pantoea stewartii ssp. stewartii in Corn Seedlings
344(1)
5.4.3 Detection of Acidovorax avenae ssp. citrulli (Aac) by MAb-Captured-Sandwich ELISA (MC-sELISA) in Seed Extracts
344(1)
5.4.3.1 Detection of the Pathogen in Culture
344(1)
5.4.3.2 Detection of the Pathogen in Artificially and Naturally Infested Seeds
345(1)
5.4.4 Detection of Clavibacter michiganensis subsp. michiganensis (Cmm) in Tomato Seeds by Immunomagnetic Separation (IMS) Plating Technique
345(1)
5.4.4.1 Coating of Magnetic Beads
345(1)
5.4.4.2 Standardization of Immunoseparation Technique
345(1)
5.4.5 Detection of Xanthomonas campestris pv. carotae by PCR Assay
346(1)
5.4.5.1 Extraction of Pathogen DNA from Seeds
346(1)
5.4.5.2 Polymerase Chain Reaction (PCR) Assay
346(1)
5.4.6 Detection of Burkholderia glumae in Rice Seeds by Real-Time PCR Assay
346(1)
5.4.6.1 Preparation of Seed Wash
346(1)
5.4.6.2 Real-Time PCR Assay
347(1)
5.4.7 Detection and Quantification of Burkholderia spp. in Rice Seeds by Real-Time PCR Assay
347(1)
5.4.7.1 Isolation of DNA for Real-Time PCR Assay
347(1)
5.4.7.2 Real-Time PCR Assay
347(1)
5.4.7.3 Preparation of Standard Curve
348(1)
5.4.8 Detection of Pseudomonas syringae pv. phaseolicola in Bean Seed Washings by Membrane BIO-PCR Assay
348(1)
5.4.9 Detection of Acidovorax avenae subsp. citrulli in the Seeds of Watermelon by IMS-Real-Time PCR Assay
348(1)
5.4.9.1 Immunomagnetic Separation (IMS)
348(1)
5.4.9.2 Real-Time PCR Assay
349(1)
5.4.10 Detection of Acidovorax avenae subsp. citrulli (Aac) by Direct PCR and Immunocapture (IC-) PCR Assays in Watermelon Seeds
349(1)
5.4.10.1 Selection of Antibodies and Primers Specific to Aac
349(1)
5.4.10.2 Detection of Aac by Standard PCR Format
349(1)
5.4.10.3 Detection of Aac by IC-PCR Assay
349(1)
5.4.11 Detection of "Candidates Liberibacter solanacearum," Causative Agent of Potato Zebra Chip Disease by PCR Assay
350(1)
5.4.11.1 Extraction of DNA from Plant Tissues
350(1)
5.4.11.2 PCR Amplification
350(1)
5.4.12 Detection of Erwinia carotovora subsp. atroseptica (Eca) by Loop-Mediated Isothermal Amplification (LAMP) Assay in Potato
350(1)
5.4.12.1 Preparation of Genomic DNA of the Pathogen
350(1)
5.4.12.2 Preparation of Primers
351(1)
5.4.12.3 LAMP Assay
351(1)
5.4.13 Detection of Flavescence Doree Phytoplasma in Grapevine by Reverse-Transcription PCR Assay
351(24)
5.4.13.1 Extraction of Leaf Sap
351(1)
References
352(23)
6 Biology and Infection Process of Bacterial and Phytoplasmal Pathogens
375(82)
6.1 Biology of Bacterial Pathogens
375(2)
6.1.1 General Characteristics of Bacterial Pathogens
375(2)
6.2 Disease Cycles of Seedborne Bacterial Pathogens
377(32)
6.2.1 Xanthomonas spp.
377(14)
6.2.2 Pseudomonas spp.
391(4)
6.2.3 Burkholderia spp.
395(2)
6.2.4 Acidovorax spp.
397(4)
6.2.5 Clavibacter spp.
401(5)
6.2.6 Curtobacterium spp.
406(1)
6.2.7 Pantoea spp.
407(1)
6.2.8 Xylella sp.
408(1)
6.3 Disease Cycles of Propagule-Borne Bacterial Pathogens
409(20)
6.3.1 Erwinia spp.
410(4)
6.3.2 Clavibacter spp.
414(1)
6.3.3 Ralstonia sp.
414(3)
6.3.4 Xanthomonas spp.
417(4)
6.3.5 Candidatus Liberibacter
421(5)
6.3.6 Streptomyces spp.
426(3)
6.4 Biology of Phytoplasmal Pathogens
429(2)
6.4.1 General Characteristics of Phytoplasmal Pathogens
430(1)
6.5 Disease Cycles of Phytoplasmal Pathogens
431(6)
6.5.1 Apple Proliferation Phytoplasma
434(1)
6.5.2 Lime Witches' Broom Phytoplasma
435(1)
6.5.3 Flavescence Doree (FD) Phytoplasma
436(1)
6.5.4 Palm Lethal Yellowing (LY) Phytoplasma
436(1)
6.6 Appendix
437(1)
6.6.1 Stab-Inoculation of Xanthomonas campestris pv. vitians on Lettuce Plants
437(20)
6.6.1.1 Isolation of the Pathogen
437(1)
6.6.1.2 Determination of Pathogen Location in Lettuce Stems
437(1)
References
437(20)
7 Detection and Identification of Viruses and Viroids
457(162)
7.1 Detection of Viruses in Seeds
457(36)
7.1.1 Plant Virus Taxonomy
458(1)
7.1.2 Biological Methods
458(5)
7.1.2.1 Symptoms Induced by Viruses
458(2)
7.1.2.2 Modes of Transmission of Viruses
460(3)
7.1.3 Immunoassays
463(17)
7.1.3.1 Enzyme-Linked Immunosorbent Assay
464(12)
7.1.3.2 Dot-Immunobinding Assay
476(1)
7.1.3.3 Petridish-Agar Dot-Immunomagnetic Assay
477(1)
7.1.3.4 Fluorescent Antibody Technique
477(1)
7.1.3.5 Microscopy-Based Techniques
478(2)
7.1.4 Nucleic Acid-Based Techniques
480(13)
7.1.4.1 Nucleic Acid Hybridization
480(3)
7.1.4.2 Polymerase Chain Reaction Assays
483(8)
7.1.4.3 Combination of PCR Assay with Other Diagnostic Methods
491(2)
7.2 Detection of Viruses in Propagules
493(79)
7.2.1 Bioindexing Methods
494(1)
7.2.2 Immunoassays
495(22)
7.2.2.1 Enzyme-Linked Immunosorbent Assays
499(10)
7.2.2.2 Tissue-Blot Immunoassay
509(3)
7.2.2.3 Immunoblot Assay
512(2)
7.2.2.4 Dot-Immunobinding Assay
514(1)
7.2.2.5 In Situ Immunoassay
515(1)
7.2.2.6 Immunosorbent Electron Microscopy
516(1)
7.2.3 Nucleic Acid-Based Techniques
517(39)
7.2.3.1 Nucleic Acid Hybridization
518(3)
7.2.3.2 Polymerase Chain Reaction Techniques
521(29)
7.2.3.3 Combination of RT-PCR with Other Diagnostic Assays
550(6)
7.2.4 Real-Time PCR Assay
556(6)
7.2.5 Molecular Beacon
562(2)
7.2.6 Single-Strand Conformation Polymorphism Analysis
564(1)
7.2.7 Reverse-Transcription Loop-Mediated Isothermal Amplification
565(3)
7.2.8 DNA Array Technology
568(2)
7.2.9 Small-RNA Deep-Sequencing Analysis
570(2)
7.3 Detection of Viroids in Seeds
572(5)
7.3.1 Classification of Viroids
572(1)
7.3.2 Biological Methods
573(1)
7.3.3 Physico-Chemical Methods
574(1)
7.3.4 Nucleic Acid-Based Techniques
575(2)
7.4 Detection of Viroids in Propagules
577(13)
7.4.1 Nucleic Acid-Based Techniques
577(13)
7.4.1.1 Nucleic Acid Hybridization
578(3)
7.4.1.2 Reverse-Transcription (RT-) PCR Techniques
581(7)
7.4.1.3 Reverse-Transcription Loop-Mediated Isothermal Amplification
588(2)
7.4.1.4 Single-Strand Conformation Polymorphism Analysis
590(1)
7.5 Appendix
590(4)
7.5.1 Detection of Citrus Tristeza Virus by Improved Direct Tissue-Blot Immunoassay (I-DTBIA) in Citrus Plants
590(1)
7.5.1.1 Preparation of Antibodies
590(1)
7.5.1.2 Improved DTBIA Procedure
591(1)
7.5.2 Detection of Tomato Spotted Wilt Virus (TSWV) in Ranunculus Tubers by Tissue-Blot Immunoassay
591(1)
7.5.2.1 Preparation of Plant Tissue Samples
591(1)
7.5.2.2 Tissue-Blot Immunoassay (TBIA)
591(1)
7.5.3 Detection of Citrus Tristeza Virus (CTV) by Hybridization Assays
592(1)
7.5.3.1 Preparation of Nucleic Acid Extracts
592(1)
7.5.3.2 Hybridization
592(1)
7.5.4 Detection of Plum Pox Virus (PPV) by Real-Time RT-PCR and Its Variants
592(1)
7.5.4.1 Preparation of Crude Extracts of Plant Tissues
592(1)
7.5.4.2 Real-Time RT-PCR and Dilution, Spot, Tissue Print, and Squash Variants
593(1)
7.5.5 Detection of Potato Spindle Tuber Viroid by Reverse-Transcription Loop-Mediated Isothermal Amplification (LAMP)
593(27)
7.5.5.1 Extraction of Total Nucleic Acid from Potato Leaf, Tuber, and Tomato Leaf Tissues
593(1)
7.5.5.2 RT-LAMP Reaction
594(1)
References
594(25)
8 Biology and Infection Process of Viruses and Viroids
619(50)
8.1 Characteristics of Plant Viruses
619(1)
8.2 Biological Properties of Viruses
620(12)
8.2.1 Types of Symptoms Induced by Viruses
620(3)
8.2.2 Host Range of Viruses
623(1)
8.2.3 Genetic Variability of Viruses
623(4)
8.2.4 Modes of Transmission of Viruses
627(1)
8.2.5 Cross-Protection of Viruses
628(4)
8.3 Infection Process of Plant Viruses
632(14)
8.3.1 Virus Replication
632(2)
8.3.2 Movement of Plant Viruses
634(6)
8.3.2.1 Cell-to-Cell Movement
634(3)
8.3.2.2 Long-Distance Movement of Viruses
637(3)
8.3.3 Colonization of Plant Tissues by Viruses
640(6)
8.4 Characteristics of Viroids
646(5)
8.4.1 Biological Properties of Viroids
647
8.4.1.1 Types of Symptoms
647(1)
8.4.1.2 Diagnostic Hosts and Host Range
647(2)
8.4.1.3 Modes of Viroid Transmission
649(2)
8.5 Infection Process of Viroids
651(5)
References
656(13)
Index
669
Volume 2 Epidemiology and Management of Crop Diseases
Preface
xi
Acknowledgement
xiii
9 Epidemiology of Seed- and Propagule-Borne Diseases
3(49)
9.1 Epidemiology of Fungal Diseases
4(23)
9.1.1 Dynamics of Host-Fungal-Pathogen Interactions
4(20)
9.1.1.1 Pathogen Factors
5(8)
9.1.1.2 Host Factors
13(3)
9.1.1.3 Environmental Factors
16(8)
9.1.2 Molecular Ecology and Epidemiology of Fungal Diseases
24(3)
9.2 Epidemiology of Bacterial Diseases
27(10)
9.2.1 Dynamics of Host-Bacterial-Pathogen Interactions
27(8)
9.2.1.1 Pathogen Factors
27(5)
9.2.1.2 Host Factors
32(2)
9.2.1.3 Environmental Factors
34(1)
9.2.2 Molecular Ecology and Epidemiology of Bacterial Diseases
35(2)
9.3 Epidemioloy of Virus Diseases
37(5)
9.3.1 Dynamics of Host-Virus Interactions
37(1)
9.3.2 Quantitative Epidemiology
38(1)
9.3.3 Molecular Ecology and Epidemiology of Virus Diseases
39(13)
9.3.3.1 Molecular Biology of Virus Infection
40(1)
9.3.3.2 Molecular Biology of Virus Transmission
41(1)
References
42(10)
10 Crop Disease Management: Exclusion of Pathogens
52(48)
10.1 Health Status of Seeds and Propagules
52(11)
10.1.1 Certification of Seeds and Propagules
53(10)
10.1.1.1 Application of Diagnostic Techniques
53(10)
10.2 Plant Quarantines for Preventing Entry of Microbial Pathogens
63(9)
10.2.1 Principles of Plant Quarantines
63(1)
10.2.2 Functions of Regional Plant Quarantine Organizations
64(1)
10.2.3 Impact of Introduced Pathogens
65(3)
10.2.3.1 Potato Late Blight Disease
65(1)
10.2.3.2 Citrus Canker Disease
65(1)
10.2.3.3 Panama (Fusarium) Wilt Disease of Banana
66(1)
10.2.3.4 Banana Bunchy Top Disease
66(1)
10.2.3.5 Strawberry Anthracnose Disease
67(1)
10.2.3.6 Potato Virus Diseases
67(1)
10.2.4 Assessment of Efficiency of Quarantines
68(4)
10.3 Production of Disease-Free Seeds and Propagules
72(17)
10.3.1 Production of Virus-Free Plants
72(11)
10.3.1.1 Meristem-Tip Culture
72(8)
10.3.1.2 Chemotherapy
80(1)
10.3.1.3 Electrotherapy
81(1)
10.3.1.4 Cryotherapy
81(2)
10.3.2 Production of Viroid-Free Plants
83(1)
10.3.3 Production of Bacterial and Phytoplasmal Pathogen-Free Plants
84(5)
10.4 Appendix
89(2)
10.4.1 Elimination of Sweet Potato Little Leaf Phytoplasma from Sweet Potato by Cryotherapy of Shoot Tips
89(11)
10.4.1.1 Preparation of Plant Materials
89(2)
10.4.1.2 Cryotherapy of In-Vitro-Grown Shoot Tips
91(1)
References
91(9)
11 Crop Disease Management: Reduction of Pathogen Inoculum
100(42)
11.1 Reduction of Pathogen Inoculum by Cultural Practices
100(23)
11.1.1 Elimination of Infected Plants and Debris
100(2)
11.1.2 Effects of Tillage Practices
102(2)
11.1.3 Effects of Sowing Time and Planting Density
104(2)
11.1.4 Effects of Irrigation Practices
106(2)
11.1.5 Effects of Crop Nutrition
108(7)
11.1.5.1 Use of Organic Matter
110(1)
11.1.5.2 Use of Inorganic Fertilizers
111(4)
11.1.6 Effects of Other Crops
115(8)
11.1.6.1 Crop Sequence
115(6)
11.1.6.2 Monoculture
121(1)
11.1.6.3 Multiple Cropping
121(2)
11.2 Reduction of Pathogen Inoculum by Physical Techniques
123(9)
11.2.1 Treatment of Seeds and Propagules
123(9)
11.2.1.1 Heat Treatments
124(4)
11.2.1.2 Forced-Air Treatment
128(1)
11.2.1.3 Radiation and Microwave Treatments
128(1)
11.2.1.4 Use of Inorganic Mulches
129(3)
11.3 Reduction of Pathogen Inoculum by Chemical Techniques
132(1)
11.3.1 Treatment of Seeds
132(1)
11.3.1.1 Chemical Application
132(1)
11.3.1.2 Fermentation
133(1)
11.3.2 Treatment of Soils
133(1)
References
133(9)
12 Crop Disease Management: Enhancement of Genetic Resistance of Crop Plants
142(82)
12.1 Types of Disease Resistance
142(3)
12.1.1 Vertical and Horizontal Resistances
143(1)
12.1.2 Durable Resistance
144(1)
12.2 Identfication of Sources of Resistance to Crop Diseases
145(43)
12.2.1 Screening for Disease Resistance
145(43)
12.2.1.1 Assessment of Resistance by Visual Examination
145(22)
12.2.1.2 Assessment of Resistance by Quantification of Pathogen Biomass
167(3)
12.2.1.3 Molecular Basis of Resistance to Fungal Diseases
170(9)
12.2.1.4 Molecular Basis of Resistance to Bacterial Diseases
179(5)
12.2.1.5 Molecular Basis of Resistance to Virus Diseases
184(4)
12.3 Improvement of Disease Resistance Through Biotechnological Approaches
188(17)
12.3.1 Tissue and Cell Culture Techniques
189(3)
12.3.1.1 Somaclonal Variation
189(1)
12.3.1.2 In Vitro Selection for Disease Resistance
190(2)
12.3.1.3 Induction of Mutation Using Chemicals
192(1)
12.3.2 Transgenic Resistance to Crop Diseases
192(33)
12.3.2.1 Transgenic Resistance to Virus Diseases
193(4)
12.3.2.2 Transgenic Resistance to Fungal Diseases
197(6)
12.3.2.3 Transgenic Resistance to Bacterial Diseases
203(2)
References
205(19)
13 Crop Disease Management: Biological Management Strategies
224(82)
13.1 Evaluation of Biotic Agents for Biological Control Potential
225(37)
13.1.1 Fungal Biological Control Agents
225(2)
13.1.2 Bacterial Biological Control Agents
227(7)
13.1.3 Viral Biological Control Agents
234(10)
13.1.3.1 Mild Strains as Biological Control Agents
237(7)
13.1.4 Mechanisms of Action of Biological Control Activities of Biotic Agents
244(16)
13.1.4.1 Mechanisms of Action of Fungal Biological Control Agents
244(5)
13.1.4.2 Mechanisms of Action of Bacterial Biological Control Agents
249(9)
13.1.4.3 Mechanisms of Action of Mild Strains of Viruses
258(2)
13.1.5 Improvement of Biological Control Potential of Biotic Agents
260(1)
13.1.6 Transformation Crop Plants with Genes of Biological Control Agents
261(1)
13.2 Evaluation of Abiotic Agents for Biological Control Potential
262(21)
13.2.1 Effects of Addition of Organic Matter
262(3)
13.2.2 Effects of Products from Plant Sources
265(4)
13.2.3 Effects of Products from Animal Sources
269(2)
13.2.4 Effects of Organic Compounds
271(9)
13.2.5 Effects of Inorganic Compounds
280(3)
13.3 Methods of Application of Formulated Products of Biological Control Agents
283(6)
13.3.1 Treatment of Seeds
283(3)
13.3.2 Treatment of Propagules/Transplants
286(2)
13.3.3 Treatment of Soils
288(1)
13.3.4 Treatment of Foliage of Plants
289(1)
13.4 Integration of Biological Control with Other Management Practices
289(1)
13.4.1 Integration with Fertilizer Application
289(1)
13.4.2 Integration with Fungicide Application
290(1)
References
290(16)
14 Crop Disease Management: Chemical Application
306(55)
14.1 Application of Fungicides
307(34)
14.1.1 Seed/Propagule Treatment
307(9)
14.1.2 Soil Treatment
316(1)
14.1.3 Treatment of Aerial Plant Parts
317(7)
14.1.4 Development of Resistance in Fungal Pathogens to Fungicides
324(17)
14.2 Application of Chemicals Against Bacterial Diseases
341(7)
14.3 Application of Chemicals Against Virus Diseases
348(3)
14.3.1 Application of Antiviral Chemicals
348(1)
14.3.2 Chemicals Applied Against Vectors of Viruses
349(2)
References
351(10)
15 Crop Disease Management: Integration of Strategies
361(22)
15.1 Development of Integrated Disease Management Systems
361(3)
15.1.1 Selection of Strategies for Integration
362(2)
15.2 Management of Fungal Diseases
364(5)
15.2.1 Fusarium Head Blight Disease
364(1)
15.2.2 Rice Blast Disease
365(1)
15.2.3 Rice Sheath Blight Disease
366(1)
15.2.4 Late Blight Diseases of Tomato and Potato
367(2)
15.3 Management of Bacterial Diseases
369(4)
15.3.1 Rice Bacterial Leaf Blight Disease
369(2)
15.3.2 Tomato Bacterial Canker Disease
371(1)
15.3.3 Potato Scab Diseases
371(1)
15.3.4 Citrus Canker Disease
372(1)
15.4 Management of Virus Diseases
373(4)
15.4.1 Management of Seedborne Virus Diseases
374(1)
15.4.2 Management of Propagule-Borne Virus Diseases
375(2)
References
377(6)
Index
383
About the Author P. Narayanasamy, served in various capacities as Postdoctoral Research Fellow, International Rice Research Institute, Philippines, as Virus Pathologist at the Indian Agricultural Research Institute, New Delhi and as Professor and Head, at the Tamil Nadu Agricultural University, India.