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E-raamat: Mitochondrial Regulation: Methods and Protocols

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  • Formaat: EPUB+DRM
  • Sari: Methods in Molecular Biology 2310
  • Ilmumisaeg: 07-Jun-2021
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071614334
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  • Formaat: EPUB+DRM
  • Sari: Methods in Molecular Biology 2310
  • Ilmumisaeg: 07-Jun-2021
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071614334

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This fully updated edition explores the different pathways that converge into the regulation of mitochondrial function. The book integrates mitochondria with other cellular components, discussing the dynamic properties of mitochondria with an emphasis on how these processes respond to signaling events and how they affect cellular metabolism. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Mitochondrial Regulation: Methods and Protocols, Second Edition is an ideal guide for advanced undergraduates, graduates, postgraduates, and beginning researchers in the areas of molecular and cellular biology, biochemistry, and bioenergetics.
Preface v
Contributors ix
1 Isolation of Mitochondria from Liver and Extraction of Total RNA and Protein: Analyses of miRNA and Protein Expression
1(16)
Andre L. Simao
Pedro M. Borralho
Clifford J. Steer
Rui E. Castro
Cecilia M. P. Rodrigues
2 Determination of Oxidative Phosphorylation Complexes Activities
17(16)
Joao S. Teodoro
Ivo F. Machado
Carlos M. Palmeira
Anabela P. Rolo
3 BN-PAGE-Based Approach to Study Thyroid Hormones and Mitochondrial Function
33(14)
Elena Silvestri
Assunta Lombardi
Federica Cioffi
Fernando Goglia
4 Monitoring Mitochondrial Function in Mouse Embryonic Stem Cells (mESCs)
47(10)
Bibiana Correia
Maria Ines Soma
Ana F. Branco
Joao Ramalho-Santos
5 Mitochondrial Functional Assessment in Mammalian Gametes Using Fluorescent Probes
57(12)
Renata S. Tavares
Sara Escada-Rebelo
Maria M. Soares
Andreia F. Silva
Teresa Almeida-Santos
Sandra Amaral
Ana Paula Sousa
Joao Ramalho-Santos
6 Update on the Histoenzymatic Methods for Visualization of the Activity of Individual Mitochondrial Respiratory Chain Complexes in the Human Frozen Sections
69(10)
Mariusz R. Wieckowski
Maciej Pronicki
Agnieszka Karkucinska-Wieckowska
7 An Update on Isolation of Functional Mitochondria from Cells for Bioenergetics Studies
79(12)
Magdalena Lebiedzinska-Arciszewska
Lech Wojtczak
Mariusz R. Wieckowski
8 PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species
91(22)
Tess C. Leuthner
Jessica H. Hartman
Ian T. Ryde
Joel N. Meyer
9 Methods to Monitor Mitophagy and Mitochondrial Quality: Implications in Cancer, Neurodegeneration, and Cardiovascular Diseases
113(48)
Simone Patergnani
Massimo Bonora
Esmaa Bouhamida
Alberto Danese
Saverio Marchi
Giampaolo Morciano
Maurizio Previati
Gaia Pedriali
Alessandro Rimessi
Gabriele Anania
Carlotta Giorgi
Paolo Pinton
10 Analysis of Proapoptotic Protein Trafficking to and from Mitochondria
161(18)
Ignacio Vega-Naredo
Gabriela Oliveira
Teresa Cunha-Oliveira
Teresa Serafim
Vilma A. Sarddo
Paulo J. Oliveira
11 Exploring Liver Mitochondrial Function by 13C-Stable Isotope Breath Tests: Implications in Clinical Biochemistry
179(22)
Emilio Molina-Molina
Harshitha Shanmugam
Domenica Di Palo
Ignazio Grattagliano
Piero Portincasa
12 Protocols for Mitochondria as the Target of Pharmacological Therapy in the Context of Nonalcoholic Fatty Liver Disease (NAFLD)
201(46)
Ignazio Grattagliano
Agostino Di Ciaula
Jacek Baj
Emilio Molina-Molina
Harshitha Shanmugam
Gabriella Garruti
David Q.-H. Wang
Piero Portincasa
13 The Use of Reactive Oxygen Species Production by Succinate-Driven Reverse Electron Flow as an Index of Complex 1 Activity in Isolated Brown Adipose Tissue Mitochondria
247(12)
Andrea Dlaskova
Kieran J. Clarke
Mary F. Rooney
Richard K. Porter
14 Mitochondrial Regulation Assessment by 13C-NMR Isotopomer Analysis
259(12)
Francisco X. Carvalho
Barbara Guerra-Carvalho
Ivana Jarak
Rui A. Carvalho
15 Assays for Determination of Cellular and Mitochondrial NAD+ and NADH Content
271(16)
Jue Yang
Anthony A. Sauve
16 Following the Dynamism of the Mitochondrial Network in T Cells
287(14)
Arianna Di Daniele
Luca Simula
Silvia Campello
17 Measuring PGC-la and Its Acetylation Status in Mouse Primary Myotubes
301(10)
Joao A. Amorim
David A. Sinclair
Index 311