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E-raamat: Purification of Biotechnological Products: A Focus on Industrial Applications

Edited by , Edited by (King's College London, United Kingdom), Edited by (University of Sao Paulo, Brazil)
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This outstanding text focuses on providing professionals and students working in the field of pharmaceutical and biotechnology with the background necessary for development of a product or process and with the necessary rigor required by federal regulatory agencies in the pharmaceutical industry. The material will enable teachers, lecturers and professors in biotechnology to prepare courses on basic concepts and applications for purification of biotechnological products with industrial interest. These can be applied in practice, for example with projects of purification development on an industrial scale or useful unit operations for the development of bioproducts of commercial interest.

Features:

  • Purification and development of new bioproducts and improvement of those being produced.
  • Provides a background and concepts on purification of biomolecules and with an industrial perspective.
  • Allows professions to understand the entire process of developing a biopharmaceutical or bio-food, from bench to industry in biotechnology - one of the fastest-growing sectors of the economy.
  • Promotes the dissemination of information in a didactic way which is of paramount importance for interdisciplinary fields
  • Enables the reader to follow step by step stages of the development of a new biopharmaceutical, as well as allowing the optimization of existing production processes.


This unique text provides a background and concepts related to the purification of biomolecules and with an industrial perspective.

Chapter 1 Introduction

Chapter 2 Purification processes: analytical methods and enzyme stability

Chapter 3 Cell Disruption

Chapter 4 Filtration and Centrifugation

Chapter 5 Membrane Separation Processes

Chapter 6 Protein Precipitation

Chapter 7 Liquid-liquid extraction in aqueous two-phase systems

Chapter 8 Introduction to chromatography

Chapter 9 Size exclusion chromatography

Chapter 10 Iion Exchange chromatography

Chapter 11 Hydrophobic interaction chromatography

Chapter 12 Affinity Chromatography

Chapter 13 Adsorption chromatography in monolithic columns

Chapter 14 Chromatography: expanded bed adsorption (EBA)

Chapter 15 Continuous Chromatography using a Simulated Moving Bed System

Chapter 16 Chromatography scale-up

Chapter 17 Crystallization

Chapter 18 Distillation

Chapter 19 Plasmid Purification

Chapter 20 Purification of monoclonal antibodies

Chapter 21 Peptides of biotechnological interest: general concepts, production, purification and chemical characterization

Chapter 22 Integration of steps in the production of biotechnological products

Dr. Adalberto Pessoa Jr., Full professor at the School of Pharmaceutical Sciences, Biochemical-Pharmaceutical Technology Department, of the University of São Paulo and Visiting Professor at Kings College London, Institute of Pharmaceutical Science. He has experience in fermentation technology and in the purification processes of biotechnological products of interest to the pharmaceutical and food industries.

Dr. Beatriz Vahan Kilikian, nowadays retired, she was Associate Professor at the Polytechnic School of the University of São Paulo, from 1983 to 2012. She has experience in microrganisms cultivation and in the purification processes of biotechnological products of interest to the pharmaceutical, chemical and food industries.

Dr. Paul Frederick Long Full Professor of Biotechnology at King's College London and Visiting International Research Professor at the University of São Paulo. Prof Long is a microbiologist by training and his research uses a combination of bioinformatics, laboratory and field studies to discover new medicines from nature, particularly from the marine environment.
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