Essential for the laboratory, this practical manual presents a wide variety of techniques associated with the use of human CNS tissue obtained at autopsy. The book contains detailed methodologies in discrete chapters written by an expert in the specific field. It also addresses the potential for extending molecular studies in brain tissue obtained at autopsy into studies in living brain by using neuroimaging techniques. In addition, the reader is directed to suppliers of equipment and reagents that have been shown to be useful when studying human brain tissue. Topics include problems of collecting brain tissue at autopsy, how psychiatric diagnosis can be made after death and specific problems faced in using human brain tissue in modern laboratory-based techniques.Suitable for all those using human tissue in their research, this manual is of particular value to those commencing studies using human brain tissue.
Foreword vii Contributors ix The Collection of Tissue at Autopsy: Practical and Ethical Issues 1(18) Deborah A. Kittell Thomas M. Hyde Mary M. Herman Joel E. Kleinman Psychiatric Diagnosis After Death: The Problems of Accurate Diagnosis from Case History Review and Relative Interviews 19(20) Nicholas A. Keks Christine Hill Kenneth Opeskin David L Copolov Brian Dean Membrane Binding Assays: Membrane Preparation and Assay Development 39(28) Yogesh Dwivedi Ghanshyam N. Pandey The Localisation and Quantification of Molecular Changes in the Human Brain Using In Situ Radioligand Binding and Autoradiography 67(18) Brian Dean Geoffrey Pavey Siew Yeen Chai Frederick A. O. Mendelsohn In Situ Hybridisation Histochemistry: Application to Human Brain Tissue 85(22) Richard E. Loiacono Andrew L. Gundlach Immunohistochemistry Techniques Applicable for Use with Human Brain Tissue 107(20) James Vickers The Processing and Use of Postmortem Human Brain Tissue for Electron Microscopy 127(14) Rosalinda C. Roberts Lili Kung Isolating Components of Human Brain: The Purification of Aβ and the Alzheimers Amyloid Precursor Protein 141(18) Robert A. Cherny Colin L. Masters Konrad Beyreuther Ashley I. Bush Analysis of Receptor Systems in Schizophrenia Using Tissue Obtained at Autopsy and Neuroimaging 159(16) Janet Mulcrone Brain Dean Robert W. Kerwin Index 175
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