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E-raamat: Capillary Electrophoresis - Mass Spectrometry (CE-MS) - Principles and Applications: Principles and Applications [Wiley Online]

  • Formaat: 368 pages
  • Ilmumisaeg: 03-Aug-2016
  • Kirjastus: Blackwell Verlag GmbH
  • ISBN-10: 3527693807
  • ISBN-13: 9783527693801
Teised raamatud teemal:
  • Wiley Online
  • Hind: 185,03 €*
  • * hind, mis tagab piiramatu üheaegsete kasutajate arvuga ligipääsu piiramatuks ajaks
Capillary Electrophoresis - Mass Spectrometry (CE-MS) - Principles and Applications: Principles and ApplicationsSuurem pilt
  • Formaat: 368 pages
  • Ilmumisaeg: 03-Aug-2016
  • Kirjastus: Blackwell Verlag GmbH
  • ISBN-10: 3527693807
  • ISBN-13: 9783527693801
Teised raamatud teemal:
Wiley Online e-raamatudRohkem infot Wiley Online kohta
Raamatu kodulehekülg: https://onlinelibrary.wiley.com/doi/book/10.1002/9783527693801
This monograph offers the reader a complete overview on both principles and applications of CE-MS. Starting with an introductory chapter on detection in CE, also related and more specialized techniques such as electrophoretic and chromatographic preconcentration are discussed. A special emphasis is put on CE-MS interfaces, which are described in detail. In a separate chapter, attention is paid to sheath-liquid interfacing. The developments and possibilities of microchip CE-MS are also described. Applications to all relevant areas are discussed in distinct chapters, each written by experts in the respective fields. Besides applications in pharmaceutical analysis and bioanalysis, recent implementations in food science, forensic analysis, analysis of intact proteins, metabolomics and proteomics are highlighted. MS is a perfectly appropriate detection system for CE, as efficient separation is coupled to sensitive and selection detection. Moreover, MS can provide structure information on the separated compounds. CE-MS has now been developed into a strong hyphenated system complementary to LC-MS. This monograph is an unique source of knowledge for everyone dealing with and interested in CE-MS.
List of Contributors xi
1 Detection in Capillary Electrophoresis - An Introduction 1(6)
Gerhardus de Jong
1.1 UV Absorption
2(1)
1.2 Fluorescence
2(1)
1.3 Conductivity
3(1)
1.4 Mass Spectrometry
4(1)
References
4(3)
2 Electrospray Ionization Interface Development for Capillary Electrophoresis-Mass Spectrometry 7(34)
Jessica M. Risley
Caitlyn A.G. De Jong
David D.Y. Chen
2.1 A Brief Introduction to the Development of CE-MS
7(1)
2.2 Fundamentals of ESI and Electrochemical Reactions in CE-MS
8(3)
2.2.1 Principles of ESI: Converting Solvated Ions into Gaseous Ions
8(1)
2.2.2 Considerations and Conditions for CE-ESI-MS Methods
9(1)
2.2.3 Electrochemical Considerations in CE-MS
10(1)
2.3 Interface Designs
11(7)
2.3.1 Sheath-Flow Interfaces
11(4)
2.3.1.1 Flow-Through Microvial Interface
12(1)
2.3.1.2 Nanospray Sheath-Flow Interfaces
13(1)
2.3.1.3 Electrokinetically Pumped Sheath-Flow Nanospray Interface
13(2)
2.3.2 Sheathless Interfaces
15(2)
2.3.2.1 Porous-Tip Nanospray Sheathless Interface/CESI 8000
15(1)
2.3.2.2 Sheathless Porous Emitter NanoESI Interface
16(1)
2.3.3 Interface Applications/CE Mode of Separation
17(1)
2.4 Specific Interface Applications
18(2)
2.4.1 Capillary Isoelectric Focusing
18(1)
2.4.2 Glycan Analysis by CE-ESI-MS
19(1)
2.5 Conclusion
20(12)
Abbreviations
32(1)
Acknowledgments
32(1)
References
32(9)
3 Sheath Liquids in CE-MS: Role, Parameters, and Optimization 41(26)
Christian W. Klampfl
Markus Himmelsbach
3.1 Introduction
41(1)
3.2 Sheath-Liquid Functions and Sheath-Flow Interface Design
42(4)
3.2.1 Coaxial Sheath-Flow Interface
42(2)
3.2.2 Liquid Junction Interface
44(2)
3.3 Sheath-Liquid-Related Parameters and their Selection
46(7)
3.3.1 Sheath-Liquid Composition
46(5)
3.3.2 Effect of Sheath-Liquid Composition on Molecular Structures
51(1)
3.3.3 Sheath-Liquid Flow Rates and their Optimization
51(2)
3.4 Sheath Liquids for Non-ESI CE-MS Interfaces
53(4)
3.4.1 APCI and APPI
53(4)
3.5 Sheath-Flow Chemistry
57(2)
3.6 Conclusions
59(2)
References
61(6)
4 Recent Developments of Microchip Capillary Electrophoresis Coupled with Mass Spectrometry 67(36)
Gerard Rozing
4.1 Introduction
67(1)
4.2 Microchip Capillary Electrophoresis
68(4)
4.2.1 Brief Retrospective
68(1)
4.2.2 Principle of Operation of MCE
69(2)
4.2.3 Preparation and Availability of Microfluidic Chips for Capillary Electrophoresis
71(1)
4.3 Reviews on MCE and MCE-MS
72(2)
4.4 Principal Requirements for MCE-MS
74(3)
4.4.1 Electrospray Ionization
74(2)
4.4.2 Principle Layout of MCE-MS Devices
76(1)
4.5 MCEMS by Direct Off-Chip Spraying
77(1)
4.6 MCE-MS with Connected Sprayer
78(5)
4.7 MCE-MS Devices with Integrated Sprayer
83(7)
4.8 Multidimensional MCE-MS Devices
90(1)
4.9 Conclusions and Perspectives
91(5)
References
96(7)
5 On-Line Electrophoretic, Electrochromatographic, and Chromatographic Sample Concentration in CE-MS 103(26)
Joselito P. Quirino
5.1 Introduction
103(1)
5.2 Electrophoretic and Electrochromatographic Sample Concentration or Stacking
104(11)
5.2.1 Electrophoretic Stacking Techniques
104(9)
5.2.1.1 Transient Isotachophoresis or t-ITP
105(2)
5.2.1.2 Field-Amplified/Enhanced Stacking
107(3)
5.2.1.3 Dynamic pH Junction
110(3)
5.2.2 Electrochromatographic Sample Concentration
113(2)
5.2.2.1 Sweeping
113(1)
5.2.2.2 Analyte Focusing by Micelle Collapse or AFMC
114(1)
5.2.2.3 Micelle to Solvent Stacking or MSS
115(1)
5.3 On-line/In-line SPE with CE-MS
115(6)
5.3.1 On-line SPE
116(1)
5.3.2 In-line SPE
117(4)
5.4 Conclusion
121(1)
Acknowledgment
122(1)
References
122(7)
6 CE-MS in Drug Analysis and Bioanalysis 129(30)
Julie Schappler
Victor Gonzalez-Ruiz
Serge Rudaz
6.1 Introduction
129(3)
6.2 CE-MS in Drug Analysis
132(9)
6.2.1 Impurity Profiling
134(1)
6.2.2 Chiral Analysis
135(1)
6.2.3 Determination of Drugs' Physicochemical Properties
136(5)
6.2.3.1 pKa and log P
137(3)
6.2.3.2 Plasma Protein Binding
140(1)
6.3 CE-MS in Bioanalysis
141(4)
6.3.1 Selectivity Issues and Matrix Effects
142(2)
6.3.2 Sample Preparation
144(1)
6.4 CE-MS in Drug Metabolism Studies
145(3)
6.4.1 Electrophoretically Mediated Microanalysis
146(1)
6.4.2 Targeted in vitro Metabolism Assays
147(1)
6.5 Quantitative Aspects in CE-MS
148(3)
6.5.1 Instrumental Aspects
148(1)
6.5.2 Methodological Aspects
149(2)
6.6 Conclusions
151(1)
Abbreviations
151(1)
References
152(7)
7 CE-MS for the analysis of intact proteins 159(34)
Rob Haselberg
Govert W. Somsen
7.1 Introduction
159(2)
7.2 CE of Intact Proteins
161(3)
7.2.1 CE Modes
161(1)
7.2.2 Preventing Protein Adsorption
161(3)
7.3 MS Detection of Intact Proteins
164(4)
7.3.1 Ionization Modes
164(3)
7.3.2 Mass Analyzers
167(1)
7.4 Applications of Intact Protein CE-MS
168(18)
7.4.1 Biopharmaceuticals
168(6)
7.4.2 Glycoproteins
174(3)
7.4.3 Protein-Ligand Interactions
177(3)
7.4.4 Metalloproteins
180(2)
7.4.5 Top-Down Protein Analysis
182(2)
7.4.6 Other Selected Applications
184(2)
7.5 Conclusions
186(1)
Abbreviations
187(1)
References
188(5)
8 CE-MS in Food Analysis and Foodomics 193(24)
Tanize Acunha
Clara Ibanez
Virginia Garcia-Canas
Alejandro Cifuentes
Carolina Simo
8.1 Introduction: CE-MS, Food Analysis, and Foodomics
193(16)
8.1.1 CE-MS and Food Safety
194(7)
8.1.2 CE-MS in Food Quality and Authenticity
201(3)
8.1.3 CE-MS and Foodomics
204(5)
8.2 Concluding Remarks
209(1)
Acknowledgments
209(1)
References
210(7)
9 CE-MS in Forensic Sciences with Focus on Forensic Toxicology 217(76)
Nadia Porpiglia
Elena Giacomazzi
Rossella Gottardo
Franco Tagliaro
9.1 Introduction
217(1)
9.2 Sample Preparation of Forensically Relevant Matrices
218(7)
9.2.1 Blood
219(2)
9.2.2 Urine
221(2)
9.2.3 Hair
223(1)
9.2.4 Saliva
224(1)
9.3 Separation Modes and Analytical Conditions
225(9)
9.3.1 Capillary Zone Electrophoresis
225(1)
9.3.2 Capillary Isotachophoresis
226(1)
9.3.3 Micellar Electrokinetic Chromatography
227(1)
9.3.4 Capillary Electrochromatography
228(1)
9.3.5 Capillary Gel Electrophoresis
228(1)
9.3.6 Chiral Separation
228(3)
9.3.7 Analytical Conditions
231(3)
9.4 Applications
234(44)
9.4.1 Forensic Toxicology
234(23)
9.4.1.1 Drugs of Abuse
235(12)
9.4.1.2 Alcohol Abuse Biomarkers
247(4)
9.4.1.3 Doping
251(6)
9.4.2 Trace Evidence Analysis
257(12)
9.4.2.1 Gunshot Residues, Explosives, and Chemical Weapons
259(5)
9.4.2.2 Inks
264(1)
9.4.2.3 Dyes
265(3)
9.4.2.4 Textile Fibers
268(1)
9.4.3 Forensic DNA
269(3)
9.4.4 Occupational and Environmental Health
272(25)
9.4.4.1 Toxins
274(1)
9.4.4.2 Venoms
275(1)
9.4.4.3 Pesticides
276(2)
9.5 Conclusions
278(2)
References
280(13)
10 CE-MS in Metabolomics 293(22)
Akiyoshi Hirayama
Tomoyoshi Soga
10.1 Introduction
293(1)
10.2 Sample Preparation and MS Systems
294(3)
10.3 Application
297(11)
10.3.1 Blood
298(4)
10.3.2 Urine
302(1)
10.3.3 Other Biofluids
303(1)
10.3.4 Cell Cultures
304(1)
10.3.5 Tissue
305(3)
10.3.6 Plants
308(1)
10.4 Conclusions
308(2)
Acknowledgments
310(1)
References
310(5)
11 CE-MS for Clinical Proteomics and Metabolomics: Strategies and Applications 315(30)
Rawi Ramautar
Philip Britz-McKibbin
11.1 Introduction
315(2)
11.2 Clinical Proteomics
317(11)
11.2.1 Sample Pretreatment
317(2)
11.2.2 Separation Conditions
319(3)
11.2.3 Data Analysis and Validation
322(3)
11.2.4 Comparison of CE-MS with Other Techniques
325(3)
11.3 Clinical Metabolomics
328(11)
11.3.1 CE-MS Strategies for Clinical Metabolomics
328(7)
11.3.2 Data Analysis and Clinical Validation
335(2)
11.3.3 Comparison of CE-MS with Other Techniques
337(2)
11.4 Conclusions and Perspectives
339(1)
Abbreviations
339(1)
Acknowledgments
340(1)
References
340(5)
Index 345
Gerhardus (Ad) de Jong studied chemistry in Utrecht, the Netherlands, and received his Ph.D. at the Free University of Amsterdam. He worked for Duphar, a pharmaceutical company in Weesp, the Netherlands, in the department Analytical Research. Subsequently, he returned to the Free University as associate professor in the Department of Analytical Chemistry. In 1990, he again moved to the pharmaceutical industry and was appointed to section head Chromatography of Solvay Duphar. In 1995, he was appointed professor in Analytical Chemistry and Pharmaceutical Analysis at the University Centre for Pharmacy, Groningen, the Netherlands, and since 2001, he is professor in Pharmaceutical Analysis at the Department of Pharmaceutical Sciences, Utrecht University. Prof. de Jong is (co-)author of more than 300 papers in various scientific journals and some book chapters. He is co-author of a book on chromatography and was editor of the Journal of Chromatography B from 1998 to 2003. His research interests encompass separation techniques and their applications in pharmaceutical analysis, bioanalysis and analysis of biomacromolecules.
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