Muutke küpsiste eelistusi

E-raamat: Cellular Analysis by Atomic Force Microscopy [Taylor & Francis e-raamat]

  • Formaat: 240 pages, 12 Tables, black and white; 19 Illustrations, color; 97 Illustrations, black and white
  • Ilmumisaeg: 27-Jun-2017
  • Kirjastus: Pan Stanford Publishing Pte Ltd
  • ISBN-13: 9781315364803
Teised raamatud teemal:
  • Taylor & Francis e-raamat
  • Hind: 143,10 €*
  • * hind, mis tagab piiramatu üheaegsete kasutajate arvuga ligipääsu piiramatuks ajaks
  • Tavahind: 204,43 €
  • Säästad 30%
Cellular Analysis by Atomic Force MicroscopySuurem pilt
  • Formaat: 240 pages, 12 Tables, black and white; 19 Illustrations, color; 97 Illustrations, black and white
  • Ilmumisaeg: 27-Jun-2017
  • Kirjastus: Pan Stanford Publishing Pte Ltd
  • ISBN-13: 9781315364803
Teised raamatud teemal:
Taylor & Francis e-raamatudRohkem infot Taylor & Francis e-raamatute kohta
Raamatu kodulehekülg: https://www.taylorfrancis.com/books/9781315364803

Despite substantial evidence showing the feasibility of Atomic Force Microscopy (AFM) to identify cells with altered elastic and adhesive properties, the use of this technique as a complementary diagnostic method remains controversial. This book is designed to be a practical textbook that teaches how to assess the mechanical characteristics of living, individual cells by AFM. Following a step-by-step approach, it introduces the methodology of measurements in the case of both determination of elastic properties and quantification of adhesive properties.

Preface xi
1 Introduction
1(22)
1.1 Cell Ability to Deform
2(9)
1.1.1 Monitoring Chitosan Effect on Cancerous Cells
6(2)
1.1.2 Mechanosensitivity of Cancerous Cells
8(2)
1.1.3 Stiffness as Cancer Grades
10(1)
1.2 Cell Ability to Adhere
11(12)
1.2.1 Specific Interactions in Living Cells
12(11)
2 Cell Structure and Functions
23(30)
2.1 Extracellular Matrix
25(4)
2.1.1 The ECM Proteins
26(2)
2.1.2 Proteoglycans
28(1)
2.1.3 Other Components of the ECM---Hyaluronan
29(1)
2.2 Cell Membrane
29(8)
2.2.1 Membrane Structure
30(1)
2.2.1.1 Lipids
31(4)
2.2.1.2 Proteins
35(2)
2.3 Surface Receptors
37(9)
2.3.1 Integrins
38(1)
2.3.2 Cadherins
39(3)
2.3.3 Selectins
42(1)
2.3.4 Immunoglobulin Family
43(1)
2.3.5 Glycans
44(2)
2.4 Cytoskeleton
46(7)
2.4.1 Actin Filaments
47(1)
2.4.2 Microtubules
48(1)
2.4.3 Intermediate Filaments
49(4)
3 Principles of Atomic Force Microscopy
53(42)
3.1 Principles of the AFM Operation
53(14)
3.1.1 Cantilevers
55(3)
3.1.2 Detection System of Cantilever Deflection
58(2)
3.1.3 Feedback Loop
60(1)
3.1.4 Scanning and Positioning System
60(7)
3.2 Force Spectroscopy
67(28)
3.2.1 Calibration
69(1)
3.2.1.1 Photodetector sensitivity
69(2)
3.2.1.2 Correction factor κ for PSD sensitivity
71(1)
3.2.1.3 Spring constant
72(9)
3.2.1.4 Force versus sample-distance conversion
81(2)
3.2.1.5 Hydrodynamic drag
83(2)
3.2.1.6 Force detection limit
85(2)
3.2.1.7 Scanner linearization
87(2)
3.2.1.8 Scanner velocity determination
89(6)
4 Quantification of Cellular Elasticity
95(54)
4.1 Materials Properties and Theoretical Models
95(12)
4.1.1 Basic Terms Used in Material Mechanics
95(3)
4.1.2 Rheological Models
98(1)
4.1.2.1 Mechanical behavior of soft materials
99(3)
4.1.2.2 Soft glassy model
102(2)
4.1.2.3 Tensegrity theory
104(2)
4.1.2.4 Classification of material properties based on indentation
106(1)
4.2 Single-Cell Deformability Measurements
107(42)
4.2.1 Experimental Conditions for the AFM
110(3)
4.2.2 Criteria for Force Curve Selection
113(3)
4.2.3 Force versus Indentation Curves
116(1)
4.2.4 Determination of Young's Modulus
117(2)
4.2.4.1 The final Young's modulus calculations
119(2)
4.2.5 Depth-Sensing Analysis
121(5)
4.2.6 Stiffness Tomography
126(1)
4.2.7 Distinct Factors Influencing Cell's Elasticity
127(1)
4.2.7.1 Calibration-based discrepancy
128(1)
4.2.7.2 Variability stemming from cell-related factors
129(3)
4.2.7.3 The influence of the AFM experimental conditions
132(2)
4.2.7.4 Discrepancies stemming from the Hertz contact mechanics theory
134(2)
4.2.7.5 The contact point determination and data analysis
136(2)
4.2.7.6 Substrate properties
138(1)
4.2.7.7 Comparing properties of human bladder cancer cells
139(10)
5 Adhesive Properties Studied by AFM
149(68)
5.1 Unbinding of Molecules: Theoretical Basis
150(17)
5.1.1 Brief Introduction to Kramer's Theory
150(2)
5.1.2 Force-Induced Single Bond Disruption
152(4)
5.1.3 Hierarchic Crossing through the Energy Barriers
156(2)
5.1.4 The Energy Barrier Height
158(1)
5.1.5 Multiple Bond Rupture
159(1)
5.1.5.1 Sequential bond rupture: the "zipper-like" model
159(2)
5.1.5.2 Sequential bond rupture: the "parallel-like" model
161(2)
5.1.6 Comparing Unbinding Properties of Two Single Complexes
163(3)
5.1.7 Other Theoretical Models for Single Molecule Interactions
166(1)
5.1.7.1 Dudko--Hummer--Szabo model
166(1)
5.1.7.2 Friddle--Noy--De Yoreo model
167(1)
5.2 AFM Measurements of Adhesive Properties
167(23)
5.2.1 Attachment of Molecules to Desired Surfaces
169(1)
5.2.1.1 AFM probe functionalization
169(5)
5.2.1.2 Preparation of a cell probe
174(2)
5.2.1.3 Cells preparation for the AFM measurements
176(1)
5.2.2 Inhibition of Binding Site
176(2)
5.2.3 The Unbinding of Molecular Complexes: Force Curves
178(2)
5.2.4 Parameters Derived from a Single Force Curve
180(1)
5.2.4.1 The pull-off force and force histogram
181(3)
5.2.4.2 Relation between the unbinding force and the number of ruptured bonds
184(2)
5.2.4.3 The rupture length and its histogram
186(1)
5.2.4.4 The number of ruptured bonds
187(2)
5.2.4.5 The unbinding probability
189(1)
5.3 Single Molecule Interaction in Living Cells: A Case Study
190(13)
5.3.1 Properties of N-Cadherin in Bladder Cancer Studied by AFM
190(1)
5.3.1.1 Shape of the force curves for Ncad--GC4 complex
191(1)
5.3.1.2 Unbinding force dependence on loading rate
192(1)
5.3.1.3 Force histograms for Ncad--GC4 complex
193(2)
5.3.1.4 Multiple unbinding in human bladder cells
195(2)
5.3.1.5 Bell--Evans model parameters
197(1)
5.3.1.6 Energy landscape reconstruction
198(1)
5.3.1.7 Kinetics profiles
199(1)
5.3.1.8 Specificity of the Ncadh--GC4 complex
200(2)
5.3.1.9 Summary for Ncadh--CG4 complex
202(1)
5.4 Living Cell as a Probe
203(14)
6 Conclusions
217(4)
Index 221
Lekka, Malgorzata
Püsilink: https://www.kriso.ee/db/9781315364803_pe.html
Märksõnad: