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E-raamat: Molecular Biological and Immunological Techniques and Applications for Food Chemists [Wiley Online]

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  • Formaat: 496 pages
  • Ilmumisaeg: 12-Jan-2010
  • Kirjastus: John Wiley & Sons Inc
  • ISBN-10: 470637684
  • ISBN-13: 9780470637685
  • Wiley Online
  • Hind: 169,17 €*
  • * hind, mis tagab piiramatu üheaegsete kasutajate arvuga ligipääsu piiramatuks ajaks
  • Formaat: 496 pages
  • Ilmumisaeg: 12-Jan-2010
  • Kirjastus: John Wiley & Sons Inc
  • ISBN-10: 470637684
  • ISBN-13: 9780470637685
A guide to using molecular biology and immunological methods for the analysis of food Many of the analytical problems that food chemists face in the lab cannot be solved by chemistry alone, and so analytical chemists are turning to molecular biology and immunology for alternative approaches. Molecular Biological and Immunological Techniques and Applications for Food Chemists comprehensively explains the most important molecular biology and immunology methods, and illustrates their application in food analysis.

Written by a distinguished group of experts, the coverage includes:





Molecular Biological Methodstechniques explained, laboratory layout, PCR, real-time PCR, RFLP, SSCP, and sequencing Molecular Biology Applicationsmeat, genetically modified organisms (GMOs), food allergens, offal, and fish



Immunological Methodstechniques explained and antibody-based detection methods



Immunology Applicationsanimal speciation, international food allergen regulations (except Japanese), Japanese regulations and buckwheat allergen detection, egg allergen detection, soy allergen detection, milk allergen detection, gluten allergen detection, nut allergen detection, fish allergen detection, lupin allergen detection, mustard allergen detection, and celery allergen detection





Clearly written and consistently edited to provide information to a wide range of readers, Molecular Biological and Immunological Techniques and Applications for Food Chemists offers an up-to-date reference for food scientists in government and industry, policymakers, and graduate-level students of food science, technology, and engineering.
CONTRIBUTORS xi
PREFACE xiii
PART Ia MOLECULAR BIOLOGICAL METHODS: TECHNIQUES EXPLAINED
1. Molecular Biology Laboratory Layout
3
Rainer Schubbert
2. Polymerase Chain Reaction
41
Hermann Broil
3. Quantitative Real-Time PCR
59
Hermann Broil
4. Polymerase Chain Reaction–Restriction Fragment Length Polymorphism Analysis
85
Klaus Pietsch and Hans-Ulrich Waiblinger
5. Single-Stranded Conformation Polymorphism Analysis
105
Hartmut Rehbein
6. Sequencing
119
Rainer Schubbert
PART Ib MOLECULAR BIOLOGICAL METHODS: APPLICATIONS
7. Meat
135
Ines Laube
8. Genetically Modified Organisms
157
Bert Popping
9. Detection of Food Allergens
175
Carmen Diaz-Amigo and Bert Popping
10. Offal
199
Neil Harris
11. Aquatic Food
209
Hartmut Rehbein
PART IIa IMMUNOLOGICAL METHODS: TECHNIQUES EXPLAINED
12. Antibody-Based Detection Methods: From Theory to Practice
223
Carmen Diaz-Amigo
PART IIb IMMUNOLOGICAL METHODS: APPLICATIONS
13. Animal Specification in Speciation
249
Bruce W. Ritter and Laura Allred
14. International Regulatory Environment for Food Allergen Labeling
267
Samuel Benrejeb Godefroy and Bert Popping
15. Japanese Regulations and Buckwheat Allergen Detection
293
Hiroshi Akiyama, Shinobu Sakai, Reiko Adachi, and Reiko Teshima
16. Egg Allergen Detection
311
Masahiro Shoji
17. Soy Allergen Detection
335
Marcello Gatti and Cristina Ferretti
18. Milk Allergen Detection
349
Sabine Baumgartner
19. Gluten Detection
359
Ulrike Immer and Sigrid Haas-Lauterbach
20. Nut Allergen Detection
377
Richard Fielder, Warren Higgs, and Katie Barden
21. Fish Allergen Detection
407
Christiane Kruse &este
22. Lupin Allergen Detection
423
Christiane Kruse Fceste
23. Mustard Allergen Detection
445
Anne E. Ryan and Michael S. Ryan
24. Celery Allergen Detection
451
Charlotta Engdahl Axelsson
INDEX 459
BERT POPPING is the Director of Molecular Biology and Immunology at Eurofins Scientific Group. Dr. Popping is a member of the AOAC International Presidential Taskforce on food allergens, the AOAC General Referee for Food Allergen Methods, leading the Allergen Working Group within the European Sixth Framework Programme MoniQA, and is a member of several national and international working groups. He is a member of the Scientific Advisory Panel for the American Association of Cereal Chemists (AACC), and is on the Board of Directors for the AOAC Research Institute. CARMEN DIAZ-AMIGO is a Research Biologist with the Center for Food Safety and Applied Nutrition at the U.S. Food and Drug Administration. She has been involved in the strategic research planning of the FDA. She is a member of several national and international working groups including the AOAC International Presidential Taskforce onfood allergens, and the Allergen Working Group within the European Sixth Framework Programme MoniQA.

KATRIN HOENICKE is a Food Chemist and member of the management of the Eurofins Competence Center for Contaminants in Foods, WEJ Contaminants, in Hamburg, Germany. She is a member of several national and international working groups including the AOAC European Executive Committee.