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Circulating Tumor Cells: Methods and Protocols Second Edition 2026 [Hardback]

  • Format: Hardback, 456 pages, height x width: 254x178 mm, 73 Illustrations, color; 1 Illustrations, black and white
  • Series: Methods in Molecular Biology
  • Pub. Date: 09-Jun-2026
  • Publisher: Humana
  • ISBN-10: 1071650491
  • ISBN-13: 9781071650493
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  • Format: Hardback, 456 pages, height x width: 254x178 mm, 73 Illustrations, color; 1 Illustrations, black and white
  • Series: Methods in Molecular Biology
  • Pub. Date: 09-Jun-2026
  • Publisher: Humana
  • ISBN-10: 1071650491
  • ISBN-13: 9781071650493
Other books in subject:

This second editions explores new and updated approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs). Chapters detail comprehensive protocols for the capture of CTCs via filtration, density gradient centrifugation, microfluidic, immunomagnetic separation of CTCs, detection of CTCs by immunocytochemistry, fluorescence in situ hybridization, flow cytometry, assays designed for genomic characterization, and functional analyses of CTCs. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Circulating Tumor Cells: Methods and Protocols, Second Edition aims to be comprehensive guide for researchers in the field.

Detection and Separation of Circulating Tumor Cells Using Magnetic
Fluorescent Nanoparticles.- Optimized Workflow for Sensitive Detection of
Circulating Tumor Cells Using qPCR and Pre-Amplification to Minimize
Cross-Contamination.- Highly efficient capture of sarcoma cells in
microfluidics devices.- Detection of Circulating tumor cells with an
Epithelial-to-Mesenchymal Transition Phenotype.- Phenotypic characterization
and sorting of circulating tumor cells (CTCs) using a digital image-based
sorter.- A New Filter for Isolation of Circulating Tumor Cells by Only Blood
Filtration.- Androgen Receptor Variant 7 (AR-V7) transcript expression in
circulating tumor cells.- Androgen Receptor Variant 7 (AR-V7) protein
detection in circulating tumor cells by immunocytostaining.- Androgen
receptor splice variant-7 expression analysis in circulating tumor cells:
methodology and application of AdnaTest in prostate cancer research.-
Nanomechanical Phenotyping of Circulating Tumor Cells with atomic force
microscopy.- Model Circulating Tumor Cells Induced by Mechanical
Stress.- Characterization of circulating tumor cells and other rare cells in
blood using the RareCyte platform.- Liquid biopsies and molecular imaging for
precise cancer management.- Small cell lung cancer: screening of kinase
inhibitors.- Cell-interaction analysis and functional studies using
circulating tumor cell models.-Negative Enrichment and Isolation of
Circulating Tumor Cells from Cryopreserved Blood Fractions for Whole Genome
Amplification.- Isolation and sequencing of single circulating tumor
cells.- Micro RNA Sequencing in circulating tumor cells.- Single-cell RNA
sequencing of pancreatic circulating tumor cells.- Utilizing magnetic
microraft technology for single-cell isolation of CTCs from whole blood for
scRNA-seq transcriptomic characterization .- Advancements in circulating
tumor cell expansion ex vivo: a matured technology?.- Culture and expansion
of circulating tumor cells from breast malignant effusions as organoids.-
Bioinformatics of shallow whole genome sequencing data of circulating tumor
cells to inform cancer diagnosis and treatment.-mAutomated analysis of liquid
biopsy using deep learning: detecting circulating tumor cells and
cancer-associated fibroblasts.- Artificial Intelligence for CELLSEARCH image
analysis.- RNA-Seq and XAI can be used as tools to aid pathologists in the
process of cancer diagnosis.- New strategies to uncover genes involved in the
development of prostate cancer in families with multiple cases.