Preface |
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ix | |
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1 | (6) |
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7 | (18) |
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7 | (8) |
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15 | (1) |
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Information flow: gene expression |
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16 | (5) |
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Gene structure and organization |
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21 | (4) |
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25 | (16) |
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25 | (1) |
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Overview of the procedures |
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26 | (3) |
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29 | (1) |
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30 | (2) |
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Polymerase chain reaction |
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32 | (1) |
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Extraction and purification of nucleic acids |
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33 | (3) |
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Detection and quantitation of nucleic acids |
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36 | (1) |
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37 | (4) |
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41 | (20) |
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Restriction endonucleases |
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41 | (6) |
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47 | (6) |
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Modification of restriction fragment ends |
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53 | (4) |
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Other ways of joining DNA molecules |
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57 | (4) |
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61 | (34) |
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61 | (8) |
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Vectors based on the lambda bacteriophage |
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69 | (9) |
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78 | (1) |
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79 | (2) |
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81 | (3) |
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Vectors for cloning and expression in eukaryotic cells |
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84 | (8) |
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Supervectors: YACs and BACs |
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92 | (2) |
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94 | (1) |
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Genomic and cDNA libraries |
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95 | (22) |
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96 | (9) |
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Growing and storing libraries |
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105 | (1) |
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106 | (7) |
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Random, arrayed and ordered libraries |
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113 | (4) |
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117 | (18) |
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Screening libraries with gene probes |
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117 | (11) |
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Screening expression libraries with antibodies |
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128 | (2) |
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130 | (1) |
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Characterization of plasmid clones |
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131 | (4) |
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Polymerase chain reaction |
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135 | (24) |
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136 | (4) |
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140 | (4) |
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144 | (2) |
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146 | (1) |
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Reverse-transcription PCR |
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146 | (1) |
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Rapid amplification of cDNA ends |
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147 | (2) |
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149 | (5) |
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154 | (5) |
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Characterization of a cloned gene |
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159 | (42) |
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159 | (8) |
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Databank entries and annotation |
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167 | (6) |
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173 | (5) |
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178 | (12) |
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190 | (6) |
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196 | (5) |
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Analysis of gene expression |
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201 | (20) |
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201 | (8) |
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Methods for studying the promoter |
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209 | (4) |
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Regulatory elements and DNA-binding proteins |
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213 | (4) |
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217 | (4) |
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Products from native and manipulated cloned genes |
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221 | (30) |
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Factors affecting expression of cloned genes |
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222 | (5) |
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Expression of cloned genes in bacteria |
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227 | (8) |
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Expression in eukaryotic host cells |
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235 | (4) |
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239 | (3) |
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242 | (5) |
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247 | (4) |
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251 | (36) |
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251 | (10) |
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261 | (8) |
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269 | (2) |
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271 | (2) |
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Relating genes and functions: genetic and physical maps |
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273 | (3) |
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Transposon mutagenesis and other screening techniques |
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276 | (9) |
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285 | (2) |
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Analysis of genetic variation |
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287 | (26) |
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Single nucleotide polymorphisms |
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288 | (3) |
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291 | (2) |
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Other methods for studying variation |
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293 | (7) |
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300 | (5) |
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305 | (8) |
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313 | (20) |
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Analysing transcription; transcriptomes |
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313 | (6) |
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319 | (7) |
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Translational analysis; proteomics |
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326 | (3) |
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Post-translational analysis: protein interactions |
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329 | (3) |
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Integrative studies; systems biology |
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332 | (1) |
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Modifying organisms; transgenics |
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333 | (22) |
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Modification of bacteria and viruses: live vaccines |
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333 | (4) |
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337 | (1) |
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338 | (9) |
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Applications of transgenic animals |
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347 | (4) |
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Transgenic plants and their applications |
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351 | (4) |
Glossary |
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355 | (18) |
Bibliography |
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373 | (2) |
Index |
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375 | |