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E-raamat: Emerging Tools for Single-Cell Analysis: Advances in Optical Measurement Technologies [Wiley Online]

Edited by (University of Illinois Biotechnology Center, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA), Edited by (Purdue University Cytometry Laboratories, Purdue University, West Lafayette, Indiana, USA)
  • Formaat: 359 pages
  • Sari: Cytometric Cellular Analysis
  • Ilmumisaeg: 22-May-2000
  • Kirjastus: John Wiley & Sons Inc
  • ISBN-10: 471224847
  • ISBN-13: 9780471224846
  • Wiley Online
  • Hind: 228,91 €*
  • * hind, mis tagab piiramatu üheaegsete kasutajate arvuga ligipääsu piiramatuks ajaks
  • Formaat: 359 pages
  • Sari: Cytometric Cellular Analysis
  • Ilmumisaeg: 22-May-2000
  • Kirjastus: John Wiley & Sons Inc
  • ISBN-10: 471224847
  • ISBN-13: 9780471224846
American (and one German) scientists in engineering and biology explore the direction of current cytometric technology, stressing the applications of these advances in cell measurement and cell- sorting. Topics include microfabricated fluidic devices, fluorescence imaging, confocal imaging, white-light scanning digital microscopy, and new camera technologies for cytometry applications. Annotation c. Book News, Inc., Portland, OR (booknews.com)

The resurgence of interest in high-resolution evaluation of single-cell properties has led to examining where current technology stands at the beginning of a new millennium. Engineers and scientists have produced significant advances in cytometric technologies in just the past few years. Emerging Tools for Single-Cell Analysis: Advances in Optical Measurement Technologies stresses the applications and theories behind some of these advances in cell measurement and cell- sorting technologies.

Rapid assessment of the proper function of cells and molecular processes within cells is essential. To that end, new and varying technologies present important diagnostic and prognostic tools relevant to a variety of diseases. Future developments in miniaturization of electronics, micro- and nanomachines, and biomedical engineering are certain to impact cell biology. New analytical technologies are revolutionizing our ability to functionally characterize, isolate, and manipulate single cells. This timely book offers researchers and design engineers much-needed information as they further develop technologies for cell analysis.

By comparing and contrasting various approaches, the authors explain how those technologies converge toward similar goals: evaluating the properties of cells and sorting cells on those properties using optically-based measurement systems. Emerging Tools for Single-Cell Analysis offers scientists and engineers a vision of the exciting possibilities that exist as new technologies are applied to single-cell analysis,

Preface vii Contributors ix Cell-Sorting Technology 1(20) Gary Durack High-Speed Cell Sorting 21(28) Ger van den Engh Rare-Event Detection and Sorting of Rare Cells 49(24) James F. Leary Applications of High-Speed Sorting for CD34+ Hematopoietic Stem Cells 73(22) Thomas Leemhuis David Adams Microfabricated Fluidic Devices for Single-Cell Handling and Analysis 95(20) David J. Beebe Single DNA Fragment Detection by Flow Cytometry 115(24) Robert C. Habbersett James H. Jett Richard A. Keller Fluorescence Lifetime Imaging: New Microscopy Technologies 139(36) Weiming Yu William W. Mantulin Enrico Gratton Fluorescence Lifetime Flow Cytometry 175(22) John A. Steinkamp Application of Fluorescence Lifetime and Two-Photon Fluorescence Cytometry 197(24) Donald J. Weaver Jr. Gary Durack Edward W. Voss Jr. Anu Cherukuri Probing Deep-Tissue Structures by Two-Photon Fluorescence Microscopy 221(18) Chen-Yuan Dong Ki Hean Kim Christof Buehler Lily Hsu Hyun Kim Peter T. C. So Barry R. Masters Enrico Gratton Irene E. Kochevar Limits of Confocal Imaging 239(32) James B. Pawley Scanning Near-Field Optical Imaging and Spectroscopy in Cell Biology 271(20) Vinod Subramaniam Achim K. Kirsch Attila Jenei Thomas M. Jovin White-Light Scanning Digital Microscopy 291(16) J. Paul Robinson Ben Gravely Illumination Sources 307(16) Howard M. Shapiro Camera Technologies for Cytometry Applications 323(16) Kenneth Castleman Index 339
Gary Durack is a biomedical engineer, inventor, and successful technology entrepreneur. Gary began his career at the Coulter Corporation, a world leader in IVD instrumentation.

J. Paul Robinson is the editor of Emerging Tools for Single-Cell Analysis: Advances in Optical Measurement Technologies, published by Wiley.