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E-raamat: Flow Cytometry Applications in Cell Culture

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  • Formaat: 344 pages
  • Ilmumisaeg: 24-Jul-2020
  • Kirjastus: CRC Press Inc
  • Keel: eng
  • ISBN-13: 9781000148503
  • Formaat - EPUB+DRM
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  • Formaat: 344 pages
  • Ilmumisaeg: 24-Jul-2020
  • Kirjastus: CRC Press Inc
  • Keel: eng
  • ISBN-13: 9781000148503

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This invaluable resource presents practical biotechnological applications of flow cytometry techniques for the study of animal, plant, and microbial cells - explaining methodologies for sample preparation, staining, and analysis.
Generously illustrated and containing key bibliographic citations, Flow Cytometry Applications in Cell Culture is an excellent reference for biochemists, molecular and cell biologists, flow cytometrists, microbiologists, bioscientists, biochemical engineers, cytologists, immunologists, fermentation engineers, and graduate-level and continuing-education students in these disciplines.

Presents the latest research in flow cytometry for investigation and monitoring of cellular processes for biotechnological purposes, emphasizing the practical aspects of efficient operation of flow cytometric techniques. Topics include aspects of cell variability encountered in cell culture processes; quantitative analysis of heterogeneous populations; cell cycle analysis of animal cell cultures; and the use of flow cytometry to monitor and improve cell line selection. Discusses applications including analysis of baculovirus-infected cell culture, detecting cellular and subcellular changes in apoptosis, and antibody secretion assays using gel microdrops and flow cytometry. Of interest to microbiologists, biochemists, and biochemical engineers. Annotation copyright Book News, Inc. Portland, Or.
Monitoring the proliferative capacity of cultured animal cells by cell cycle analysis; use of flow cytometry for monitoring antibody productivity and isolating high-secreting hybridoma cells; antibody secretion assays using gel microdrops and flow cytometry; high resolution cell cycle analysis of cell cycle-regulated gene expression; stability of monoclonal antibody production in hybridoma cell culture; flow cytometric studies of osmotically stressed and sodium butyrate-treated hybridoma cells; flow cytometric analysis of cells obtained from human bone marrow cultures; measurement of intracellular pH during the cultivation of hybridoma cells in batch and continuous cultures; the relationship between intracellular pH and cell cycle in cultured animal cells using SNARF-1 indicator; assessment of cell viability in mammalian cell lines; analysis of apoptosis by flow cytometry; monitoring baculovirus infection and protein expression in insect cells; use of protein distribution to analyze budding yeast population structure and cell cycle progression; cell cycle analysis of microorganisms; bacterial characterization by flow cytometry; assessment of viability of bacteria by flow cytometry; advances in the flow cytometric characterization of plant cells and tissues.
Mohamed Al-Rubeai, A. Nicholas Emery