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E-raamat: Flow Cytometry Applications in Cell Culture [Taylor & Francis e-raamat]

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  • Formaat: 344 pages
  • Ilmumisaeg: 05-Sep-2019
  • Kirjastus: CRC Press
  • ISBN-13: 9781003067467
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Flow Cytometry Applications in Cell CultureSuurem pilt
  • Formaat: 344 pages
  • Ilmumisaeg: 05-Sep-2019
  • Kirjastus: CRC Press
  • ISBN-13: 9781003067467
Teised raamatud teemal:
Taylor & Francis e-raamatudRohkem infot Taylor & Francis e-raamatute kohta
Raamatu kodulehekülg: https://www.taylorfrancis.com/books/9781003067467
This work present practical, biotechnological applications of flow cytometry techniques for the study of animal, plant and microbial cells, explaining methodologies for sample preparation, staining and analysis. It discusses cell variability in cell culture processes and shows how the quantitative analysis of heterogeneous populations aids in the biotechnological exploitation of cells.
Preface iii
Contributors ix
1 Monitoring the Proliferative Capacity of Cultured Animal Cells by Cell Cycle Analysis
1(16)
V. Leelavatcharamas
A. Nicholas Emery
Mohamed Al-Rubeai
2 Use of Flow Cytometry for Monitoring Antibody Productivity and Isolating High-Secreting Hybridoma Cells
17(22)
Katherine L. McKinney
Georges Belfort
3 Antibody Secretion Assays Using Gel Microdrops and Flow Cytometry
39(24)
James C. Weaver
John F. Dunne
Forest Gray
Kristina G. Lazzari
James B. Lin
4 High-Resolution Cell Cycle Analysis of Cell Cycle-Regulated Gene Expression
63(22)
Manfred Kubbies
Bernhard Goller
Guenter Giese
5 Stability of Monoclonal Antibody Production in Hybridoma Cell Culture
85(16)
Jose M. Coco Martin
E. Coen Beuvery
6 Flow Cytometric Studies of Osmotically Stressed and Sodium Butyrate-Treated Hybridoma Cells
101(20)
Steve Oh
Florence K. F. Chua
Mohamed Al-Rubeai
7 Flow Cytometric Analysis of Cells Obtained from Human Bone Marrow Cultures
121(26)
David Brott
Manfred R. Roller
Sue A. Rummel
Bernhard Palsson
8 Measurement of Intracellular pH During the Cultivation of Hybridoma Cells in Batch and Continuous Cultures
147(16)
Jean-Marc Engasser
Annie Marc
Marc Cherlet
Pierre Nabet
Patricia Franck
9 The Relationship Between Intracellular pH and Cell Cycle in Cultured Animal Cells Using SNARF-1 Indicator
163(14)
Jonathan P. Welsh
Mohamed Al-Rubeai
10 Assessment of Cell Viability in Mammalian Cell Lines
177(16)
Xavier Ronot
Sylvain Paillasson
Katherine A. Muirhead
11 Analysis of Apoptosis by Flow Cytometry
193(18)
Anne E. Milner
Hong Wang
Christopher D. Gregory
12 Monitoring Baculovirus Infection and Protein Expression in Insect Cells
211(14)
Anthanassia K. Kioukia
N. H. Simpson
Jatin D. Shah
A. Nicholas Emery
Mohamed Al-Rubeai
13 Use of Protein Distribution to Analyze Budding Yeast Population Structure and Cell Cycle Progression
225(16)
Danilo Porro
Lilia Alberghina
14 Cell Cycle Analysis of Microorganisms
241(16)
Kirsten Skarstad
Rolf Bemander
Sture Wold
Harald B. Steen
Erik Boye
15 Bacterial Characterization by Flow Cytometry
257(34)
Gerhard Nebe-von Caron
R. Andrew Badley
16 Assessment of Viability of Bacteria by Flow Cytometry
291(20)
Clive Edwards
17 Advances in the Flow Cytometric Characterization of Plant Cells and Tissues
311(16)
David W. Galbraith
Georgina M. Lambert
Index 327
Mohamed Al-Rubeai, A. Nicholas Emery
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