This invaluable resource presents practical biotechnological applications of flow cytometry techniques for the study of animal, plant, and microbial cells - explaining methodologies for sample preparation, staining, and analysis.
Generously illustrated and containing key bibliographic citations, Flow Cytometry Applications in Cell Culture is an excellent reference for biochemists, molecular and cell biologists, flow cytometrists, microbiologists, bioscientists, biochemical engineers, cytologists, immunologists, fermentation engineers, and graduate-level and continuing-education students in these disciplines.
Presents the latest research in flow cytometry for investigation and monitoring of cellular processes for biotechnological purposes, emphasizing the practical aspects of efficient operation of flow cytometric techniques. Topics include aspects of cell variability encountered in cell culture processes; quantitative analysis of heterogeneous populations; cell cycle analysis of animal cell cultures; and the use of flow cytometry to monitor and improve cell line selection. Discusses applications including analysis of baculovirus-infected cell culture, detecting cellular and subcellular changes in apoptosis, and antibody secretion assays using gel microdrops and flow cytometry. Of interest to microbiologists, biochemists, and biochemical engineers. Annotation copyright Book News, Inc. Portland, Or.