| 1 Introduction |
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1 | (22) |
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1.1 Agricultural Practices |
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2 | (1) |
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3 | (2) |
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5 | (1) |
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6 | (2) |
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8 | (6) |
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14 | (2) |
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16 | (2) |
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18 | (1) |
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18 | (5) |
| 2 The Basics |
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23 | (36) |
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23 | (10) |
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2.1.1 What Is Biotechnology? |
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23 | (3) |
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2.1.2 Genetic Material (DNA) |
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26 | (3) |
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29 | (3) |
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2.1.4 GMOs, GM Crops, and GM Foods |
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32 | (1) |
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2.1.4.1 Genetically Modified Organisms |
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32 | (1) |
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2.1.4.2 Genetically Modified Crops |
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32 | (1) |
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33 | (1) |
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2.2 Biotechnology-Past, Present, and Future |
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33 | (16) |
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2.2.1 Historical Developments in Biotechnology |
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33 | (3) |
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2.2.2 Biotechnology Timeline |
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36 | (9) |
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2.2.3 The Future of Biotechnology |
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45 | (4) |
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2.3 Types of Biotechnology |
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49 | (2) |
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49 | (1) |
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2.3.2 White Biotechnology |
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49 | (1) |
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2.3.3 Green Biotechnology |
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50 | (1) |
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50 | (1) |
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51 | (1) |
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51 | (1) |
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2.4 Position Statements on Biotechnology |
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51 | (2) |
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53 | (5) |
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58 | (1) |
| 3 Applications of Genetic Modification at the Laboratory and Greenhouse Levels |
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59 | (50) |
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3.1 Genetic Modification of Plants |
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59 | (1) |
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3.2 Nongenetic Engineering Techniques (Conventional Biotechnology) |
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60 | (9) |
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60 | (1) |
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3.2.2 Marker-Assisted Selection |
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60 | (1) |
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61 | (1) |
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3.2.4 Interspecies Crossing |
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62 | (1) |
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62 | (1) |
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3.2.6 Somatic Hybridization |
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63 | (1) |
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3.2.7 Somaclonal Variation |
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63 | (1) |
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64 | (1) |
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3.2.9 Plant TC and Micropropagation |
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65 | (4) |
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3.3 Unintended Effects from Conventional Biotechnology |
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69 | (1) |
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70 | (1) |
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70 | (30) |
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70 | (2) |
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3.4.2 From the Laboratory to the Greenhouse |
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72 | (1) |
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72 | (11) |
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3.4.3.1 Microbial Vectors |
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74 | (3) |
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77 | (1) |
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78 | (1) |
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3.4.3.4 Microprojectile Bombardment (Biolistics) |
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79 | (1) |
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80 | (1) |
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81 | (2) |
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3.4.3.7 Calcium Phosphate Precipitation |
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83 | (1) |
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83 | (1) |
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3.4.4 The Steps Followed in GE |
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83 | (5) |
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3.4.5 Methods for Detection, Identification, and Quantification of GMOs in Food and Feed |
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88 | (22) |
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88 | (2) |
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90 | (1) |
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3.4.5.3 GMO Testing Methods |
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91 | (5) |
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3.4.5.4 Validation and Standardization of Methods |
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96 | (1) |
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3.4.5.5 Reference Material, for GMO Testing |
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97 | (1) |
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3.4.5.6 The Future of GMO Testing |
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97 | (1) |
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3.4.5.7 ISO Standards for Detecting GMOs in Food |
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98 | (2) |
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3.5 Unintended Effects of GE |
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100 | (3) |
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103 | (3) |
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106 | (3) |
| 4 Applications of Genetic Modification at the Field and Commercial Levels |
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109 | (74) |
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109 | (1) |
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110 | (25) |
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4.2.1 Development of GM Crop Varieties with Improved Resistance to Herbicides Used for Weed Control |
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112 | (2) |
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4.2.2 Development of GM Crop Varieties with Improved Resistance to Pests |
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114 | (2) |
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4.2.3 Development of GM Crop Varieties with Improved Resistance to Bacterial, Fungal, and Viral Diseases |
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116 | (2) |
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4.2.3.1 Transgenic Crop Varieties Resistant to Bacterial Diseases |
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117 | (1) |
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4.2.3.2 Transgenic Crop Varieties Resistant to Fungal Diseases |
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117 | (1) |
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4.2.3.3 Transgenic Crop Varieties Resistant to Viral Diseases |
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117 | (1) |
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4.2.4 Development of GM Crop Varieties with Improved Resistance or Tolerance to Abiotic Factors |
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118 | (1) |
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4.2.5 Development of GM Crop Varieties with Improved Nutritional Quality Traits |
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119 | (2) |
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4.2.6 Weighing Risks and Benefits of Applying GE to Crops |
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121 | (1) |
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4.2.7 Production of Biofertilizers |
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122 | (4) |
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4.2.8 Global Status of GM Crops |
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126 | (4) |
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4.2.9 GM Crops/Foods in Developing Countries with Special Reference to Africa |
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130 | (5) |
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135 | (3) |
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4.3.1 Animal Genetic Modification Techniques |
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135 | (3) |
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4.3.1.1 Techniques That Do Not Use GE |
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135 | (1) |
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4.3.1.2 Techniques That Involve GE |
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136 | (2) |
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4.3.2 Biotechnology for Animal Feed Production |
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138 | (1) |
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138 | (32) |
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4.4.1 Introduction-Genetically Modified Foods |
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138 | (2) |
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4.4.2 Composition of GE Foods |
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140 | (1) |
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4.4.2.1 Intended Changes in Composition |
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140 | (1) |
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4.4.2.2 Unintended Changes in Composition |
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141 | (1) |
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4.4.3 Biotechnology in Food Processing and the Uses of GMOs in the Food Industry |
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141 | (15) |
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4.4.3.1 GM Crops (Products) Used as Food |
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141 | (4) |
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4.4.3.2 Food Companies Using GMO Ingredients |
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145 | (1) |
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4.4.3.3 Examples of GM Foods |
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145 | (4) |
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4.4.3.4 Processed Foods and Ingredients Based on GM Products |
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149 | (1) |
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4.4.3.5 Foods Produced from Animals Consuming GM Crops or Treated with Bovine Growth Hormone (BGH) |
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150 | (1) |
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4.4.3.6 Non-GMO Companies and Food Products |
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151 | (5) |
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4.4.3.7 Non-GMO Seed Companies |
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156 | (1) |
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4.4.3.8 Non-GMO Project Verified |
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156 | (1) |
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4.4.4 Biotechnology in Functional Foods and Nutraceuticals |
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156 | (10) |
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4.4.4.1 Production of Plant-Based Functional Foods |
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159 | (1) |
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4.4.4.2 Production of Animal-Based Functional Foods |
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159 | (1) |
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4.4.4.3 Application of Biotechnology for Production of Foods and Food Ingredients |
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160 | (6) |
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4.4.5 The Future of GM Food |
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166 | (4) |
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4.5 Biotechnology for Biofuel Production |
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170 | (2) |
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172 | (8) |
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180 | (3) |
| 5 Laws, Regulations, and Labeling for GM Foods |
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183 | (38) |
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183 | (2) |
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5.2 General Aspects of GM Food Regulations and Labeling |
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185 | (8) |
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5.2.1 The Principle of Substantial Equivalence |
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185 | (2) |
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187 | (1) |
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5.2.3 Issues with GM Food Labeling |
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187 | (2) |
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5.2.4 Pro- and Anti-Labeling Argtiments |
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189 | (2) |
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5.2.4.1 Pro-Labeling Arguments |
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189 | (1) |
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5.2.4.2 Anti-Labering Arguments |
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190 | (1) |
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5.2.5 Global GM Food labeling |
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191 | (2) |
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5.3 GM Foods Regulations and Labeling in Selected Countries |
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193 | (17) |
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193 | (2) |
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5.3.2 Australia-New Zealand |
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195 | (2) |
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197 | (2) |
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197 | (1) |
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198 | (1) |
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199 | (3) |
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202 | (2) |
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204 | (1) |
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205 | (5) |
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5.4 Consumer Perspectives on GM Food Labeling |
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210 | (1) |
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5.5 Intellectual Property (IP) and Patents |
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211 | (4) |
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215 | (5) |
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220 | (1) |
| 6 GM Foods or Not? The Controversy |
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221 | (74) |
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221 | (5) |
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6.2 Issues of Concern and Controversy |
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226 | (6) |
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6.2.1 Concerns about Food Safety and Human Health |
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226 | (2) |
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6.2.2 Environmental Concerns |
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228 | (2) |
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230 | (1) |
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6.2.4 Regulatory and Legal Concerns |
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230 | (1) |
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230 | (1) |
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6.2.6 Animal Health and Welfare |
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231 | (1) |
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231 | (1) |
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6.2.8 Concerns about Bias in Scientific Publishing |
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232 | (1) |
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232 | (19) |
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6.3.1 Who Are the Proponents? |
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232 | (9) |
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6.3.1.1 International Bodies and Governments |
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232 | (1) |
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6.3.1.2 Biotech, Agrochemical, and Associated Companies |
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233 | (3) |
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6.3.1.3 Pro-GM Lobby Groups |
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236 | (5) |
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6.3.1.4 Global Network of Pro-Corporate Activists |
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241 | (1) |
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6.3.2 Arguments in Favor of GM Foods |
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241 | (10) |
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6.3.2.1 Benefits for Farmers |
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242 | (1) |
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6.3.2.2 Benefits for Consumers |
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243 | (3) |
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6.3.2.3 Benefits for the Environment |
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246 | (3) |
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6.3.2.4 Benefits for the Economy |
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249 | (1) |
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6.3.2.5 Other Points in Favor of GM Foods |
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250 | (1) |
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251 | (26) |
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251 | (1) |
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6.4.2 Who Are the Opponents? |
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252 | (8) |
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252 | (4) |
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256 | (2) |
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6.4.2.3 Canada, Australia, and New Zealand |
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258 | (1) |
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258 | (2) |
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6.4.2.5 Central and South America |
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260 | (1) |
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260 | (1) |
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6.4.3 Popular Protests against GMOs and GM Foods |
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260 | (1) |
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6.4.4 Vandalism and Threats by Anti-GMO Activists |
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261 | (1) |
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6.4.5 Arguments against GM Foods |
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262 | (15) |
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6.4.5.1 Better Alternatives to GM Technology |
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263 | (1) |
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6.4.5.2 Hazards and Risks of GM |
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264 | (6) |
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6.4.5.3 Impact on the Environment, the Ecosystem, and Farming |
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270 | (5) |
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6.4.5.4 Ethical and Moral Objections |
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275 | (1) |
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6.4.5.5 GM Technology Creates Socioeconomic and Political Threats |
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275 | (2) |
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6.5 And the Debate Continues! |
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277 | (1) |
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278 | (7) |
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285 | (10) |
| 7 Consumer Issues |
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295 | (28) |
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295 | (3) |
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298 | (2) |
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7.3 Studies on Consumers' Perceptions, Attitudes, and Preferences for GM Foods |
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300 | (12) |
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7.4 Impact of Moving from Including GM Components to Non-GM Components on Food Sales |
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312 | (1) |
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313 | (1) |
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314 | (5) |
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319 | (4) |
| Glossary |
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323 | (60) |
| Index |
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383 | |