Muutke küpsiste eelistusi

E-raamat: Insect Pathogens: Molecular Approaches and Techniques [CABI e-raamatud]

Edited by (ARO, Volcani Center, Israel), Edited by (University of Arizona, USA), Edited by , Edited by (USDA-ARS, USA)
  • Formaat: 440 pages
  • Ilmumisaeg: 31-Jan-2009
  • Kirjastus: CABI Publishing
  • ISBN-13: 9781845934781
Teised raamatud teemal:
  • CABI e-raamatud
  • Hind: 102,00 €*
  • * hind, mis tagab piiramatu üheaegsete kasutajate arvuga ligipääsu piiramatuks ajaks
Insect Pathogens: Molecular Approaches and TechniquesSuurem pilt
  • Formaat: 440 pages
  • Ilmumisaeg: 31-Jan-2009
  • Kirjastus: CABI Publishing
  • ISBN-13: 9781845934781
Teised raamatud teemal:
Wiley OnlineRohkem infot CABI e-raamatute kohta
Investigation of insect pathogens is vital to the understanding of biocontrol and insect management within an ecosystem. Faster and more accurate methods of identification and diagnosis have become possible with the implementation of molecular techniques. Advances in genomics and genetic engineering make this manual the most up to date handbook on insect pathogens, with the latest information on methods used to unravel the genomes of pathogens. The book is divided into four sections covering: Identification and Diagnostics, Evolutionary Relationships and Population Genetics, Genomics, and Genetic Engineering. It is essential reading for those studying and researching at the forefront of molecular science and biological management.
Contributors xi
Preface xiii
Glossary of Terms xv
PART I IDENTIFICATION AND DIAGNOSTICS 1
1 Molecular Approaches to Virus Characterization and Detection
3
M.A. Erlandson and D.A. Theilrnann
1.1. Introduction
4
1.2. Current Virus Taxonomy and Classification
4
1.3. Preliminary Approaches to Virus Identification
13
1.4. Methodology for Virus Isolation and Fractionation (Nucleic Acid and Protein Purification Techniques)
13
1.5. Biochemical/Molecular Approaches to Virus Identification and Diagnosis
14
1.6. PCR and Virus-specific Primer Development
21
1.7. Conclusions
26
2 Molecular Approaches and Techniques for the Study of Entomopathogenic Bacteria
32
N. Boemare and P. Tailliez
2.1. Introduction
32
2.2. Classification of Entomopathogenic Bacteria
33
2.3. Conclusions
44
3 Molecular Methods for Identification and Diagnosis of Fungi
50
L.A. Castrillo and R.A. Humber
3.1. Introduction
50
3.2. General Considerations
51
3.3. Genetic Fingerprinting
54
3.4. DNA Sequencing
64
3.5. Diagnosis and Detection
65
3.6. Conclusions and Future Prospects
66
4 Molecular Approaches and the Taxonomy of Insect-parasitic and Pathogenic Nematodes
71
S.P. Stock
4.1. Introduction
71
4.2. Nematode Diagnosis and the Barcode System
72
4.3. A Review of Molecular Approaches Considered for Insect-parasitic and Pathogenic Nematode Taxonomy
73
4.4. Techniques Considered for Obtaining DNA Sequences
75
4.5. Sequencing
93
4.6. Sequence Manipulation and Analysis
93
4.7. Conclusions
94
5 Identification and Diagnostics of Entomopathogenic Protozoa
101
M. Obornik
5.1. Introduction
101
5.2. Molecular Identification of Species and Strains
102
5.3. Conclusion and Future Perspectives
123
PART II EVOLUTIONARY RELATIONSHIPS AND POPULATION GENETICS 129
6 Phylogenetic Studies with Entomopathogenic Bacteria with Special Emphasis on Symbionts of Entomopathogenic Nematodes
131
P. Tailliez and N. Boemare
6.1. Introduction
131
6.2. Genes Considered
132
6.3. Conclusions and Perspectives
141
7 Molecular Systematics of Entomopathogenic Fungi
145
S.A. Rohner
7.1. Introduction
145
7.2. Molecular Phylogenies of Fungi and the Origins of Entomopathogens
146
7.3. Species Recognition
154
7.4. Species-level Phylogenies of Entomopathogenic Fungi
157
7.5. Conclusions
158
8 Phylogenetics and Population Genetics of Entomopathogenic and Insect-parasitic Nematodes
166
S.M. Peat, B.C. Hyman and B.J. Adams
8.1. Introduction
166
8.2. Phylogenetics
167
8.3. Population Genetics
168
8.4. DNA Bar Coding
168
8.5. DNA Markers Considered for Phylogenetic and 169 Population Genetics Studies
8.6. Methodology
175
8.7. Co-phylogenesis and Cospeciation
182
8.8. Population Genetics Methods
184
PART III HOST–PATHOGEN INTERACTIONS 193
9 Host–Virus Interactions
195
J.P. Burand, M. Nakai and I. Smith
9.1. Introduction
195
9.2. Use of Viruses as Insect Pest Control Agents
196
9.3. Development of Baculoviruses for Foreign Gene Expression
197
9.4. Insect Defences Against Viruses
201
9.5. Baculovirus Pathogenesis
202
9.6. Baculovirus Host Range
207
9.7. Unclassified DNA Viruses
210
9.8. Mechanisms of Insect Virus Persistence
212
9.9. Conclusions and Future Prospects
214
10 Insect–Protozoa–Bacteria Associations: a Model System for Investigating Host–Parasite Interactions
223
B.L. Weiss, G.M. Attarclo and S. Aksoy
10.1. Introduction
223
10.2. Trypanosomatid Protozoa and Tsetse Flies
224
10.3. Molecular Approaches and Their Application to Study Insect Host immune Responses
226
10.4. Tsetse Ern' isymbionts
228
10.5. Control if Insect-borne Diseases
230
10.6. Current and Future Work
234
10.7. Conclusions
236
11 Methods in Investigating Nematode–Bacterium–Insect Symbiosis
241
H. Goodrich-Blair; D.J. Clarke, P.S. Grewal and T.A. Ciche
11.1. Introduction
242
11.2. Molecular Tools to Study Entomopathogenic Nematodes
244
11.3. Basic Molecular Tools for the Study of Entomopathogcnic Bacteria
250
11.4. Techniques to Investigate Bacteria–Nematode Mutualism
258
11.5. Techniques in Studying EPB Virulence
261
11.6. Conclusions and Future Prospects
265
PART IV GENOMICS AND GENETIC ENGINEERING 2 73
12 Genetic Engineering of Bacteria to Improve Efficacy Using the Insecticidal Proteins of Bacillus Species
275
H.-W Park and B.A. Federici
12.1. Introduction
275
12.2. Basic Biology of Bacillus thuringiensis
276
12.3. Insecticidal Proteins of Bacillus thuringiensis
277
12.4. Genetic Factors Regulating Insecticidal Proteins
280
12.5. Construction of Recombinant Bacteria
281
12.6. Conclusions and Future Prospects
298
13 Genomic Analysis of the Symbiotic and Entomopathogenic Photorhabdus Bacteria
306
S. Gaudriaidt and E. Duchand
13.1. Introduction
307
13.2. Sequencing and Annotation of Plwtorlialnlus Genomes
308
13.3. Main Features of the P haninescens Genome
310
13.4. Analogical Post-genomic Analysis
313
13.5. Post-genomic Analysis by a 'Blind' Approach
316
13.6. Conclusions and Future Perspectives
325
14 Genomics of Entomopathogenic Viruses
329
J. Slack, Z. Li, S. Escasa, D. Doucet, T. Ladd, G. Quan and B. Arif
14.1. Introduction
329
14.2. General Concepts
310
14.3. Analyses of DNA and Protein Sequences
335
14.4. Genetic Modification of Baculoviruses
339
14.5. Conclusions
342
15 Genomics and Genetic Improvement of Entomopathogenic Nematodes
346
H. Kollai
15.1. Introduction
346
15.2. Genomic Sequencing and Ilioinformaties
347
15.3. Functional Genomics: Towards Deciphering of Genomics and FSTs Sequences
353
15.4. Genetic Improvement
357
15.5. Conclusion and Future Prospects
358
16 Entomopathonic Fungi and the Genomics Era
365
R.J. St Leger and C. Wang
16.1. Introduction
366
16.2. Procedures for Isolating Pathogen DNA
368
16.3. Procedures for Isolating Pathogen RNA
370
16.4. Pulsed Field Gel Electrophoresis
371
16.5. Construction of Cloning and Expression Vector Components and Markers
372
16.6. Transformation Systems
376
16.7. Gene Cloning Strategies
379
16.8. Analysing Differential Gene Expression
389
16.9. EST Screening
392
16.10. Microarray Analysis
392
16.11. Targeted Gene Mutagenesis
394
16.12. RNA Interference
395
16.13. Conclusions
395
Index 401
Goes by the name "S. Patricia Stock" Itamar Glazer is Researcher at the Agricultural Research Organization (ARO), Volcani Center, Israel.
Püsilink: https://www.kriso.ee/db/9781845934781_pe.html
Märksõnad: