There is increasing recognition of the diversity and biological importance of lipids. Lipid modifications of other biological molecules are now also the subject of intense research activity. This 'user-friendly' introduction describes the techniques curre
Reviews
"Lipid Analysis is an inexpensive and well-produced book, with many clear figures and line drawings to illustrate separations." Trends in Food Science and Technology November 1996 "It is a thorough-going account at a research level (which is) extremely we
Abbreviations -- Preface --
1. Introduction --
2. Basic Techniques -- Extraction methods -- Lipid binding in tissues -- Solvents for lipid extraction -- Some precautions -- Storage of lipid extracts -- Saponification -- Chromatographic procedures -- General considerations -- Partition chromatography -- Adsorption chromatography -- Ion exchange chromatography -- Complexing chromatography -- Spectrometric methods -- UV-visible spectrometry -- IR spectroscopy -- NMR spectroscopy -- Mass spectrometry -- Radioisotopic methods -- Choice of radioisotope -- Autoradiography -- Scintillation counting -- Gas ionization detection -- Gamma counting -- Radioisotopes in lipid studies -- RIA and related techniques -- Immunochemical methods -- Antibodies to lipids -- Hemagglutination in detection and assay -- Immunostaining and TLC -- Immunoradiometric assays -- Enzyme-linked immunosorbent assays -- Analytical methods involving enzymes -- Phospholipases -- Stereospecific analysis of triacylglycerol -- Enzymes in quantifying lipids -- Presentation of lipids in aqueous systems -- Care and safety during the analysis of lipids in biological materials -- General considerations -- Chemical hazards -- Fire hazards -- Radioisotope hazards -- Biological hazards -- Other hazards -- Personal preparedness and protection -- References --
3. Hydrocarbons -- Biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- References --
4. Alcohols, Phenols, Aldehydes, Ketones and Quinones -- Alcohols and phenols -- General and biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- Aldehydes and ketones -- General and biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- Quinones -- General and biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- References --
5. Fatty Acids and Prostaglandins -- Fatty acids -- Types of fatty acids found naturally -- Separation methods for fatty acids -- GLC of fatty acids -- HPLC of fatty acids -- Prostaglandins and leukotrienes (eicosanoids) -- Analysis of eicosanoids -- References --
6. Esters -- Acylglycerols -- Separation of acylglycerols -- Analysis of acylglycerols -- Fatty acids of acylglycerols -- Wax esters -- Alkylglycerols -- Analytical methods for alkylglycerols -- References --
7. Phospholipids, Sulfolipids and Related Compounds -- Phospholipids with amino-groups -- Hydrolytic procedures in analysis -- Estimation of phosphate -- Separation of phospholipids -- Phosphoinositides -- Phosphoinositide and inositol phosphate metabolism -- Separation of the phosphoinositides -- Receptor-linked phosphoinositide changes -- Separation and estimation of inositol phosphates -- Estimation of diacylglycerol -- Phosphatidylinositol glycans -- Detection of PI glycans -- Phosphonolipids -- Separation and analysis of phosphonolipids -- Alkylether phospholipids -- Analysis of alkylether phospholipids -- Sulfolipids -- Lipid sulfates -- Sulfonolipids -- Analytical methods -- References --
8. Glycolipids -- Glycosphingolipids -- Biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- Polyisoprenyl phosphosugars -- Biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- Glycosylglycerides and related compounds -- Biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- Phosphoglycolipids, lipoteichoic acids and related compounds -- Biological significance -- Structures -- Detection, isolation, preparation and quantitation -- References --
9. Lipoproteins -- Plasma lipoproteins -- Structures and biological significance -- Detection -- Isolation and purification -- Quantitation -- Fatty acyl proteins and prenyl proteins -- Biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- References -- Appendices -- Appendix A: Glossary -- Appendix B: Suppliers -- Appendix C: Further reading -- Index.
Department of Biochemistry, Medical School, Queen's Medical Centre, Nottingham NG7 2UH, UK. Department of Biochemistry, Medical School, Queen's Medical Centre, Nottingham NG7 2UH, UK.
