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E-raamat: Optics in Instruments: Applications in Biology and Medicine [Wiley Online]

Edited by (Université Jean Monnet, Saint-Etienne, France)
  • Formaat: 256 pages
  • Sari: ISTE
  • Ilmumisaeg: 16-Apr-2013
  • Kirjastus: ISTE Ltd and John Wiley & Sons Inc
  • ISBN-10: 1118574389
  • ISBN-13: 9781118574386
Teised raamatud teemal:
  • Wiley Online
  • Hind: 174,45 €*
  • * hind, mis tagab piiramatu üheaegsete kasutajate arvuga ligipääsu piiramatuks ajaks
Optics in Instruments: Applications in Biology and MedicineSuurem pilt
  • Formaat: 256 pages
  • Sari: ISTE
  • Ilmumisaeg: 16-Apr-2013
  • Kirjastus: ISTE Ltd and John Wiley & Sons Inc
  • ISBN-10: 1118574389
  • ISBN-13: 9781118574386
Teised raamatud teemal:
Wiley Online e-raamatudRohkem infot Wiley Online kohta
Raamatu kodulehekülg: https://onlinelibrary.wiley.com/doi/book/10.1002/9781118574386
Optics is a science which covers a very large domain and is experiencing indisputable growth. It has enabled the development of a considerable number of instruments, the optical component or methodology of which is often the essential part of portent systems. This book sets out show how optical physical phenomena such as lasers the basis of instruments of measurement are involved in the fields of biology and medicine. Optics in Instruments: Applications in Biology and Medicine details instruments and measurement systems using optical methods in the visible and near-infrared, as well as their applications in biology and medicine, through looking at confocal laser scanning microscopy, the basis of instruments performing in biological and medical analysis today, and flow cytometry, an instrument which measures at high speed the parameters of a cell passing in front of one or more laser beams. The authors also discuss optical coherence tomography (OCT), which is an optical imaging technique using non-contact infrared light, the therapeutic applications of lasers, where they are used for analysis and care, and the major contributions of plasmon propagation in the field of life sciences through instrumental developments, focusing on propagating surface plasmons (PSP) and localized plasmons (LP).

Contents:

1. Confocal Laser Scanning Microscopy, Thomas Olivier and Baptiste Moine. 2. Flow Cytometry (FCM) Measurement of Cells in Suspension, Odile Sabido. 3. Optical Coherence Tomography, Claude Boccara and Arnaud Dubois. 4. Therapeutic Applications of Lasers, Geneviève Bourg-Heckly and Serge Mordon. 5. Plasmonics, Emmanuel Fort.





About the Authors

Jean-Pierre Goure is Emeritus Professor of optics at Jean Monnet University in Saint-Etienne, France, and was previously director of the UMR 5516 laboratory linked with CNRS. He is the author of more than 100 publications in various fields, such as spectroscopy, instrumentation, sensors, optical fiber and optical communications. He was also previously deputy director in engineering science at CNRS and a member of several scientific associations such as the French Optical Society and the European Optical Society.
Preface ix
Introduction xiii
Chapter 1 Confocal Laser Scanning Microscopy
1(78)
Thomas Olivier
Baptiste Moine
1.1 Introduction
1(5)
1.1.1 Context and framework of chapter
1(2)
1.1.2 From wide-field microscopy to confocal microscopy
3(3)
1.2 Principle and implementation
6(34)
1.2.1 General principle
7(2)
1.2.2 Axial and lateral resolution in confocal microscopy
9(12)
1.2.3 Some notions of fluorescence
21(4)
1.2.4 Main elements of a confocal scanning laser microscope
25(15)
1.3 Applications in biology, potential and limitations
40(22)
1.3.1 Basic elements of biology for the neophyte
41(2)
1.3.2 Fluorescent labeling
43(3)
1.3.3 Practical implementation of confocal microscopy
46(16)
1.4 Related and derived techniques
62(12)
1.4.1 Advanced contrast modes: FRAP, FLIP, FLIM, FRET, etc.
62(4)
1.4.2 The contribution of nonlinear contrast modes
66(6)
1.4.3 Recent major advances: overcoming the diffraction limit
72(2)
1.5 Bibliography
74(5)
Chapter 2 Flow Cytometry (FCM) Measurement of Cells in Suspension
79(22)
Odile Sabido
2.1 History of FCM
79(1)
2.2 Components of the cytometer: fluidics, optics and signal processing
80(3)
2.2.1 Fluidics
81(1)
2.2.2 Optics
81(2)
2.2.3 Signal processing
83(1)
2.3 Experimentation strategy
83(4)
2.3.1 Visualizations of the spectra
84(1)
2.3.2 Compensation of fluorescences
84(1)
2.3.3 Checking the optical bench
84(1)
2.3.4 Presentation of parameters A/H/W
85(1)
2.3.5 Graphical presentation
85(2)
2.4 Types of platform for FCM
87(1)
2.4.1 Clinical platform
87(1)
2.4.2 Research platform
87(1)
2.5 Principle of cell sorting
88(2)
2.6 Analyzed parameters
90(3)
2.6.1 Light scattering
90(1)
2.6.2 Fluorochromes
90(3)
2.7 Applications in biology
93(2)
2.7.1 Clinical
93(1)
2.7.2 Research
93(1)
2.7.3 Environment
94(1)
2.7.4 Plant biology
94(1)
2.7.5 Industrial microbiology
94(1)
2.8 Complementarities of the FCM with the other cytometries, confocal and dynamic
95(1)
2.9 Cytometry on beads, LUMINEX™type
95(1)
2.10 Scientific societies
96(1)
2.11 Websites to visit
96(1)
2.12 Bibliography
97(2)
2.13 Reference books
99(2)
Chapter 3 Optical Coherence Tomography
101(24)
Claude Boccara
Arnaud Dubois
3.1 Introduction
101(1)
3.2 Principles of OCT
102(2)
3.3 Frequency-domain OCT
104(2)
3.4 Spatial resolution
106(1)
3.5 Applications of OCT
107(2)
3.5.1 Ophtalmology
107(1)
3.5.2 Internal medicine
107(1)
3.5.3 Other fields of application
108(1)
3.6 Extensions of OCT
109(1)
3.7 Full-field OCT
110(9)
3.7.1 Principle
110(1)
3.7.2 Spatial resolution
111(2)
3.7.3 Dynamics and sensitivity
113(1)
3.7.4 Operating speed
113(1)
3.7.5 Applications
114(5)
3.8 Conclusion
119(1)
3.9 Bibliography
119(6)
Chapter 4 Therapeutic Applications of Lasers
125(54)
Genevieve Bourg-Heckly
Serge Mordon
4.1 Introduction
125(2)
4.2 Interaction of light with biological tissues
127(28)
4.2.1 Optical parameters characterizing light radiation
127(4)
4.2.2 The three types of interaction between a light beam and a biological tissue
131(20)
4.2.3 Penetration of light in biological tissues
151(4)
4.3 Therapeutic effects of lasers
155(20)
4.3.1 Thermal effect
156(11)
4.3.2 Photoablative effect
167(1)
4.3.3 Photochemical or photodynamic effect
168(6)
4.3.4 The electromechanical effect
174(1)
4.4 Conclusion
175(1)
4.5 For more information
175(1)
4.6 Bibliography
176(3)
Chapter 5 Plasmonics
179(38)
Emmanuel Fort
5.1 Propagating surface plasmons
180(21)
5.1.1 Theoretical reminders and definitions
180(5)
5.1.2 Surface plasmon resonance sensors
185(4)
5.1.3 Units and sensitivity of SPR sensors
189(1)
5.1.4 Other SPR configurations
190(1)
5.1.5 SPR imaging
191(3)
5.1.6 Surface plasmons coupled fluorescence
194(7)
5.2 Localized surface plasmons
201(9)
5.2.1 Theoretical reminders
201(2)
5.2.2 Detection of plasmonic nanoprobes
203(7)
5.3 Conclusion
210(1)
5.4 Bibliography
211(6)
List of Authors 217(2)
Index 219
Jean Pierre Goure is Emeritus Professor of optics at Jean Monnet University in Saint-Etienne, France, and was previously director of the UMR 5516 laboratory linked with CNRS. He is the author of more than 100 publications in various fields, such as spectroscopy, instrumentation, sensors, optical fiber and optical communications. He was also previously deputy director in engineering science at CNRS and a member of several scientific associations such as the French Optical Society and the European Optical Society.
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