Muutke küpsiste eelistusi

E-raamat: Problem Solving in Enzyme Biocatalysis [Wiley Online]

(School of Biochemical Engineering, Universidad Católica de Valparaiso, Chile), (School of Biochemical Engineering, Universidad Católica de Valparaiso, Chile), (School of Biochemical Engineering, Universidad Católica de Valparaiso, Chile)
  • Formaat: 344 pages
  • Ilmumisaeg: 13-Dec-2013
  • Kirjastus: John Wiley & Sons Inc
  • ISBN-10: 1118341740
  • ISBN-13: 9781118341742
Teised raamatud teemal:
  • Wiley Online
  • Hind: 120,53 €*
  • * hind, mis tagab piiramatu üheaegsete kasutajate arvuga ligipääsu piiramatuks ajaks
Problem Solving in Enzyme BiocatalysisSuurem pilt
  • Formaat: 344 pages
  • Ilmumisaeg: 13-Dec-2013
  • Kirjastus: John Wiley & Sons Inc
  • ISBN-10: 1118341740
  • ISBN-13: 9781118341742
Teised raamatud teemal:
Wiley Online e-raamatudRohkem infot Wiley Online kohta
Raamatu kodulehekülg: https://onlinelibrary.wiley.com/doi/book/10.1002/9781118341742
"The only book to approach enzyme kinetics with a problem-solving focus, for practical applications in the food, pharmaceutical, and fine chemistry industry"--

This textbook for chemical and biochemical engineering students could also serve as a handbook for biochemists, chemists, and biologists dealing with biocatalysis processes. Each chapter begins with an abridged account of a particular topic, then presents sample exercises in the form of solved problems that illustrate resolution procedures and the main concepts underlying them. Supplementary problems are for students to solve, but solutions are provided. The topics are facts and figures in enzyme biocatalysis; enzyme kinetics in a homogeneous system and a heterogeneous system; enzyme reactor design and operation under ideal conditions, under mass-transfer limitations, and under biocatalyst inactivation; and optimizing enzyme reactor operation. An appendix reviews mathematical methods. Annotation ©2014 Ringgold, Inc., Portland, OR (protoview.com)

Enzyme biocatalysis is a fast-growing area in process biotechnology that has expanded from the traditional fields of foods, detergents, and leather applications to more sophisticated uses in the pharmaceutical and fine-chemicals sectors and environmental management. Conventional applications of industrial enzymes are expected to grow, with major opportunities in the detergent and animal feed sectors, and new uses in biofuel production and human and animal therapy.

In order to design more efficient enzyme reactors and evaluate performance properly, sound mathematical expressions must be developed which consider enzyme kinetics, material balances, and eventual mass transfer limitations. With a focus on problem solving, each chapter provides abridged coverage of the subject, followed by a number of solved problems illustrating resolution procedures and the main concepts underlying them, plus supplementary questions and answers.

Based on more than 50 years of teaching experience, Problem Solving in Enzyme Biocatalysis is a unique reference for students of chemical and biochemical engineering, as well as biochemists and chemists dealing with bioprocesses.


Contains: Enzyme properties and applications; enzyme kinetics; enzyme reactor design and operation 146 worked problems and solutions in enzyme biocatalysis.

Preface ix
Nomenclature xi
Epsilon Software Information xxi
Acknowledgement xxv
1 Facts and Figures in Enzyme Biocatalysis
1(10)
1.1 Introduction
1(2)
1.1.1 Enzyme Properties
1(1)
1.1.2 Enzyme Applications
2(1)
1.2 Enzymes as Process Catalysts
3(2)
1.3 Evolution of Enzyme Biocatalysis: From Hydrolysis to Synthesis
5(1)
1.4 The Enzyme Market: Figures and Outlook
6(5)
References
7(4)
2 Enzyme Kinetics in a Homogeneous System
11(76)
2.1 Introduction
11(3)
2.1.1 Concept and Determination of Enzyme Activity
11(2)
2.1.2 Definition of a Unit of Activity
13(1)
2.1.3 Measurement of Enzyme Activity
13(1)
2.2 Theory of Enzyme Kinetics
14(3)
2.3 Single-Substrate Reactions
17(2)
2.3.1 Kinetics of Enzyme Inhibition
18(1)
2.4 Multiple-Substrate Reactions
19(2)
2.4.1 Reaction Mechanisms
19(1)
2.4.2 Kinetics of Enzyme Reactions with Two Substrates
20(1)
2.5 Multiple-Enzyme Reactions
21(1)
2.6 Determination of Kinetic Parameters
22(2)
2.7 Effects of Operational Variables on Enzyme Kinetics
24(63)
2.7.1 Effects of pH
25(1)
2.7.2 Effects of Temperature
26(3)
Solved Problems
29(43)
Supplementary Problems
72(12)
References
84(3)
3 Enzyme Kinetics in a Heterogeneous System
87(54)
3.1 Introduction
87(1)
3.2 Immobilization of Enzymes
87(5)
3.2.1 Immobilization on Solid Supports (Carrier-Bound Systems)
88(1)
3.2.2 Immobilization by Containment
89(1)
3.2.3 Immobilization in Carrier-Free Systems
89(1)
3.2.4 Parameters of Enzyme Immobilization
90(1)
3.2.5 Optimization of Enzyme Immobilization
91(1)
3.3 Mass-Transfer Limitations in Enzyme Catalysis
92(10)
3.3.1 Partition Effects
93(1)
3.3.2 External Diffusional Restrictions in Impervious Biocatalysts
94(3)
3.3.3 Internal Diffusional Restrictions in Porous Biocatalysts
97(5)
3.4 Determination of Intrinsic Kinetic and Mass-Transfer Parameters
102(39)
3.4.1 EDR
102(2)
3.4.2 IDR
104(1)
Solved Problems
105(22)
Supplementary Problems
127(11)
References
138(3)
4 Enzyme Reactor Design and Operation under Ideal Conditions
141(40)
4.1 Modes of Operation and Reactor Configurations
141(1)
4.2 Definition of Ideal Conditions
142(1)
4.3 Strategy for Reactor Design and Performance Evaluation
143(1)
4.4 Mathematical Models for Enzyme Kinetics, Modes of Operation, and Reactor Configurations under Ideal Conditions
143(38)
4.4.1 Batch Enzyme Reactor
144(4)
4.4.2 Continuous Enzyme Reactors
148(9)
Solved Problems
157(17)
Supplementary Problems
174(5)
References
179(2)
5 Enzyme Reactor Design and Operation under Mass-Transfer Limitations
181(22)
5.1 Sequential Batch and Continuously Operated Reactors with Immobilized Enzymes
182(1)
5.2 Mathematical Models for Enzyme Kinetics, Modes of Operation, and Reactor Configurations under Mass-Transfer Limitations
183(20)
Solved Problems
185(13)
Supplementary Problems
198(5)
6 Enzyme Reactor Design and Operation under Biocatalyst Inactivation
203(40)
6.1 Mechanistically Based Mathematical Models of Enzyme Inactivation
203(2)
6.2 Effect of Catalytic Modulators on Enzyme Inactivation
205(1)
6.3 Mathematical Models for Different Enzyme Kinetics, Modes of Operation, and Reactor Configurations under Biocatalyst Inactivation
206(6)
6.3.1 Nonmodulated Enzyme Inactivation
206(3)
6.3.2 Modulated Enzyme Inactivation
209(3)
6.4 Mathematical Models for Enzyme Kinetics, Modes of Operation, and Reactor Configurations under Simultaneous Mass-Transfer Limitations and Enzyme Inactivation
212(1)
6.5 Strategies for Reactor Operation under Biocatalyst Inactivation
213(30)
Solved Problems
215(18)
Supplementary Problems
233(7)
References
240(3)
7 Optimization of Enzyme Reactor Operation
243(34)
7.1 Strategy for the Optimization of Enzyme Reactor Performance
244(3)
7.1.1 Objective Function
244(2)
7.1.2 Variables for Optimization of Enzyme Reactor Performance
246(1)
7.1.3 Determination of Optimum Temperature
247(1)
7.2 Mathematical Programming for Static Optimization
247(1)
7.3 Dynamic Programming
248(1)
7.4 Statistical Optimization by Surface Response Methodology
249(28)
7.4.1 Assessing the Quality of SRM and its Parameters
251(1)
7.4.2 Process Optimization by SRM
252(2)
Solved Problems
254(18)
Supplementary Problems
272(3)
References
275(2)
Appendix A Mathematical Methods
277(34)
A.1 Newton's Method
277(3)
A.2 Curve Fitting by Least Squares
280(16)
A.2.1 Linear Regression
280(6)
A.2.2 Nonlinear Regression
286(10)
A.3 Solving Ordinary Differential Equations
296(6)
A.3.1 Solving First-Order Ordinary Differential Equations by the Separation of Variables
296(1)
A.3.2 Solving First-Order Ordinary Differential Equations Using an Integration Factor
297(1)
A.3.3 Solving Second- and Higher-Order Linear Homogeneous Differential Equations with Constant Coefficients Using their Characteristic Equations
298(3)
A.3.4 Solving Second- and Higher-Order Linear Homogeneous Differential Equations with Variable Coefficients
301(1)
A.4 Numerical Methods for Solving Differential Equations
302(9)
A.4.1 The Euler Method
302(1)
A.4.2 The Fourth-Order Runge--Kutta Method
303(1)
A.4.3 The Finite-Difference Method
303(7)
References
310(1)
Index 311
Andrés Illanes is Professor in the School of Biochemical Engineering at Pontificia Universidad Católica de Valparaíso, Chile. He has been researching enzyme biocatalysis since the 1970s, having done research in the main topics related to enzyme technology, and taught many courses at the undergraduate, M.Sc and Ph.D level in the subject both in Chile and abroad. He has authored over 80 ISI journal publications, several book chapters and three books on this topic, the latest with Springer 2008 Enzyme Biocatalysis: Principles and Applications.

Lorena Wilson is Associate Professor at the School of Biochemical Engineering, Pontificia Universidad Católica de Valparaíso. She has worked on enzyme biocatalysis since her time as an undergraduate and done research in aspects related mostly to biocatalyst engineering and enzyme reactor performance. She has more than ten years teaching experience focused mostly on the subject of enzyme biocatalysis. She is a Biochemical Engineer with a PhD from the Universidad Autónoma de Madrid, Spain. Dr Wilson is also author of more than 40 ISI publications in high ranked journals and several book chapters.

Carlos Vera works in the School of Biochemical Engineering at Pontificia Universidad Católica de Valparaíso, Chile.
Püsilink: https://www.kriso.ee/db/9781118341742_pe.html
Märksõnad: