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E-raamat: Spectroscopic Membrane Probes: Volume 2 [Taylor & Francis e-raamat]

  • Formaat: 216 pages
  • Ilmumisaeg: 29-Mar-2018
  • Kirjastus: CRC Press
  • ISBN-13: 9781351076791
  • Taylor & Francis e-raamat
  • Hind: 133,87 €*
  • * hind, mis tagab piiramatu üheaegsete kasutajate arvuga ligipääsu piiramatuks ajaks
  • Tavahind: 191,24 €
  • Säästad 30%
  • Formaat: 216 pages
  • Ilmumisaeg: 29-Mar-2018
  • Kirjastus: CRC Press
  • ISBN-13: 9781351076791
The optical spectra of molecular membrane probes can be interpreted terms of the structure, dynamics, and the physiological state of the membrane. The general picture we have of membranes and of the properties of the proteins imbedded in them, has, arguably, emerged directly from probe studies over the last 20 years. This work is designed to make these techniques accessible to a broad audience of cell biologist. The techniques discussed revolve primarily around the fluorescence of membrane probes, but applications of light absorption and Raman scattering are included. In addition to reviews of the major applications, most chapters include information on required apparatus, experimental design, data analysis, and potential pitfalls.

The optical spectra of molecular membrane probes can be interpreted terms of the structure, dynamics, and the physiological state of the membrane. The general picture we have of membranes and of the properties of the proteins imbedded in them, has, arguably, emerged directly from probe studies over the last 20 years.
1. Introduction
2. Membrane Fluidity
3. Studies of Membrane dynamics
4. Structure of Membrane Protein
5. Structural Mapping of Membrane Associated Proteins
6. Fluorescent Assays for Membrane Fusion
7. Use of Membrane
8. Associated Fluorescence Probes to Monitor Fusion of Bilayer Vesicles
9. Probing the Lateral Organization and Dynamics of Membranes
10. Cell Surface Clustering and Mobility of the Liganded LDL Receptor Measured by Digital Video
11. Total Internal Reflection Fluorescenc
12. Near-Fielded Imaging of Fluorescence
13. Fluorescence Probes of Anion Transport Inhibition in the Human Red Cell Membrane
14. How to Choose a Potentiometric Membrane Probe
15. Potentia
16. Sensitive Molecular Probes in Energy
17. Transducing Organelles
Loew, Leslie M.