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E-raamat: Lipid-Mediated Signaling [Taylor & Francis e-raamat]

Edited by (University of North Dakota, School of Medicine and Health Sciences, Grand Forks, USA), Edited by (University of North Dakota, Grand Forks, USA), Edited by (University of North Dakota, Grand Forks, USA), Edited by (University of North Dakota, Grand Forks, USA)
  • Formaat: 452 pages, 37 Tables, black and white; 58 Illustrations, black and white
  • Sari: Methods in Signal Transduction Series
  • Ilmumisaeg: 05-Mar-2010
  • Kirjastus: CRC Press Inc
  • ISBN-13: 9780429128448
  • Taylor & Francis e-raamat
  • Hind: 327,75 €*
  • * hind, mis tagab piiramatu üheaegsete kasutajate arvuga ligipääsu piiramatuks ajaks
  • Tavahind: 468,21 €
  • Säästad 30%
  • Formaat: 452 pages, 37 Tables, black and white; 58 Illustrations, black and white
  • Sari: Methods in Signal Transduction Series
  • Ilmumisaeg: 05-Mar-2010
  • Kirjastus: CRC Press Inc
  • ISBN-13: 9780429128448
As the highly anticipated update to Lipid Second Messengers (CRC Press, 1999), Lipid-Mediating Signaling is a current and comprehensive overview of research methods used in lipid-mediated signal transduction. Pioneering experts provide a much-needed distillation of a decades worth of advances in research techniques that are pertinent in understanding how lipid-mediated signal transduction ties to pathologic disorders.

Part I describes methods used to identify activities of and assay the expression of different enzymes involved in lipid-mediated signaling. Part II highlights the use of mass spectrometry to ascertain the lipid content in various systems. Part III contains two chapters devoted to techniques used to determine the role of lipids in the activation of gene transcription. Part IV rounds out the books solid coverage by focusing on methods used to assess fatty acid uptake and metabolism.

Lipid-Mediating Signaling is an unrivaled reference for this rapidly expanding, yet resource-limited field, and it is a valuable resource for for cell biologists, biochemists, and pharmacologists, as well as for researchers studying signaling transduction.
Series Preface xi
Preface xiii
Editors xv
Contributors xvii
Part I Enzyme Systems Involved in Lipid-Mediated Signaling
Methods for Measuring the Activity and Expression of Phospholipases A2
3(24)
Prerna Rastogi
Janhavi Sharma
Jane McHowat
Methods of Measuring the Activity and Expression of Phospholipases C
27(28)
Ryan A. Frieler
John A. Watt
Thad A. Rosenberger
Methods for Measuring the Activity and Expression of Phospholipase D
55(22)
Wenjuan Su
Michael A. Frohman
Methods for Measuring the Activity and Expression of Diacylglycerol Kinase
77(32)
Kaoru Goto
Yasukazu Hozumi
Tomoyuki Nakano
Alberto M. Martelli
Hisatake Kondo
Methods for Measuring Endocannabinoid Production and Expression and Activity of Enzymes Involved in the Endocannabinoid System
109(42)
Tiziana Bisogno
Luciano De Petrocellis
Vincenzo Di Marzo
Part II Mass Spectrometry Methods for Lipid Analysis
Mass Spectrometry Methods for the Analysis of Lipid Molecular Species: A Shotgun Lipidomics Approach
151(26)
Xianlin Han
Hua Cheng
Xuntian Jiang
Youchun Zeng
Mass Spectrometry Methods for the Analysis of Bioactive Sphingolipids: A High-Performance Liquid Chromatography/Tandem Mass Spectrometry Approach
177(22)
Jacek Bielawski
Motohiro Tani
Yusuf A. Hannun
Mass Spectrometry Methods for the Analysis of Oxidant Stress in Biological Fluids and Tissues: Quantification of F2-Isoprostanes and F4-Neuroprostanes Using Mass Spectrometry
199(16)
Ginger L. Milne
Jason D. Morrow
Part III Lipid-Mediated Regulation of Gene Expression
Methods for Assessing the Role of Lipid-Activated Nuclear Receptors in the Regulation of Gene Transcription
215(84)
Jenny Kaeding
Jocelyn Trottier
Olivier Barbier
Fluorescence Methods to Assess the Impact of Lipid Binding Proteins on Ligand-Mediated Activation of Gene Expression
299(52)
Heather A. Hostetler
Avery L. McIntosh
Anca D. Petrescu
Huan Huang
Barbara P. Atshaves
Eric J. Murphy
Ann B. Kier
Friedhelm Schroeder
Part IV Methods to Assess Fatty Acid and Phospholipid Metabolism
Methods for Measuring Fatty Acids Using Chromatographic Techniques
351(22)
Gwendolyn Barcelo-Coblijn
Cameron C. Murphy
Eric J. Murphy
Methods for Measuring Fatty Acid Uptake and Targeting in Cultured Cells and in Vivo
373(22)
Paul G. Millner
Serena M. Lackman
Eric J. Murphy
Methods for Kinetic Analysis of Fatty Acid Incorporation and Turnover in Vivo: A Steady-State Kinetic Radiotracer Approach
395(20)
Dhaval P. Bhatt
Thad A. Rosenberger
Index 415
Eric J. Murphy, University of North Dakota, Grand Forks, USA Thad A. Rosenberger, University of North Dakota, Grand Forks, USA