Editors Doglia and Lotti offer this compilation on bacterial protein aggregation via inclusion bodies. A detailed introduction to protein folding processes and dynamics opens the book, followed by chapters on recruitment of unfolding chaperones for use on misfolded recombinant proteins and use of osmolytes for chaperone activity. The relevance of inclusion bodies to amyloid aggregation and eukaryotic aggregation is discussed, as well as their structural properties and potential biomedical applications. Finally, recombinant protein aggregation as a production and design issue is addressed. Annotation ©2014 Ringgold, Inc., Portland, OR (protoview.com)
Focuses on the aggregation of recombinant proteins in bacterial cells in the form of inclusion bodies—and on their use in biotechnological and medical applications
The first book devoted specifically to the topic of aggregation in bacteria, Protein Aggregation in Bacteria: Functional and Structural Properties of Inclusion Bodies in Bacterial Cells provides a large overview of protein folding and aggregation, including cell biology and methodological aspects. It summarizes, for the first time in one book, ideas and technical approaches that pave the way for a direct use of inclusion bodies in biotechnological and medical applications.
Protein Aggregation in Bacteria covers:
- Molecular and cellular mechanisms of protein folding, aggregation, and disaggregation in bacteria
- Physiological importance and consequences of aggregation for the bacterial cell
- Factors inherent to the protein sequence responsible for aggregation and evolutionary mechanisms to keep proteins soluble
- Structural properties of proteins expressed as soluble aggregates and as inclusion bodies within bacterial cells both from a methodological point of view and with regard to their similarity with amyloids
- Control of the structural and functional properties of aggregated proteins and use thereof in biotechnology and medicine
Protein Aggregation in Bacteria is ideal for researchers in protein science, biochemistry, bioengineering, biophysics, microbiology, medicine, and biotechnology, particularly if they are related with the production of recombinant proteins and pharmaceutical science.
Rohkem infot Wiley Online kohta