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Adipogenesis: Methods and Protocols [Kõva köide]

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  • Formaat: Hardback, 189 pages, kõrgus x laius: 254x178 mm, 40 Illustrations, color; 7 Illustrations, black and white; X, 189 p. 47 illus., 40 illus. in color., 1 Hardback
  • Sari: Methods in Molecular Biology 2938
  • Ilmumisaeg: 31-May-2025
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071646060
  • ISBN-13: 9781071646069
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  • Formaat: Hardback, 189 pages, kõrgus x laius: 254x178 mm, 40 Illustrations, color; 7 Illustrations, black and white; X, 189 p. 47 illus., 40 illus. in color., 1 Hardback
  • Sari: Methods in Molecular Biology 2938
  • Ilmumisaeg: 31-May-2025
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071646060
  • ISBN-13: 9781071646069
Teised raamatud teemal:

This volume details protocols in Adipogensis research, such as stem cell line isolation, gene expression, obesity research, and drug development. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Adipogenesis: Methods and Protocols aims to be comprehensive guide for researchers in the field.

Adipogenic differentiation from human embryonic stem cells through
formation of embryoid bodies.- Quantification of adipogenesis using flow
cytometry.- Isolation of Whartons Jelly derived Mesenchymal Stromal/Stem
Cells.- Adipogenic differentiation assay for the study of insulin
signalling.- Quantification of lipid droplets in adipocytes.- RT-qPCR
quantification of adipogenic marker genes (Pparg, C/ebpa, Fabp4 and Cd36).-
Adipogenic differentiation of human adipose-derived mesenchymal stromal/stem
cells (ASCs).- Western blot detection of adipogenic protein.- Measurement of
intracellular Reactive Oxygen Species during adipogenesis.- Oxygen
consumption and extra cellular acidification rate in adipogenic
differentiated human mesenchymal stromal/stem cells.- In vitro expansion of
human adipose-derived stem cells (ASCs).- siRNA silencing of adipogenic gene
in ASCs.- Freezing and thawing of Adipose-derived stromal/stem cells (ASCs).-
Isolation and Characterization of Mouse Adipose-Derived Stromal/Stem Cells.-
Isolation of adipose-derived mesenchymal stromal/stem cells.- Isolation and
purification of adipocyte-derived extracellular vesicles (AdEVs) using
size-exclusion chromatography.- Differentiation of Adipose derived
stromal/stem cells into Osteocytes.- Differentiation of mesenchymal
stromal/stem cells from induced pluripotent stem cells.- Isolation and
Characterization of Equine Mezenchymal Stromal/Stem Cell.