This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a "fingerprint" for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in dia
monds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.
Modern TCSPC electronics - Principles and Acquisition ModesMichael WahlSingle-photon counting detectors for the visible range between 300 nm and 1000 nmAndreas BülterSingle-photon detectors for infrared wavelengths in the range 1 to 1.7 mGerald S. Buller and Robert J. CollinsModern pulsed diode laser sources for time-correlated photon countingKristian Lauritsen, Sina Riecke, Andreas Bülter, Thomas SchönauAdvanced FCS: an Introduction to Fluorescence Lifetime Correlation Spectroscopy and Dual Focus FCSThomas Dertinger and Steffen RüttingerLifetime-weighted FCS and 2D FLCS: Advanced application of time-tagged TCSPCKunihiko Ishii, Takuhiro Otosu, and Tahei TaharaMFD-PIE and PIE-FI: Ways to extract more information with TCSPCAnders Barth, Lena Voith von Voithenberg, Don C. LambPhoton Antibunching in Single Molecule Fluorescence SpectroscopyKristin S. Grußmayer and Dirk-Peter HertenFLIM Strategies for Intracellular Sensing: Fluorescence Lifetime Imaging
as a Tool to Quantify Analytes of InterestMaria J. Ruedas-Rama, Jose M. Alvarez-Pez, Luis Crovetto, Jose M. Paredes, Angel OrteMultiple-Pulse Pumping with Time-Gated Detection for Enhanced Fluorescence Imaging in Cells and TissueRafal Fudala, Ryan M. Rich, Joe Kimball, Ignacy Gryczynski, Sangram Raut, Julian Borejdo, Dorota L. Stankowska, Raghu R. Krishnamoorthy, Karol Gryczynski, Badri P. Maliwal, Zygmunt GryczynskiPattern based linear un-mixing for efficient and reliable analysis of multi-component TCSPC-dataIngo Gregor and Matthias PattingMetal-Induced Energy TransferNarain Karedla, Daja Ruhlandt, Anna M. Chizhik, Jörg Enderlein, Alexey I. ChizhikThe importance of photon arrival times in STED microscopyGiuseppe VicidominiSingle color centers in diamond as single photon sources and quantum sensorsBoris Naydenov and Fedor JelezkoPhoton counting and timing in quantum optics experimentsAndreas Ahlrichs, Benjamin Sprenger, Oliver BensonPhoton counting in d
iffuse optical imagingDirk Grosenick
