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Bacterial Efflux Systems, Volume 724 [Kõva köide]

Volume editor (Department of Biochemistry, University of Cambridge, U.K.)
  • Formaat: Hardback, 564 pages, kõrgus x laius: 229x152 mm, kaal: 450 g
  • Sari: Methods in Enzymology
  • Ilmumisaeg: 24-Nov-2025
  • Kirjastus: Academic Press Inc
  • ISBN-10: 0443429596
  • ISBN-13: 9780443429590
Teised raamatud teemal:
Bacterial Efflux Systems, Volume 724Suurem pilt
  • Formaat: Hardback, 564 pages, kõrgus x laius: 229x152 mm, kaal: 450 g
  • Sari: Methods in Enzymology
  • Ilmumisaeg: 24-Nov-2025
  • Kirjastus: Academic Press Inc
  • ISBN-10: 0443429596
  • ISBN-13: 9780443429590
Teised raamatud teemal:
Püsilink: https://www.kriso.ee/db/9780443429590.html
Märksõnad:
Bacterial Efflux Systems, Volume 722 covers experimental and computational approaches to study the function, structure, and mechanism of bacterial efflux pump systems. The methods covered include preparation of samples for structural and functional analyses and in vivo activity analysis. Specific chapters in this release cover Efflux pump functional assays, NMR and functional analysis efflux pumps, Methods for saturation mutagenesis and functional characterization, Cooperation and functional interplay between pumps, Mapping interactions, HDX-MS, and much more.

Other sections cover Recombinant Expression and purification, Reconstitution of a RND efflux pump in different artificial membranes for a structural biology approach, Reconstitution of rnd and mfs efflux pumps for structural biology, CusCBA system, Expression and purification of efflux pumps of Gram positives, Expression and purification of efflux pumps of archaea, ABC transporters: BmrA preparation for NMR and PatA/PatB in peptidisc, In situ structural analysis of efflux assemblies by cryoET, Molecular dynamics simulations, and so much more.
1. Efflux pump functional assays
2. NMR and functional analysis efflux pumps
3. Methods for saturation mutagenesis and functional characterisation
4. Cooperation and functional interplay between pumps
5. Mapping interactions, HDX-MS

Section II: Recombinant Expression and purification
6. Reconstitution of a RND efflux pump in different artificial membranes for
a structural biology approach
7. Reconstitution of rnd and mfs efflux pumps for structural biology
8. CusCBA system
9. Expression and purification of efflux pumps of Gram positives
10. Expression and purification of efflux pumps of archaea
11. ABC transporters: BmrA preparation for NMR and PatA/PatB in peptidisc

Section III. Structural and computational studies
12. In situ structural analysis of efflux assemblies by cryoET
13. Molecular dynamics simulations
14. CryoEM analysis of efflux pumps
15. Crystallographic studies of transporters with transport substrates and
inhibitors

Section IV. In vivo expression and activity and physiological roles
16. Assembly and biogenesis
17. Heterogeneity of efflux pump expression in populations
Ben Luisi works in the Department of Biochemistry at the University of Cambridge, where he has an appointment as professor of structural biology. He and his colleagues study the structure and mechanism of efflux pumps and the riboregulatory machinery that help to control the expression of the pumps and other complex systems in bacteria.