Biological Macromolecule Engineering Technologies [Kõva köide]

High Level Protein Expression in Pseudomonas putida Using the
Phage-Based pPUT Expression System.- New-to-Nature Photoenzymatic Catalysis.-
CRISPR-Hybrid: Intracellular Selection of CRISPR-Associated Aptamers in
Bacteria.- Designing and Engineering Enzymes for New-to-Nature
Photobiocatalysis.- A Yeast Two-Hybrid Assay to Develop Optogenetic Tools for
Controlling Protein-Protein Interactions.- CRISPR-Engineered Bacteriophage T4
for Foot-and-Mouth Disease Nanoparticle Vaccine Development.-
Endoskeleton-Enhanced Virus-Like Particles (VLPs): Internal Cross-Linking of
Physalis Mottle Virus and Stability Tests.- An Integrated Workflow for Phage
Selection, Amplification, and Surface Modification.- CRISPR-Cpf1-Mediated T4
Phage Genome Editing for One-Step In Vivo Display of Heterologous Protein.-
Methods for Applying Prime Editing and Inverse Prime Editing in Mammalian
Cell Culture.- Constructing Drive-and-Process (DAP) CRISPR Guide RNA Arrays
for Multiplexed Base- and Prime-Editing.- Production of Cell Models with
Differential Zygosity Using CRISPR Base Editors.- Phage-Assisted Continuous
Selection of Bioactive Cyclic Peptides.- Targeted Transition Mutations in
Phage-Assisted Evolution.- Phage Display In Vivo: Protocols for Identifying
Brain-Targeting Peptides in Mouse Models of Neurodegeneration.- Generation of
Diversely Functionalized DNA Libraries Using Ligase-Catalyzed Oligonucleotide
Polymerization (LOOPER).- Solid Phase Chemical Synthesis of RNA.-
Fluorescence-Based Assays to Evaluate the Functionality of Phage-Derived
Regulatory Elements for Applications in Synthetic Biology.- Using peCHYRON to
Record Mammalian Cells History of Chemical Exposure into Their DNA.