Dedication
Preface
Acknowledgement
Chapter 1 De novo Peptide Design Tools
Chapter 2 Molecular Docking
Chapter 3 Molecular Dynamics Simulations
Chapter 4 Peptide Synthesis
Chapter 5 Mass Spectrometry
Chapter 6 Circular Dichroism (CD) Spectroscopy
Chapter 7 Fourier Transform Infrared (FTIR) Spectroscopy
Chapter 8 Cell Culture for Cell Penetrating Peptides
Chapter 9 Comparative Uptake of Peptides by Flow Cytometry
Chapter 10 Cellular uptake of peptides by confocal microscopy
Chapter 11 Penetration in 3D Tumorospheres
Chapter 12 TMRM Cytotoxicity Assay
Chapter 13 Cell Death by Apoptosis
Chapter 14 Hemolytic activity
Chapter 15 Antimicrobial Assay
Chapter 16 Antimicrobial assay with Peptide Based Hydrogels
Chapter 17 Rheology of Peptide based Hydrogels
Chapter 18 Estimation of Aggregation Kinetics
Chapter 19 Assessment of Amyloid morphology using Electron Microscopy
Chapter 20 Catalytic Efficiency of the Designed Peptides
Dr. Vibin Ramakrishnan is a Professor of Biosciences and Bioengineering at IIT Guwahati. He did his Masters in Applied Chemistry from Cochin University of Science and Technology and Ph.D. from School of Biosciences and Bioengineering, IIT Bombay. He worked as a Postdoctoral Research Associate at Rennsselaer Polytechnic Institute, New York and a Faculty scientist at IBAB Bangalore, before joining Department of Biosciences & Bioengineering at IITG. He has published more than 50 research articles in reputed international journals and filed 10 patents, including an international patent to his credit. He is also the principal scientific advisor of two biotech companies.
The Department of Biotechnology, Govt. of India has awarded Dr. Vibin, the Innovative Young Biotechnologist Award (IYBA) in the year 2007-08 to recognize his contributions and for innovative approach in biotechnology research. Dr. Vibin is an editorial board member of Scientific Reports. He has more than15 years of teaching experience in Biophysics, Computational Biology, Bio-techniques, Analytical Biotechnology, and Proteomics. He is a member of many international scientific societies such as American Chemical Society, Biophysical Society, and Protein Society.