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Bioseparations Science and Engineering 3rd Revised edition [Kõva köide]

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(David Ross Boyd Professor, University of Oklahoma), (Founder and Principal Consultant, Syner-G Biopharma Group), (President and CEO, Intelligen, Inc.), (Chief Scientist Emeritus, RedWire Space, Inc.)
  • Formaat: Hardback, 536 pages, kõrgus x laius: 254x178 mm, 197 b/w figures
  • Sari: Topics in Chemical Engineering
  • Ilmumisaeg: 04-Feb-2026
  • Kirjastus: Oxford University Press Inc
  • ISBN-10: 0197672507
  • ISBN-13: 9780197672501
Teised raamatud teemal:
Bioseparations Science and Engineering 3rd Revised editionSuurem pilt
  • Formaat: Hardback, 536 pages, kõrgus x laius: 254x178 mm, 197 b/w figures
  • Sari: Topics in Chemical Engineering
  • Ilmumisaeg: 04-Feb-2026
  • Kirjastus: Oxford University Press Inc
  • ISBN-10: 0197672507
  • ISBN-13: 9780197672501
Teised raamatud teemal:
Püsilink: https://www.kriso.ee/db/9780197672501.html
Märksõnad:
"Current, comprehensive, and concise, Bioseparations Science and Engineering covers bioseparation unit operations in depth. The field of bioseparations has developed as a significant, separate dis-cipline within the general field of biochemical engineering that involves the separation and purifi-cation of compounds of biological origin. The biotechnology industry has given added im-portance to bioseparations, because many of the products of biotechnology are proteins that often are difficult to purify and frequently must be purified to homogeneity or near homogeneity, lead-ing to high costs. Designed for undergraduates, graduate students, and industry practitioners, Bioseparations Science and Engineering fills a critical need in the field. The unit operations covered in the book are cell lysis, flocculation, filtration, sedimentation, extraction, liquid chromatography, liquid adsorption, precipitation, crystallization, evaporation, and drying. In each of the chapters, the authors use a consistent method of explaining unit operations, starting with a qualitative de-scription noting the significance and general application of the unit operation. They then illustrate the scientific application of the operation, develop the required mathematical theory, and finally, describe the applications of the theory in engineering practice, with an emphasis on design and scale-up. Unique to this text is a chapter dedicated to bioseparations process design and econom-ics, in which a process simulator, SuperPro Designer(r), is used to analyze and evaluate the pro-duction of six important biological products. Additonal unique features are a chapter about ana-lytical methods and bench scale bioseparations and a chapter with laboratory exercises in biosepa-rations"-- Provided by publisher.

Designed for undergraduates, graduate students, and industry practitioners, the third edition of Bioseparations Science and Engineering fills a critical need in the field. Current, comprehensive, and concise, it covers bioseparations unit operations in unprecedented depth. The unit operations covered are cell lysis, flocculation, filtration, sedimentation, extraction, liquid chromatography, liquid adsorption, precipitation, crystallization, evaporation, and drying.

Designed for undergraduates, graduate students, and industry practitioners, the third edition of Bioseparations Science and Engineering fills a critical need in the field. Current, comprehensive, and concise, it covers bioseparations unit operations in unprecedented depth. The unit operations covered are cell lysis, flocculation, filtration, sedimentation, extraction, liquid chromatography, liquid adsorption, precipitation, crystallization, evaporation, and drying.

In each of the chapters, the authors use a consistent method of explaining unit operations, starting with a qualitative description noting the significance and general application of the unit operation. They then illustrate the scientific application of the operation, develop the required mathematical theory, and finally, describe the applications of the theory in engineering practice, with an emphasis on design and scale-up. Unique to this text is a chapter dedicated to bioseparations process design and economics, in which a process simulator, SuperPro Designer®, is used to analyze and evaluate the production of six important biological products.

The third edition of the book has been completely updated and contains the addition of several topics, including the stability of bioproducts, electrophoretic analysis of DNA and RNA, separation by flow cytometry, continuous crystallization, batch crystallization by cooling, fluidized bed drying, and process design and economics of the production of messenger RNA vaccine, hyaluronic acid, and monosodium glutamate. Unique features include basic information about bioproducts, descriptions of analytical methods and bench scale separations of bioproducts, and a chapter with bioseparations laboratory exercises. Bioseparations Science and Engineering is ideal for students and professionals working in or studying bioseparations and is the premier text in the field.
Preface xix
1. Introduction to Bioproducts and Bioseparations 1.1
Instructional Objectives 1.2 Broad Classification of Bioproducts 1.3 Small
Biomolecules 1.3.1 Primary Metabolites 1.3.2 Secondary Metabolites 1.3.3
Stability of Small Biomolecules
1.3.4 Summary of Small Biomolecules 1.4 Macromolecules: Proteins 1.4.1
Primary Structure 1.4.2 Secondary Structure 1.4.3 Tertiary Structure Example
1.1. Effect of a Reducing Agent on Protein Structure and Mobility 1.4.4
Quaternary Structure 1.4.5 Prosthetic Groups and Hybrid Molecules 1.4.6
Functions and Commercial Uses of Proteins 1.4.7 Stability of Proteins 1.4.8
Recombinant Protein Expression 1.5 Macromolecules: Nucleic Acids and
Oligonucleotides 1.5.1 Structure of Nucleic Acids 1.5.2 Functions and
Commercial Uses 1.5.3 Stability of Nucleic Acids 1.6 Macromolecules:
Polysaccharides 1.7 Particulate Products 1.8 Introduction to Bioseparations:
Engineering Analysis 1.8.1 Stages of Downstream Processing Example 1.2.
Initial Selection of Purification Steps 1.8.2 Basic Principles of Engineering
Analysis 1.8.3 Process and Product Quality 1.8.4 Criteria for Process
Development 1.9 The Route to Market 1.9.1 The Chemical and Applications Range
of the Bioproduct 1.9.2 Documentation of Pharmaceutical Bioproducts 1.9.3 GLP
and cGMP 1.9.4 Formulation 1.10 Summary Nomenclature Problems References
2.
Analytical Methods and Bench Scale Preparative Bioseparations 2.1
Instructional Objectives 2.2 Specifications 2.3 Assay Attributes 2.3.1
Precision 2.3.2 Accuracy 2.3.3 Specificity 2.3.4 Linearity, Limit of
Detection, and Limit of Quantitation 2.3.5 Range 2.3.6 Robustness 2.4
Analysis of Biological Activity 2.4.1 Animal Model Assays 2.4.2
Cell-Line-Derived Bioassays 2.4.3 In vitro Biochemical Assays Example 2.1.
Coupled Enzyme Assay for Alcohol Oxidase 2.5 Analysis of Purity 2.5.1
Electrophoretic Analysis Example 2.2. Estimation of the Maximum Temperature
in an Electrophoresis Gel 2.5.2 High-Performance Liquid Chromatography (HPLC)
2.5.3 Mass Spectrometry 2.5.4 Coupling of HPLC with Mass Spectrometry 2.5.5
Ultraviolet Absorbance Example 2.3. Determination of Molar Absorptivity 2.5.6
CHNO/Amino Acid Analysis (AAA) Example 2.4. Calculations Based on CHNO
Analysis 2.5.7 Protein Assays 2.5.8 Enzyme-Linked Immunosorbent Assay 2.5.9
Gas Chromatography 2.5.10 DNA Hybridization 2.5.11 ICP/MS (AES) 2.5.12 Dry
Weight 2.5.13 Flow Cytometry
2.6 Microbiology Assays 2.6.1 Sterility 2.6.2 Bioburden 2.6.3 Endotoxin 2.6.4
Virus, Mycoplasma, and Phage 2.7 Bench Scale Preparative Separations 2.7.1
Preparative Electrophoresis 2.7.2 Magnetic Bioseparations
2.7.3 Cell Purification by Flow Cytometry 2.8 Summary Nomenclature Problems
References
3. Cell Lysis and Flocculation 3.1 Instructional Objectives 3.2
Some Elements of Cell Structure 3.2.1 Prokaryotic Cells 3.2.2 Eukaryotic
Cells 3.3 Cell Lysis 3.3.1 Osmotic and Chemical Cell Lysis 3.3.2 Mechanical
Methods of Lysis 3.4 Flocculation 3.4.1 The Electric Double Layer Example
3.1. Dependence of the Debye Radius on the Type of Electrolyte 3.4.2 Forces
Between Particles and Flocculation by Electrolytes Example 3.2. Sensitivity
of Critical Flocculation Concentration to Temperature and Counterion Charge
Number 3.4.3 The Schulze-Hardy Rule 3.4.4 Flocculation Rate 3.4.5 Polymeric
Flocculants 3.5 Summary Nomenclature Problems References
4. Filtration 4.1
Instructional Objectives 4.2 Filtration Principles 4.2.1 Conventional
Filtration Example 4.1. Batch Filtration 4.2.2 Crossflow Filtration Example
4.2. Concentration Polarization in Ultrafiltration Example 4.3. Comparison of
Mass Transfer Coefficient Calculated by Boundary Layer Theory Versus by
Shear-Induced Diffusion Theory 4.3 Filter Media and Equipment 4.3.1
Conventional Filtration 4.3.2 Crossflow Filtration 4.4 Membrane Fouling 4.5
Scale-up and Design of Filtration Systems 4.5.1 Conventional Filtration
Example 4.4. Rotary Vacuum Filtration Example 4.5. Washing of a Rotary Vacuum
Filter Cake 4.5.2 Crossflow Filtration Example 4.6. Diafiltration Mode in
Crossflow Filtration 4.6 Summary Nomenclature Problems References
5.
Sedimentation 5.1 Instructional Objectives 5.2 Sedimentation Principles 5.2.1
Equation of Motion 5.2.2 Sensitivities 5.3 Methods for Analysis of
Sedimentation 5.3.1 Equilibrium Sedimentation 5.3.2 Sedimentation Coefficient
Example 5.1. Application of the Sedimentation Coefficient 5.3.3 Equivalent
Time Example 5.2. Scale-up Based on Equivalent Time 5.3.4 Sigma Analysis 5.4
Production Centrifuges: Comparison and Engineering Analysis 5.4.1 Tubular
Bowl Centrifuge Example 5.3. Complete Recovery of Bacterial Cells in a
Tubular Bowl Centrifuge 5.4.2 Disk Centrifuge 5.5 Ultracentrifugation 5.5.1
Determination of Molecular Weight 5.6 Flocculation and Sedimentation 5.7
Sedimentation at Low Accelerations 5.7.1 Diffusion, Brownian Motion 5.7.2
Isothermal Settling 5.7.3 Convective Motion and Péclet Analysis 5.7.4
Inclined Sedimentation 5.7.5 Field-Flow Fractionation 5.8 Centrifugal
Elutriation 5.9 Summary Nomenclature Problems References
6. Extraction 6.1
Instructional Objectives 6.2 Extraction Principles 6.2.1 Phase Separation and
Partitioning Equilibria Example 6.1 Process for Large-Scale Isolation of
?-Galactosidae from E. coli in an Aqueous Two-Phase Sytstem
6.2.2 Countercurrent Stage Calculations Example 6.2. Separation of a
Bioproduct and an Impurity by Countercurrent Extraction Example 6.3. Effect
of Solvent Rate in Countercurrent Staged Extraction of an Antibiotic 6.3
Scale-up and Design of Extractors 6.3.1 Reciprocating-Plate Extraction
Columns Example 6.4. Scale-up of a Reciprocating-Plate Extraction Column
6.3.2 Centrifugal Extractors Example 6.5. Increase in Feed Rate to a
Podbielniak Centrifugal Extractor 6.4 Summary Nomenclature Problems
References
7. Liquid Chromatography and Adsorption 7.1 Instructional
Objectives 7.2 Adsorption Equilibrium 7.3 Adsorption Column Dynamics 7.3.1
Fixed-Bed Adsorption Example 7.1. Determination of the Mass Transfer
Coefficient from Adsorption Breakthrough Data 7.3.2 Agitated-Bed Adsorption
7.4 Chromatography Column Dynamics 7.4.1 Plate Models 7.4.2 Moment Analysis
Example 7.2 Calculation of the HETP Using the Method of Moments
7.4.3 Chromatography Column Mass Balance with Negligible Dispersion Example
7.3. Chromatographic Separation of Two Solutes Example 7.4. Calculation of
the Shock Wave Velocity for a Nonlinear Isotherm Example 7.5. Calculation of
the Elution Profile 7.4.4 Dispersion Effects in Chromatography 7.4.5 Computer
Simulation of Chromatography Considering Axial Dispersion, Fluid-Phase Mass
Transfer, Intraparticle Diffusion, and Nonlinear Equilibrium Example 7.6
Computer Simulation of a Chromatography Process 7.4.6 Gradients and Modifiers
Example 7.7. Equilibrium for a Protein Anion in the Presence of Chloride Ion
7.5 Membrane Chromatography Example 7.8. Comparison of Time for Diffusion
Mass Transfer in Conventional Chromatography and Membrane Chromatography 7.6
Simulated Moving Bed Chromatography 7.7 Adsorbent Types 7.7.1 Silica-Based
Resins 7.7.2 Polymer-Based Resins 7.7.3 Ion Exchange Chromatography and
Adsorption 7.7.4 Reversed-Phase Chromatography 7.7.5 Hydrophobic Interaction
Chromatography 7.7.6 Affinity Chromatography 7.7.7 Immobilized Metal Affinity
Chromatography (IMAC) 7.7.8 Size Exclusion Chromatography 7.8 Particle Size
and Pressure Drop in Fixed Beds 7.9 Equipment 7.9.1 Columns 7.9.2
Chromatography Column Packing Procedures 7.9.3 Detectors 7.9.4 Chromatography
System Fluidics 7.10 Scale-up 7.10.1 Adsorption Example 7.9. Scale-up of the
Fixed-Bed Adsorption of a Pharmaceutical Product 7.10.2 Chromatography
Example 7.10. Scale-up of a Protein Chromatography Example 7.11. Scale-up of
Protein Chromatography Using Standard Column Sizes Example 7.12. Scale-up of
Elution Buffer Volumes in Protein Chromatography Example 7.13. Consideration
of Pressure Drop in Column Scaling 7.11 Summary Nomenclature Problems
References
8. Precipitation 8.1 Instructional Objectives 8.2 Protein
Solubility 8.2.1 Structure and Size 8.2.2 Charge 8.2.3 Solvent Example 8.1.
Salting Out of a Protein with Ammonium Sulfate 8.3 Precipitate Formation
Phenomena 8.3.1 Initial Mixing 8.3.2 Nucleation 8.3.3 Growth Governed by
Diffusion Example 8.2. Calculation of Concentration of Nuclei in a Protein
Precipitation Example 8.3. Diffusion-Limited Growth of Particles 8.3.4 Growth
Governed by Fluid Motion Example 8.4. Growth of Particles Limited by Fluid
Motion 8.3.5 Precipitate Breakage 8.3.6 Precipitate Aging 8.4 Particle Size
Distribution in a Continuous-Flow Stirred Tank Reactor Example 8.5.
Dependence of Population Density on Particle Size and Residence Time in a
CSTR 8.5 Methods of Precipitation 8.6 Design of Precipitation Systems 8.7
Summary Nomenclature Problems References
9. Crystallization 9.1 Instructional
Objectives 9.2 Crystallization Principles 9.2.1 Crystals 9.2.2 Nucleation
9.2.3 Crystal Growth 9.2.4 Crystallization Kinetics from Batch Experiments
9.3 Batch Crystallizers 9.3.1 Analysis of Dilution Batch Crystallization
Example 9.1. Batch Crystallization with Constant Rate of Change of Diluent
Concentration 9.3.2 Cooling Batch Crystallization Example 9.2 Batch
Crystallization by Cooling 9.4 Continuous Crystallization
Example 9.3 Calculation of the Population Density and the Growth and
Nucleation Rates for a MSMPR Crystallizer
9.5 Process Crystallization of Proteins 9.6 Crystallizer Scale-up and Design
9.6.1 Experimental Crystallization Studies as a Basis for Scale-up 9.6.2
Scale-up and Design Calculations Example 9.4. Scale-up of Crystallization
Based on Constant Power per Volume 9.7 Summary Nomenclature Problems
References
10. Evaporation 10.1 Instructional Objectives 10.2 Evaporation
Principles 10.2.1 Heat Transfer Example 10.1. Evaporation of a Butyl Acetate
Stream Containing a Heat-Sensitive Antibiotic in a Falling-Film Evaporator
10.2.2 Vapor-Liquid Separation 10.3 Evaporation Equipment 10.3.1
Climbing-Film Evaporators 10.3.2 Falling-Film Evaporators 10.3.3
Forced-Circulation Evaporators 10.3.4 Agitated-Film Evaporators 10.4 Scale-up
and Design of Evaporators 10.5 Summary Nomenclature Problems References
11.
Drying 11.1 Instructional Objectives 11.2 Drying Principles 11.2.1 Water in
Biological Solids and in Gases Example 11.1. Drying of Antibiotic Crystals
11.2.2 Heat and Mass Transfer Example 11.2. Conductive Drying of Wet Solids
in a Tray Example 11.3. Mass Flux During the Constant Rate Drying Period in
Convective Drying Example 11.4. Time to Dry Nonporous Biological Solids by
Convective Drying 11.3 Dryer Description and Operation 11.3.1 Vacuum-Shelf
Dryers 11.3.2 Batch Vacuum Rotary Dryers 11.3.3 Freeze Dryers 11.3.4 Spray
Dryers 11.5 Fluidized Bed Dryers
11.4 Scale-up and Design of Drying Systems 11.4.1 Vacuum-Shelf Dryers 11.4.2
Batch Vacuum Rotary Dryers 11.4.3 Freeze Dryers 11.4.4 Spray Dryers Example
11.5. Sizing of a Spray Dryer 11.4.5 Fluidized Bed Dryers Example 11.6
Scale-up of a Fluidized Bed Dryer
11.5 Summary Nomenclature Problems References
12. Bioprocess Design and
Economics 12.1 Instructional Objectives 12.2 Definitions and Background 12.3
Synthesis of Bioseparation Processes 12.3.1 Primary Recovery Stages 12.3.2
Intermediate Recovery Stages 12.3.3 Final Purification Stages 12.3.4 Pairing
of Unit Operations in Process Synthesis 12.4 Process Analysis 12.4.1
Spreadsheets 12.4.2 Process Simulators and Their Benefits 12.4.3 Using a
Biochemical Process Simulator 12.5 Process Economics 12.5.1 Capital Cost
Estimation 12.5.2 Operating Cost Estimation 12.5.3 Profitability Analysis
12.6 Illustrative Examples 12.6.1 Citric Acid Production 12.6.2 Human Insulin
Production 12.6.3 Therapeutic Monoclonal Antibody Production 12.6.4 RNA
(mRNA) Vaccine Production
12.6.5 Hyaluronic Acid Production
12.6.6 Monosodium Glutamate (MSG) Production
12.7 Summary Problems References
13. Laboratory Exercises in Bioseparations
13.1 Flocculant Screening 13.1.1 Background 13.1.2 Objectives 13.1.3
Procedure 13.1.4 Report 13.1.5 Some Notes and Precautions 13.2 Crossflow
Filtration 13.2.1 Background 13.2.2 Objectives 13.2.3 Procedure 13.2.4 Report
13.3 Centrifugation of Flocculated and Unflocculated Particulates 13.3.1
Background 13.3.2 Objectives 13.3.3 Procedure 13.3.4 Report 13.4 Aqueous
Two-Phase Extraction 13.4.1 Physical Measurements 13.4.2 Procedure 13.4.3
Calculations and Report 13.4.4 Inverse Lever Rule 13.5 Chromatography
Scale-up 13.5.1 Background 13.5.2 Objectives 13.5.3 Procedure 13.5.4 Report
References APPENDIX: Table of Units and Constants Index
Dr. Roger G. Harrison is the David Ross Boyd Professor of Sustainable Chemical, Biological and Materials Engineering at the University of Oklahoma.



Dr. Paul W. Todd is retired Research Professor of Chemical Engineering, University of Colorado; and Chief Scientist Emeritus, Techshot, Inc. (now Redwire, Inc.).

Dr. Scott R. Rudge is a Founder and Principal Consultant with Syner-G Biopharma Group.

Dr. Demetri P. Petrides is the president of Intelligen, Inc., a software company that develops and markets simulation, design, and scheduling tools for the process manufacturing industries.