Calcium Entry Channels in Non-Excitable Cells focuses on methods of investigating the structure and function of non-voltage gated calcium channels. Each chapter presents important discoveries in calcium entry pathways, specifically dealing with the molecular identification of store-operated calcium channels which were reviewed by earlier volumes in the Methods in Signal Transduction series. Crystallographic and pharmacological approaches to the study of calcium channels of epithelial cells are also discussed. Calcium ion is a messenger in most cell types. Whereas voltage gated calcium channels have been studied extensively, the non-voltage gated calcium entry channel genes have only been identified relatively recently. The book will fill this important niche.
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vii | |
| Preface |
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| Contributors |
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Chapter 1 Electrophysiological Methods for Recording CRAC and TRPV5/6 Channels |
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1 | (24) |
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Chapter 2 Studies of Structure-Function and Subunit Composition of Orai/STIM Channel |
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25 | (26) |
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Chapter 3 Signaling ER Store Depletion to Plasma Membrane Orai Channels |
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51 | (22) |
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Chapter 4 Modulation of Orail and STIM1 by Cellular Factors |
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73 | (20) |
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Chapter 5 CRAC Channels and Ca2+-Dependent Gene Expression |
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93 | (14) |
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Chapter 6 Function of Orai/Stim Proteins Studied in Transgenic Animal Models |
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107 | (20) |
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Chapter 7 Assessing the Molecular Nature of the STIM1/Orai1 Coupling Interface Using FRET Approaches |
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127 | (18) |
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Chapter 8 Cytogenetic Approaches to Control Calcium Entry in Non-Excitable Cells |
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145 | (16) |
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Chapter 9 Regulation of Orai/STIM Channels by pH |
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161 | (16) |
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Chapter 10 Non-Orai Partners of STIM Proteins: Role in ER-PM Communication and Ca2+ Signaling |
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177 | (20) |
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Chapter 11 Store-Independent Orai Channels Regulated by STIM |
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197 | (18) |
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Chapter 12 Regulation and Role of Store-Operated Ca2+ Entry in Cellular Proliferation |
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215 | (26) |
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Chapter 13 TRPV5 and TRPV6 Calcium-Selective Channels |
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241 | (34) |
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Chapter 14 Determining the Crystal Structure of TRPV6 |
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275 | (18) |
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Chapter 15 Identifying TRP Channel Subunit Stoichiometry Using Combined Single Channel Single Molecule Determinations (SC-SMD) |
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293 | (18) |
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Chapter 16 Pharmacology of Store-Operated Calcium Entry Channels |
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311 | (14) |
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| Index |
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325 | |
Ashot Kozak is Associate Professor of Neuroscience, Cell Biology and Physiology in the Department of Biological Sciences at Wright State University in Dayton, Ohio. He received his PhD from Mount Sinai School of Medicine and did a postdoc at the University of California, Irvine. His research focuses upon cation channels expressed in leukocytes; Transient Receptor Potential (TRP) channels; ion channels involved in nociception.
