This second edition volume expands on the previous edition with updated methodologies and practical tips to overcome obstacles associated with experimentation pertaining to chemistry, biology, physiology, pathology, medical and dental sciences, and pharmacology of CCN proteins. The chapters in this book cover topics such as CCN4 immunofluorescence for tissue microarray; utilizing public molecular biological databases for CCN family research; the effects of CCN4 on pancreatic beta cell proliferation; gene expression analysis of CCNs; novel cell biological assays for measuring bone remodeling activities of CCN proteins; and the function of CCN2 in tubular epithelium cells with a focus on renal fibrogenesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, CCN Proteins: Methods and Protocols, Second Edition is a valuable resource for experienced CCN researchers looking for new approaches and novice researchers just starting out in the field of CCN research.
CCN Proteins - Cellular Communication Network Factors: Expanding their
Repertoire toward a New Concept.- Optimal CCN4 Immunofluorescence for Tissue
Microarray.- Cellular Fluorescence Imaging for the Evaluation of Bioactivity
of CCN Family Proteins- Imaging of Molecular Interaction between CCN Protein
and Its Binding Partners: An In Situ Proximity Ligation Assay of Interaction
Between CCN2 and Rab14 in Chondrocytes.- Western Blot Protocols for Analysis
of CCN Proteins and Fragments in Exosomes, Vesicle-free Fractions, and
Cells.- Comprehensive Method for Exosome Isolation and Proteome Analysis for
Detection of CCN Factors in/on Exosomes.- Evaluation of the Molecular
Interaction between CCN Protein and Its Binding Partners: A Solid-Phase
Binding Assay and Surface Plasmon Resonance.- Protocols for Screening
Peptides Binding to CCN Family Proteins and their Extended
Utility.- Transfection, Spinfection, Exofection, and Luciferase Assays for
Analysis of CCN Genes Expression Mechanism.- Analyses of the
Post-Transcriptional Regulation of CCN Genes: Approach to Multiple Steps of
CCN2 Gene Expression.- Protocol for CRISPR/Cas Genome Editing for
Investigating Cell Communication Network.- Utilizing Public Molecular
Biological Databases for CCN Family Research.- Evaluation of the Effects of
CCN4 on Pancreatic Beta Cell Proliferation.- Analysis of CCN Functions in
Liver Regeneration After Partial Hepatectomy.- The Evaluation of Meniscus
Regenerative Effects of LIPUS-Induced CCN Proteins: Induction by LIPUS of
CCN2 and Meniscus-Related Genes in Cultured Meniscus Cells and Meniscus
Tissues.- Analysis of Chemotactic Property of CCN2/CTGF in Intramembranous
Osteogenesis.- Novel Cell Biological Assays for Measuring Bone Remodeling
Activities of CCN Proteins.- Imaging Analysis of Neurotrophic Effects by CCN2
Protein in Neuronal Precursor Cells Derived from Human Induced Pluripotent
Stem Cells.- Gene Expression Analysis of CCNs Along with Odontoblastic
Differentiation In Vivo.- Angiogenesis Assays for the Analysis of CCN
Proteins.- Using the Bleomycin-Induced Model of Fibrosis to Study the
Contribution of CCN Proteins to Scleroderma Fibrosis.- Atomic Force
Microscopy-Based Measurements of Retinal Microvessel Stiffness in Mice with
Endothelial-Specific Deletion of CCN1.- Immunohistochemical Analysis of CCN2
in Experimental Fracture Healing Models.- Mouse Models of Tumor Bone
Metastasis and Invasion for Studying CCN Proteins.- Regulation of CCN2 and
Its Bioactivity by Advanced Glycation End Products.- Analysis of CCN4/WISP1
Effects on Joint Tissues Using Gain and Loss of Function
Approaches.- Generation of Conditional KO Mice of CCN2 and Its Function in
the Kidney.- Analysis of the Function of CCN2 in Tubular Epithelium Cells
with a Focus on Renal Fibrogenesis.
