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Cellular Spatial Characterization and Analysis [Kõva köide]

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  • Formaat: Hardback, 240 pages, kõrgus x laius: 254x178 mm, 47 Illustrations, color; 4 Illustrations, black and white
  • Sari: Methods in Molecular Biology
  • Ilmumisaeg: 20-Apr-2026
  • Kirjastus: Humana
  • ISBN-10: 1071650262
  • ISBN-13: 9781071650264
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Cellular Spatial Characterization and AnalysisSuurem pilt
  • Formaat: Hardback, 240 pages, kõrgus x laius: 254x178 mm, 47 Illustrations, color; 4 Illustrations, black and white
  • Sari: Methods in Molecular Biology
  • Ilmumisaeg: 20-Apr-2026
  • Kirjastus: Humana
  • ISBN-10: 1071650262
  • ISBN-13: 9781071650264
Teised raamatud teemal:
Püsilink: https://www.kriso.ee/db/9781071650264.html
Märksõnad:

This volume covers the latest advancements in staining techniques, and protocols and process data using various bioinformatic approaches. The chapters in this book are divided into broad themes of traditional immunohistochemistry, advanced spatial proteomics, and spatial genomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Cellular Spatial Characterization and Analysis is a valuable resource for any researchers looking to learn more about this developing field.

 

1.      High-Plex Tissue Imaging with Conventional Immunofluorescence
Platforms and Open-Source Software via Iterative Bleaching Extends
Multiplexity (IBEX).- Multiplex Immunofluorescence Staining of
Paraffin-Embedded Tissue: Coverslip Mounting of Tissue Sections Improves
Z-axis Optical Resolution in Widefield Microscopy.- Evaluation of Murine
Tissue Architecture and Cellular Spatial Relationships in Murine Tumor
Microenvironments Utilizing Multiplex Fluorescent Immunohistochemistry.-
Autostaining Immune Multiplex Immunohistochemistry Panels and Imaging
Analysis.- Fully Integrated Sample Preparation-Based Spatial Proteomic
Analysis.- Detailed Protocol for Expansion of Large Tissue Samples for
Expansion Microscopy.- Current Methods in CODEX High-Multiplex Microscopy to
Describe Spatial Relationships in the Tissue Microenvironment.- Mapping the
Tumor Microenvironment with Integrative Single-Cell RNA Sequencing and
Spatial Proteomics: Uncovering Mechanisms of Disease and Therapeutic
Resistance.- Single-Molecule Fluorescence In Situ Hybridization (smFISH) for
RNA Localization Relative to a DNA Locus in Bacteria.- A Protocol for Spatial
Transcriptomics using the 10x Genomics Visium Platform.- Highly Multiplexed
Single-Cell In Situ RNA and DNA Analysis by Consecutive Hybridization.- From
Raw Data to Biological Insights: A Practical Guide for Spatial
Transcriptomics Analysis in R and Python.