Combinatorial Biotechnology: Methods for Synthesis of Peptide, Oligonucleotide and Oligosaccharide Libraries and Applications covers methods used for synthesizing peptide, oligonucleotide, and oligosaccharide libraries. It presents the concepts of combinatorial biotechnology, detailing both solid phase and solution phase synthesis techniques, including modern biological methods such as in vitro and in vivo display libraries. Key topics include solid phase synthesis, parallel peptide synthesis, encoding technologies, deconvolution methods, and chemical and biological ligations. With chapters covering the chemical and biological synthesis of recombinant peptides and proteins, as well as the genetic reprogramming of the genetic code, this book offers a rich source of practical guidance.
The inclusion of advanced methods and illustrative examples makes it a unique and essential reference in the field. This book is an essential resource for undergraduate and postgraduate students in biochemical engineering, biotechnology, and biosciences, as well as industry professionals in R&D divisions. It equips readers with the latest techniques and applications in combinatorial biotechnology, addressing current challenges and advancing their research and development efforts.
1. Solid Phase synthesis
2. Solid phase peptide synthesis
3. Solid phase Oligonucleotide synthesis
4. Solid phase oligosaccharide synthesis
5. Parallel peptide synthesis on solid phase Parallel synthesis: microplate
method, Tea bag approach, Multi pin approach, SPOT synthesis, Light directed
parallel synthesis, parallel synthesis using micro fluidic devices, peptide
synthesis using peptide laser printer, cLIFT
6. Split only approach for peptide synthesis
7. Split and mix approach for peptide synthesis
8. Encoding Technologies Peptide encoding, Shaving, Oligonucleotide encoding,
PNA encoding, Binary encoding, Mass encoding, optical encoding using
fluorescent colloids, internal encoding, Physical encoding, electronic
encoding, Graphical encoding.
9. Solution phase synthesis Dendrimers, Polymer supported reagents, scavenger
resins, Fluorous reactants
10. Encoding in solution phase
11. Deconvolution methods Iterative deconvolution, Positional scanning,
Orthogonal deconvolution, Subtractive deconvolution, Bogus coin
deconvolution, Recursive deconvolution
12. Chemical Ligations Native Chemical ligation, Intein mediated ligation,
Staudinger ligation
13. Biological methods: In vivo Display Libraries Filamentous phage display,
lambda phage display, Bacterial cell surface display, Yeast cell surface
display, Baculovirus capsid display, Mammalian cell surface display
14. Biological methods: In vitro display libraries Ribosomal display, mRNA
display, CIS/CAD display, Plasmid display, In vitro compartmentalization
15. Genetic code reprogramming
Dr. Preeti Srivastava obtained PhD from Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Delhi in the area of corynebacterial plasmid biology. She did postdoctoral research work in the area of chromosome dynamics in Vibrio cholerae from Laboratory of Biochemistry and molecular biology, National Cancer Institute (NCI), National Institutes of Health (NIH), Bethesda, Maryland, USA. She has worked as Senior Scientist in Environmental Biotechnology Division, Indian Institute of Toxicology Research (CSIR), Lucknow before joining IIT Delhi in 2011. Currently, she is working as Professor, Department of Biochemical Engineering and Biotechnology, IIT Delhi. Her present research interest is in Bacterial genetics and Environmental Biotechnology. She has published several papers in Internationally reputed journals and proceedings of National and International conferences. She has been granted 4 patents and has filed three patent applications. She is the recipient of the prestigious Innovative Young Biotechnologist Award and TATA innovation fellowship from Department of Biotechnology, Ministry of Science and technology, Government of India.
