This third edition expands upon the previous two editions with new and updated methods presenting comprehensive, step-by-step protocols for recently developed techniques that were not included in the earlier volumes. Chapters feature contributions from the globally leading experts in the field, aiming to equip researchers with the practical tools necessary for successful CRISPR experimentation.
Authoritative and cutting-edge, CRISPR-Cas Methods, Volume 3 aims to be a useful and practical guide for researches to help further their study in this field.
Targeted base editing in rice using Target-AID.- Accelerated laboratory
evolution of conjugative plasmids for CRISPR delivery in bacteria.- A
SERS-signalled, CRISPR-Cas-powered bioassay for amplification-free and
anti-interference detection of SARS-CoV-2 in foods and environmental samples
using a single tube-in-tube vessel.- Genetic Engineering of Bacteriophage
Using CRISPR-Cas12a.- CRISPR-Cas12a-powered dual-mode biosensor for
ultrasensitive and cross-validating detection of pathogenic bacteria.-
Intronization of coding sequences for optimization of gene expression.-
Sensitive small molecule aptasensing-based on hybridization chain reaction
and CRISPR-Cas12a using a portable 3D-printed visualizer.- Highly efficient
genome editing in plants with the LbCas12a-RRV variant.- CRISPR-Cas13a-based
bacterial detection platform: Sensing pathogen Staphylococcus aureus in food
samples.- High efficiency Cas12a mediated A-to-G base editing in rice.- A
smartphone-based visual biosensor for CRISPR-Cas powered SARS-CoV-2
diagnostics.- Efficient single-cell cloning of genome-edited cultured human
cells.- Ultrasensitive pathogenic bacteria detection by a smartphone-read
G-quadruplex-based CRISPR-Cas12a bioassay.- A ratiometric fluorescent
biosensing platform for ultrasensitive detection of Salmonella typhimurium
via CRISPR-Cas12a and silver nanoclusters.- CRISPR-Cas12a-based aptasensor
for sensitive and selective ATP detection.- A portable, visual and dual-mode
biosensor for ultrasensitive and self-validating detection of foodborne
pathogenic bacteria based on CRISPR-Cas9 system.- CRISPR-Cas12-based PCV2
viral detection.- Surface-enhanced Raman scattering -based CRISPR-Cas assay
on microfluidic paper analytical devices for supersensitive detection of
pathogenic bacteria in foods.- CRISPR-Cas in woody perennial plants: methods,
efficiency, applications, and challenges to creating commercial varieties
with high ecological and economic value.- A Unified Protocol for Genome
Editing in Monocot and Dicot Plants Using a Transposon-Associated TnpB
System.- CRISPR-Cas12a-Based DNA Detection for Rapid Diagnosis of Wheat Blast
Disease.
