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Detection of Plant Viruses: Advanced Techniques [Kõva köide]

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  • Formaat: Hardback, 304 pages, kõrgus x laius: 254x178 mm, 17 Illustrations, color; 7 Illustrations, black and white; XIV, 304 p. 24 illus., 17 illus. in color., 1 Hardback
  • Sari: Springer Protocols Handbooks
  • Ilmumisaeg: 28-Feb-2025
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071643894
  • ISBN-13: 9781071643891
Teised raamatud teemal:
  • Kõva köide
  • Hind: 187,67 €*
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  • Formaat: Hardback, 304 pages, kõrgus x laius: 254x178 mm, 17 Illustrations, color; 7 Illustrations, black and white; XIV, 304 p. 24 illus., 17 illus. in color., 1 Hardback
  • Sari: Springer Protocols Handbooks
  • Ilmumisaeg: 28-Feb-2025
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071643894
  • ISBN-13: 9781071643891
Teised raamatud teemal:
This volume provides researchers and students with an introduction to the identification and transmission of plant viruses as well as providing them with practical methodologies that demonstrate the detection of plant viruses through immunological and molecular methods.





Authoritative and cutting-edge, Detection of Plant Viruses: Advanced Techniques aims to ensure successful results in the further study of this vital field.
Introduction.- Identification Of Plant Viruses Based On Symptoms.-
Mechanical Inoculation Of Plant Viruses.- Transmission Of Plant Virus By
Grafting.- Isolation And Identification Of Virus Strains.- Determination Of
Dilution End Point Of Viruses.- Thermal Inactivation Of Plant Viruses.-
Bioassays Of Plant Viruses.- Direct Enzyme Linked Immuno Sorbent Assay
(Elisa) For Detection Of Plant Viruses.- Direct Antigen Coated (Dac) Enzyme
Linked Immuno Sorbent Assay (Elisa) For Detection Of Plant Viruses.- Double
Antibody Sandwich Enzyme Linked Immuno Sorbent Assay (Elisa) For Detection Of
Plant Viruses.- Electron Microscopy For Plant Viral Detection.- Extraction Of
High-Quality Genomic Dna From Plants Using Modified Ctab-Based Method.-
Extraction Of High-Quality Genomic Rna Using Modified Trizol Methods.-
Extraction Of High-Quality Genomic Rna Using Modified Ctab Method.-
Extraction Of High-Quality Genomic RNA Using  Modified Kit Method.- Gel
Electrophoresis For Visualisation Of Nucleic Acid.- Polyacrylamide Gel
Electrophoresis For Visualisation Of Nucleic Acid.- Measurement Of RNA/DNA
Concentration Using Spectrophotometer.- Polymerase Chain Reaction (Pcr) For
Virus Detection.- Reverse Transcription- Polymerase Chain Reaction (Rt-Pcr)
For RNA Viruses.- RT-Nested Pcr For Plant Virus Detection.- Real Time PCR
(qPCR) For Plant Virus Detection And Expression Analysis.- MULTIPLEX PCR IN
PLANT VIRUS DETECTION.- COLONY PCR In Plant Virology.- APPLICATION OF DIGITAL
PCR (dPCR) IN PLANT VIRUS DETECTION.- Immuno-Capture Polymerase Chain
Reaction (IC-PCR) In Detection Of Plant Viruses.- REVERSE TRANSCRIPTION LOOP
MEDIATED ISOTHERMAL AMPLIFICATION (RT-LAMP) FOR PLANT VIRAL DETECTION.-
RECOMBINASE POLYMERASE AMPLIFICATION FOR VIRAL DETECTION.- DETECTION OF PLANT
VIRUSES BY CRISPR-Cas13a SYSTEM.- DETECTION OF PLANT VIRUSES THROUGH
RNA-Seq.- PREPARATION OF SEQUENCING LIBRARY FOR OXFORD NANOPORE MINION
SEQUENCING.- REDUCED REPRESENTATION METHYLATION SEQUENCING (RRMS) FOR PLANT
VIRUSES.- REDUCED REPRESENTATION BISULFITE SEQUENCING (RRBS) FOR PLANT
VIRUSES.- REMOTE SENSING FOR VIRUS DETECTION.- ELECTROCHEMICAL METHODS FOR
VIRUS DETECTION.- NEXT-GENERATION FLOW CYTOMETRY METHODS FOR PLANT VIRUS
DETECTION.- SURFACE PLASMON RESONANCE FOR VIRUS DETECTION IN PLANTS.- IN VIVO
SYNTHESIS AND PURIFICATION OF DOUBLE STRANDED RNA (dsRNA) for RNA
INTERFERANCE (RNAi).