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DNA Microarrays: Gene Expression Applications [Kõva köide]

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  • Formaat: Hardback, 149 pages, kõrgus x laius x paksus: 235x159x13 mm, kaal: 363 g, 20 figs.
  • Sari: Principles and Practice
  • Ilmumisaeg: 31-Jul-2001
  • Kirjastus: Springer-Verlag Berlin and Heidelberg GmbH & Co. K
  • ISBN-10: 3540415076
  • ISBN-13: 9783540415077
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  • Formaat: Hardback, 149 pages, kõrgus x laius x paksus: 235x159x13 mm, kaal: 363 g, 20 figs.
  • Sari: Principles and Practice
  • Ilmumisaeg: 31-Jul-2001
  • Kirjastus: Springer-Verlag Berlin and Heidelberg GmbH & Co. K
  • ISBN-10: 3540415076
  • ISBN-13: 9783540415077
Teised raamatud teemal:
This complete and practical manual on expression measurement using DNA arrays covers the existing methods (from nylon macroarrays to oligonucleotide chips) and includes detailed protocols. It has been written by practising scientists who have experienced the difficulties involved in actually using microarrays, and provides helpful advice and hints on setting up these powerful but sometimes tricky methods. Software, data mining procedures and probable future developments, which should be useful to any practising scientist interested in expression measurement, are also covered in this book. It also provides detailed protocols as well as many helpful hints to achieve experimental success and to avoid pitfalls.
DNA Arrays for Expression Measurement: An Historical Perspective Bertrand R. Jordan Introduction 3(1) The Forerunners: Colony Filters and Dot Blots 3(2) ``High-Density Filters 5(2) Colony Filters and Expression Analysis (Qualitative) 7(1) Quantifying Expression with High-Density Filters 7(3) Miniaturisation: cDNA Microarrays 10(1) Miniaturisation: Oligonucleotide Chips 10(1) Data Acquisition, Storage and Mining 11(1) A (Provisional) Conclusion 11(4) References 12(3) Expression Profiling with cDNA Microarrays: A Users Perspective and Guide Sean Grimmond Andy Greenfield Introduction 15(1) Expression Profiling with cDNA Microarrays: The Basics 15(2) Production of cDNA Microarrays 17(10) Conclusions 27(1) Protocols 28(9) References 33(4) cDNA Microarrays on Nylon Membranes with Enzyme Colorimetric Detection Konan Peck Yuh-Pyng Sher Introduction 37(1) Fabrication of Microarrays on Nylon Membranes 37(3) Enzyme Colorimetric Detection 40(1) Characteristics of the Microarray/CD System 41(5) Data Processing in the Microarray/CD System 46(3) Protocol 1: Preparation of cDNA Targets for Microarray Fabrication 49(1) Protocol 2: cDNA Complex Probe Labeling 50(1) Protocol 3: Array Hybridization and Color Development 51(1) Protocol 4: Modified Catalyzed Reporter Deposition (CARD) Method 52(5) References 53(4) cDNA Macroarrays and Microarrays on Nylon Membranes with Radioactive Detection Beatrice Loriod Genevieve Victorero Catherine Nguyen Introduction 57(1) Manufacture of Nylon Arrays 58(7) Labelling and Hybridisation with Complex Probes from Total RNA 65(2) Signal Analysis 67(6) Protocol 1: Array Preparation 73(4) Protocol 2: RNA Preparation 77(1) Protocol 3: Labelling 78(2) Protocol 4: Hybridisation 80(2) Protocol 5: Data Processing 82(5) References 84(3) Oligonucleotide Chips for Expression Analysis: Principles and Practical Procedures Pierre Casellas Annick Peleraux Sylvaine Galiegue Introduction 87(1) Principles 87(9) Bioinformatics 96(4) Conclusion 100(6) References 101(5) Gene Expression Data Mining and Analysis Alvis Brazma Alan Robinson Jaak Vilo Introduction 106(1) From Raw Data to Gene Expression Matrix 107(3) Gene Expression Matrix Analysis 110(9) Expression Profiler 119(1) Applications 120(2) From Gene Expression Data to Gene Regulatory Networks 122(3) Conclusions 125(8) References 126(7) Future Trends in the Use of DNA Arrays for Expression Measurement Bertrand R. Jordan Introduction 133(1) Future Chips: How Complex? 133(1) From ``Clone-Based to ``Sequence-Based Arrays 134(2) From ``Home-Made Microarrays to Ready-Made Chips 136(1) From ``Stand Alone Array to Integrated ``Lab-on-a-Chip 136(1) From Fluorescent Labelling to Electrical Detection 137(1) Progress Towards More Sophisticated Data Interpretation and (Maybe) a Unified Expression Repository 137(1) Expression Measurement Is Here to Stay 138(1) References 138(1) Subject Index 139