This volume details the importance of bacteria in and around the human body, highlighting the bacteria that is harmless and pathogenic bacteria. Chapters guide readers through protocols on different bacteria’s providing a better understand on they operate and how people can protect ourselves. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Dynamics of Bacteria-Mucus Interactions aims to be comprehensive guide for researchers in the field.
Breaking Barriers: A Protocol to Investigate Intestinal Pathogen
Adhesion, Invasion and Translocation Through In Vitro, In Vivo, and Imaging
Technologies.- Quantitative assessment of MUC5AC and Helicobacter pylori
adhesion to the gastric tissue on the model of Cavia porcellus.- Growth and
Polarization of A549 Human Alveolar Epithelial Cells as a Model to Study
Biofilm Formation in Acinetobacter baumannii.- Mucins as inhibitors against
bacterial adhesion to epithelial cells.- Profiling of anti-glycan IgA and IgG
in oral fluid using a glycan microarray.- Assays for Anti-Helicobacter pylori
Effects of Lactic Acid Bacteria Secretions.- Assessing the relative
affinities of bacterial lectins for sugars.- Investigating bacterial invasion
of highly differentiated human bronchial epithelial cell barriers.- Methods
of a reproducible mouse model of Clostridioides difficile infection to
investigate novel bacterial based therapies.- Purification of Type IV and
Type VI lipoteichoic acids from Gram-positive mucosal pathogens via a
two-step hot water-phenol/triethylammonium acetate extraction method.-
Microbial lectins targeting mucus: A glycobioinformatic approach.- Exploring
the O-glycomic Degradome Using Natural Mucin Libraries.- Detection of
Bacterial Adhesion to Mucus Slot-Blotted on Polyvinylidene Difluoride (PVDF)
Membrane.- Quantification of -Lactamase Inhibition using a Luminescent
Whole-Cell Biosensor.- Mucins and bacterial interactions.- Measuring and
Analyzing Bacterial Movement in Mucus.- Adhesion of Pseudomonas aeruginosa to
human outer layer airway mucins.
