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Enzyme Nanoarchitectures: Enzymes Armored with Graphene, Volume 609 [Kõva köide]

Volume editor (Departments of Chemistry and of Molecular and Cell Biology, University of Connecticut, Connecticut, USA)
  • Formaat: Hardback, 419 pages, kõrgus x laius: 229x152 mm, kaal: 910 g
  • Sari: Methods in Enzymology
  • Ilmumisaeg: 24-Sep-2018
  • Kirjastus: Academic Press Inc
  • ISBN-10: 0128152400
  • ISBN-13: 9780128152409
Teised raamatud teemal:
  • Formaat: Hardback, 419 pages, kõrgus x laius: 229x152 mm, kaal: 910 g
  • Sari: Methods in Enzymology
  • Ilmumisaeg: 24-Sep-2018
  • Kirjastus: Academic Press Inc
  • ISBN-10: 0128152400
  • ISBN-13: 9780128152409
Teised raamatud teemal:

Enzymes Conjugated to Graphene, Volume 609 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on Enzyme immobilization, Detection of Urea, Enzyme immobilization Enzyme immobilization, PAMAM dendrimer modified reduced graphene oxide post functionalized by horseradish peroxidase for biosensing H2O2, HRP immobilized for LEV detection, Enzyme immobilization, Graphene biocatalysts, Enzyme immobilization, Interactions, Enzyme immobilization, GQD, Enzyme Immobilization, and Enzyme immobilization on functionalized graphene oxide nanosheets.

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods of Enzymology series
  • Updated release includes the latest information on the enzymes conjugated to graphene
Contributors xi
Preface xv
1 Interlocking Enzymes in Graphene-Coated Cellulose Paper for Increased Enzymatic Efficiency
1(22)
Melissa R. Limbacher
Megan K. Puglia
Caterina M. Riccardi
Challa V. Kumar
1 Introduction
2(8)
2 Materials and Methods
10(9)
3 Concluding Remarks
19(4)
Acknowledgments
20(1)
References
20(3)
2 Enzyme Multilayers on Graphene-Based FETs for Biosensing Applications
23(24)
Christina Bliem
Esteban Piccinini
Wolfgang Knoll
Omar Azzaroni
1 Introduction
24(3)
2 rGO-Based FETs
27(5)
3 Enzyme Immobilization
32(4)
4 Enzymatic Biosensors
36(6)
5 Summary and Conclusions
42(5)
Acknowledgments
42(1)
References
42(5)
3 Stabilization of Laccase Through Immobilization on Functionalized GO-Derivatives
47(36)
Alexandra V. Chatzikonstantinou
Elena Gkantzou
Dimitrios Gournis
Michaela Patila
Haralambos Stamatis
1 Introduction
48(6)
2 Synthesis of Nanomaterials
54(2)
3 Immobilization of Laccase on GO-Derivatives
56(8)
4 Characterization of the Immobilized Biocatalysts
64(8)
5 Stability and Reusability of Nanobiocatalysts
72(5)
6 Conclusion
77(6)
Acknowledgments
77(1)
References
78(5)
4 Synthesis, Characterization, and Applications of Nanographene-Armored Enzymes
83(60)
Maryam Khan
Qayyum Husain
Shamoon Asmat
1 Introduction
85(5)
2 Facile Synthesis of Enzyme-Graphene-Armored Nanocomposites as Biocatalysts
90(1)
3 Immobilization of the Enzyme Onto Graphene Anchored Nanocomposites
90(40)
4 Summary and Future Outlook
130(13)
Acknowledgments
133(1)
Funding
133(1)
References
133(9)
Further Reading
142(1)
5 PAMAM Dendrimer Modified Reduced Graphene Oxide Postfunctionalized by Horseradish Peroxidase for Biosensing H2O2
143(28)
Sheela Berchmans
Manju Venkatesan
Chirnajeevi Srinivasa Rao Vusa
Palaniappan Arumugam
1 Introduction
144(2)
2 Functionalization of Graphene (or) RGO by Noncovalent Interactions
146(2)
3 Covalent Functionalization of Graphene, RGO
148(1)
4 Graphene in Biosensing
149(2)
5 Synthesis of Carrot Extract Reduced Graphene Oxide
151(5)
6 Functionalization of Graphene by PAMAM Dendrimers via Electrochemical Grafting for Enzymatic Sensing Application
156(4)
7 Postfunctionalization of GCE/Ct-RGO-PAMAM by the Horseradish Peroxidase Enzyme
160(1)
8 Application of HRP-Modified GCE/Ct-RGO-PAMAM-GA-HRP for H2O2 Sensing
161(1)
9 Application of HRP-Modified GCE/Ct-RGO-PAMAM-GA-HRP for H2O2 Sensing in Serum
162(1)
10 Conclusions
163(8)
Acknowledgments
164(1)
References
164(7)
6 Preparation, Characterization, and Application of Enzyme Nanoparticles
171(26)
Neelam Yadav
Jagriti Narang
Anil Kumar Chhillar
Chandra S. Pundir
1 Introduction
172(2)
2 Experimental
174(6)
3 Immobilization of ENPs
180(4)
4 Stability and Reusability
184(2)
5 Applications of ENPs
186(6)
6 Detailed Procedure for the Development of HbNPs-Based Biosensors
192(2)
7 Conclusions and Future Perspective
194(3)
Conflict of Interest
194(1)
References
194(2)
Further Reading
196(1)
7 Encapsulation of Microorganisms, Enzymes, and Redox Mediators in Graphene Oxide and Reduced Graphene Oxide
197(24)
Orr Schlesinger
Lital Alfonta
1 Introduction
198(3)
2 Methods
201(13)
3 Concluding Remarks
214(7)
Acknowledgments
215(1)
Funding
215(1)
References
215(6)
8 Chemical and Biochemical Approach to Make a Perfect Biocatalytic System on Carbonaceous Matrices
221(26)
Paulina Bolibok
Katarzyna Roszek
Marek Wisniewski
1 Introduction
222(14)
2 Methods
236(4)
3 Summary and Perspectives
240(7)
References
241(6)
9 Immobilization of a Mesophilic Lipase on Graphene Oxide: Stability, Activity, and Reusability Insights
247(26)
Nalok Dutta
Malay K. Saha
1 Introduction
248(3)
2 Materials and Methods
251(5)
3 Enzyme Aggregation Analysis by Dynamic Light Scattering (DLS) Studies
256(2)
4 Results
258(7)
5 Discussion
265(4)
6 Conclusion
269(4)
Acknowledgment
269(1)
Funding
269(1)
References
269(3)
Further Reading
272(1)
10 A Simple Flow Reactor for Continuous Synthesis of Biographene for Enzymology Studies
273(20)
Megan K. Puglia
Murali Anuganti
Yao Lin
Challa V. Kumar
1 Introduction
274(1)
2 Continual Synthesis of High-Quality Biographene
275(4)
3 Preparation and Enzymology Applications
279(1)
4 Methods
280(9)
5 Conclusions
289(4)
Acknowledgment
290(1)
References
290(3)
11 Enzyme-Graphene Platforms for Electrochemical Biosensor Design With Biomedical Applications
293(42)
Luminita Fritea
Mihaela Tertis
Robert Sandulescu
Cecilia Cristea
1 Introduction
295(3)
2 Electrochemical Biosensors: General Consideration
298(10)
3 Graphene and Graphene Compounds Used for Biosensors Design
308(1)
4 Enzymes Used for Electrochemical Biosensors Based on Graphene
309(17)
5 Future Trends
326(1)
6 Conclusions
327(8)
Acknowledgments
327(1)
References
328(7)
12 Graphene Quantum Dots: Synthesis and Applications
335(20)
Ankarao Kalluri
Debika Debnath
Bhushan Dharmadhikari
Prabir Patra
1 Introduction
336(1)
2 Graphene Quantum Dots
337(6)
3 Methods
343(2)
4 Characterization of GQDs
345(7)
5 Applications of the GQDs
352(1)
6 Summary and Conclusions
352(3)
Acknowledgments
352(1)
References
353(2)
13 Shielding of Enzymes on the Surface of Graphene-Based Composite Cellular Foams Through Bioinspired Mineralization
355(16)
Yiying Sun
Jingjing Zhao
Jiafu Shi
Shaohua Zhang
Zhongyi Jiang
1 Introduction
356(1)
2 Preparation of GCCFs
357(5)
3 Structure Control of GCCFs
362(3)
4 Activity and Stabilities of GCCFs
365(3)
5 Concluding Remarks
368(3)
Acknowledgments
369(1)
References
369(2)
14 Enzyme Immobilization on Functionalized Graphene Oxide Nanosheets: Efficient and Robust Biocatalysts
371
Asieh Soozanipour
Asghar Taheri-Kafrani
1 Introduction
373(2)
2 Synthesis of Graphene-Based Nanomaterials
375(3)
3 Modification of Graphene-Based Nanomaterials
378(5)
4 Application of Graphene-Based Nanomaterials for Enzyme Immobilization
383(6)
5 Overview of Our Strategy to Fabricate a Novel GO Nanosheet for Enzyme Immobilization
389(7)
6 Challenges and Concluding Remarks
396
Acknowledgment
397(1)
References
397(6)
Further Reading
403
Challa Vijaya Kumar, PhD is a senior professor at the University of Connecticut and has more than 40 years of research experience in interdisciplinary research areas including biological chemistry, physical chemistry, organic chemistry, photochemistry, biochemistry and material chemistry. He has more than 158 peer-reviewed publications and several book chapters in internationally reputed journals. He has been actively working in the area of bio-related two-dimensional materials for nearly 25 years and has published 10 papers on graphene related topics in the last 3 years. More importantly, his research lab pioneered the synthesis of graphene in aqueous conditions using proteins as exfoliating agents.