This detailed book provides a state-of-the-art compilation of protocols relevant for the experimental study of cholestasis, each written by internationally recognized research teams that have built up long-standing expertise regarding one or more aspects of experimental cholestasis research. The first half of the collection examines methods targeted towards studying established and new mechanisms of cholestasis, while the second half explores experimental models and tools to investigate mechanisms of cholestasis and/or to predict cholestatic properties of drugs. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Experimental Cholestasis Research serves as an ideal guide for basic and applied researchers pursuing this vital area of research in pharmacology and toxicology.
Mechanisms of Drug-Induced Cholestasis.- LC-MS/MS Analysis of Bile Acids
in In Vitro Samples.- Pipe-3D: A Pipeline Based on Immunofluorescence, 3D
Confocal Imaging, Reconstructions, and Morphometry for Biliary Network
Analysis in Cholestasis.- Vesicle-Based and Hepatocyte-Based Assays for
Identification of Drug Candidates Inhibiting BSEP Function.- The C-DILI
Assay: An Integrated In Vitro Approach to Predict Cholestatic
Hepatotoxicity.- In Vivo Measurement of Hepatic Drug Transporter Inhibition
with Radiolabeled Bile Acids.- Measuring the Impact of Bile Acids on the
Membrane Order of Primary Hepatocytes and Isolated Mitochondria by
Fluorescence Imaging and Spectroscopy.- The Role and Study of Mitochondrial
Impairment and Oxidative Stress in Cholestasis.- Measuring Apoptosis and
Necrosis in Cholestatic Liver Injury.- Necrosome Formation and Necroptosis in
Experimental Cholestasis.- Proteomics in Primary Biliary Cholangitis.-
Transcriptomics Analysis of Cholestatic Compounds In Vitro.- Systems
Microscopy of Stress Response Pathways in Cholestasis Research.- Mouse Models
for Diseases in the Cholangiocyte Lineage.- Common Bile Duct Ligation as
Model for Secondary Biliary Cirrhosis.-
3,5-Diethoxycarbonyl-1,4-Dihydrocollidine Diet: A Rodent Model in Cholestasis
Research.- A Rotavirus-Induced Mouse Model to Study Biliary Atresia and
Neonatal Cholestasis.- Zebrafish as a Model to Study Cholestatic Liver
Diseases.- Set-Up and Use of HepaRG Cells in Cholestasis Research.-
Organotypic 3D HepaRG Liver Model for Assessment of Drug-Induced
Cholestasis.- Establishment of Sandwich Cultures of Primary Human
Hepatocytes.- Detection of Drug-Induced Cholestasis Potential in
Sandwich-Cultured Human Hepatocytes.- Ex Vivo Model in Cholestasis Research.-
Patient-Derived Organoids from Human Bile: An In Vitro Method to Study
Cholangiopathies.- Use of Biliary Organoids in Cholestasis Research.- In
Silico Approaches to Predict Drug-Transporter Interaction Profiles: Data
Mining, Model Generation, and Link to Cholestasis.
