This book focuses on the development of functional nucleic acids and their applications of molecular detection methods in the context of food safety. The book has been expanded to 24 chapters based on the first edition including five newly-added chapters, of which involving the systematic introduction of currently-fashionable functional nucleic acid elements, the basic mechanism of diverse amplification technologies, and the novel design strategies of detection methods from the latest research findings for food safety. Offering a comprehensive overview of nucleic acids detection method in food safety for professionals and members of the public interested in this area.
1. Introduction: A Brief Guide to Targets and Strategies of Functional
Nucleic Acids Detection in Food Safety.-
2. Adaptable Methods to Extract
Nucleic Acid Targets and Evaluate Quality.-
3. Basic Rules in Nucleic
Acid-Mediated Amplification and Hybridization Methods in Food Safety
Detection: A Review.-
4. Modified nucleic acids.-
5. Reference Materials: A
Golden Criterion in Nucleic Acid Identification.-
6. Functional Nucleic Acid
Enzyme.- 7. Reference Gene: In-Species Universality Versus Between-Species
Uniquity.- 8. Super PCR.-
9. PCR-Based Technologies for Identifying Unknown
Gene Sequences.-
10. Triplex Nucleic Acids.-
11. A Developed Accurate Digital
PCR Detection Technology in Food Safety.-
12. Development of Accurate Nucleic
Acid Detection Technology for Target Quantification.-
13. Recent Progress in
High-Throughput Detection Technology for Food Safety.-
14. Detecting Targets
Without Thermal Cycling in Food: Isothermal Amplification and Hybridization.-
15. Cell System-Based Biosensors for Food Safety.-
16. Aptamers and Their
Application.-
17. Lateral Flow Nucleic Acid Biosensors.-
18. Nucleic Acid
Biosensors for Food Safety.-
19. PCR Methods for Detecting GM Crops and Food
in Agriculture and the Food Chain: A Review.- 20. The Identification and
Detection Technology of Research in Microorganisms Including Living or Dead
Bacteria.-
21. Characterization of Microbial Diversity in Food Chain: A
Molecular Review.-
22. Identification and Assessment of Heavy Metal Pollution
Using Nucleic Acid-Mediated Technologies.-
23. Genotoxicity Detection at the
Molecular Level in Food Safety Assessment: Conventional Methods and
Developments.-
24. A Molecular Review of the Detection of Specific Nucleic
Acids by Amplification and Hybridization Characterization of Microbial
Diversity in the Food Chain: A Molecular Review.
Wentao Xu obtained his B.S. and Ph.D. degrees from China Agricultural University in 2001 and 2006. After his postdoctoral research at China Agricultural University, he Page 10 of 10 joined the faculty at China Agricultural University. He is now an associate professor in the Collage of Food Science and Nutritional Engineering at China Agricultural University. His research interest is functional nucleic acids biosensors, precise toxicological detection technology and food safety risk assessment.
