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Gap Junction Protocols 1st ed. 2016 [Kõva köide]

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  • Formaat: Hardback, 235 pages, kõrgus x laius: 254x178 mm, kaal: 6221 g, 34 Illustrations, color; 11 Illustrations, black and white; XIII, 235 p. 45 illus., 34 illus. in color., 1 Hardback
  • Sari: Methods in Molecular Biology 1437
  • Ilmumisaeg: 21-May-2016
  • Kirjastus: Humana Press Inc.
  • ISBN-10: 149393662X
  • ISBN-13: 9781493936625
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  • Formaat: Hardback, 235 pages, kõrgus x laius: 254x178 mm, kaal: 6221 g, 34 Illustrations, color; 11 Illustrations, black and white; XIII, 235 p. 45 illus., 34 illus. in color., 1 Hardback
  • Sari: Methods in Molecular Biology 1437
  • Ilmumisaeg: 21-May-2016
  • Kirjastus: Humana Press Inc.
  • ISBN-10: 149393662X
  • ISBN-13: 9781493936625
Teised raamatud teemal:
Presenting state-of-the-art protocols to study gap junctions, this detailed book first focuses on the use of methods and tools to investigate the different aspects of connexin expression and gap junction regulation. The second part of the volume describes several methods to probe gap junction functionality as such. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Gap Junction Protocols is intended for basic and applied researchers in the area of biomedical and life sciences, both in academic and industrial settings.
Preface v
Contributors xi
1 Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction
1(20)
Michael Maes
Joost Willebrords
Sara Crespo Yanguas
Bruno Cogliati
Mathieu Vinken
2 DNA Methylation Analysis of Human Tissue-Specific Connexin Genes
21(16)
Xiaoming Liu
Canxia Xu
3 Detection of Connexins in Liver Cells Using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis
37(18)
Joost Willebrords
Michael Maes
Sara Crespo Yanguas
Bruno Cogliati
Mathieu Vinken
4 Immunohisto-and Cytochemistry Analysis of Connexins
55(16)
Bruno Cogliati
Michael Maes
Isabel Veloso Alves Pereira
Joost Willebrords
Tereza Cristina Da Silva
Sara Crespo Yanguas
Mathieu Vinken
5 Small Interfering RNA-Mediated Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells
71(12)
Miranda E. Good
Daniela Begandt
Leon J. DeLalio
Scott R. Johnstone
Brant E. Isakson
6 Generation and Use of Trophoblast Stem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancy and Labor
83(14)
Mark Kibschull
Stephen J. Lye
Oksana Shynlova
7 Identification of Connexin43 Phosphorylation and S-Nitrosylation in Cultured Primary Vascular Cells
97(16)
Alexander W. Lohman
Adam C. Straub
Scott R. Johnstone
8 Preparation of Gap Junctions in Membrane Microdomains for Immunoprecipitation and Mass Spectrometry Interactome Analysis
113(20)
Stephanie Fowler
Mark Akins
Steffany A. L. Bennett
9 Scrape Loading/Dye Transfer Assay
133(12)
Pavel Babica
Iva Sovadinova
Brad L. Upham
10 Microinjection Technique for Assessment of Gap Junction Function
145(10)
Michael D. Fridman
Jun Liu
Yu Sun
Robert M. Hamilton
11 Electroporation Loading and Dye Transfer: A Safe and Robust Method to Probe Gap Junctional Coupling
155(16)
Elke Decrock
Marijke De Bock
Diego De Baere
Delphine Hoorelbeke
Nan Wang
Luc Leybaert
12 Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro
171(10)
Maria Kuzma-Kuzniarska
Clarence Yapp
Philippa A. Hulley
13 Tracking Dynamic Gap Junctional Coupling in Live Cells by Local Photoactivation and Fluorescence Imaging
181(12)
Song Tang
Wen-Hong Li
14 A Functional Assay to Assess Connexin 43-Mediated Cell-to-Cell Communication of Second Messengers in Cultured Bone Cells
193(10)
Joseph P. Stains
Roberto Civitelli
15 Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Method for Studying Gap Junctions and Hemichannels
203(10)
Jegan Iyyathurai
Bernard Himpens
Geert Bultynck
Catheleyne D'hondt
16 Establishment of the Dual Whole Cell Recording Patch Clamp Configuration for the Measurement of Gap Junction Conductance
213(20)
Richard D. Veenstra
Index 233