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Gap Junction Protocols Softcover reprint of the original 1st ed. 2016 [Pehme köide]

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  • Formaat: Paperback / softback, 235 pages, kõrgus x laius: 254x178 mm, kaal: 4801 g, 34 Illustrations, color; 11 Illustrations, black and white; XIII, 235 p. 45 illus., 34 illus. in color., 1 Paperback / softback
  • Sari: Methods in Molecular Biology 1437
  • Ilmumisaeg: 26-May-2018
  • Kirjastus: Humana Press Inc.
  • ISBN-10: 1493981102
  • ISBN-13: 9781493981106
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  • Formaat: Paperback / softback, 235 pages, kõrgus x laius: 254x178 mm, kaal: 4801 g, 34 Illustrations, color; 11 Illustrations, black and white; XIII, 235 p. 45 illus., 34 illus. in color., 1 Paperback / softback
  • Sari: Methods in Molecular Biology 1437
  • Ilmumisaeg: 26-May-2018
  • Kirjastus: Humana Press Inc.
  • ISBN-10: 1493981102
  • ISBN-13: 9781493981106
Teised raamatud teemal:
Presentingstate-of-the-art protocols to study gap junctions, this detailed book firstfocuses on the use of methods and tools to investigate the different aspects ofconnexin expression and gap junction regulation. The second part of the volumedescribes several methods to probe gap junction functionality as such. Writtenin the highly successful Methods in Molecular Biology series format, chaptersinclude introductions to their respective topics, lists of the necessarymaterials and reagents, step-by-step and readily reproducible laboratoryprotocols, and key tips on troubleshooting and avoiding known pitfalls. 

Authoritativeand practical, Gap Junction Protocols is intended for basic and appliedresearchers in the area of biomedical and life sciences, both in academic andindustrial settings.
Analysis of Liver Connexin Expression
Using Reverse Transcription Quantitative Real-Time Polymerase Chain
Reaction.- DNA Methylation Analysis of Human
Tissue-Specific Connexin Genes.- Detection of Connexins in Liver Cells Using
Sodiumdodecylsulfate Polyacrylamide Gel Electrophoresis and Immunoblot
Analysis.- Immunohisto- and
Cytochemistry Analysis of Connexins.- Small Interfering RNA-Mediated
Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle
Cells.- Generation and Use of Trophoblast
Stem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancy
and Labor.- Identification of Connexin43
Phosphorylation and S-Nitrosylation
in Cultured Primary Vascular Cells.- Preparation of Gap Junctions in
Membrane Microdomains for Immunoprecipitation and Mass Spectrometry
Interactome
Analysis.- Scrape Loading/Dye Transfer Assay.- Microinjection Technique for
Assessment of Gap
Junction Function.- Electroporation Loading and Dye
Transfer: A Safe and Robust Method to Probe Gap Junctional Coupling.- Using
Fluorescence Recovery After Photobleaching to Study Gap Junctional
Communication In Vitro.- Tracking Dynamic Gap Junctional Coupling in Live
Cells by Local
Photo-Activation and Fluorescence Imaging.- A Functional Assay to Assess
Connexin43-Mediated Cell-to-Cell Communication of
Second Messengers in Cultured Bone Cells.- Calcium Wave Propagation Triggered
by Local Mechanical Stimulation as a Method
for Studying Gap Junctions and Hemichannels.- Establishment of the Dual Whole
Cell Recording Patch Clamp Configuration for
the Measurement of Gap Junction Conductance.