Comprising 23 chapters by molecular biologists who have either invented new methods or have extensive experience in applying them, this state-of-the-art review-cum-laboratory manual focuses on progress made in DNA sequencing with the recently developed tool of RNA interference (RNAi). Sohail (biochemistry, U. of Oxford) explains that such gene silencing methods, in which specific genes are "knocked out" in order to study which functions are lost, help elucidate naturally- occurring processes in plant and mammalian cells. In his contribution, the editor discusses his specialty area of the design strategies of gene silencing reagents. Other contributors treat applications for creating animal models for studying human and plant diseases. Chapters provide step-by-step protocols for experimental procedures. Annotation ©2004 Book News, Inc., Portland, OR (booknews.com)
Gene Silencing by RNA Interference: Technology and Application provides background in the field and describes methods and protocols for gene silencing. It offers a general introduction to the subject of gene silencing and RNA interference and focuses on technical details of the various methods of producing siRNAs and other RNAi tools. The final chapters of the book detail the application of RNAi to dissect gene function in a number of biological systems, including cell-free systems, cultured cells, and whole organisms. This book is a comprehensive guide to gene silencing by RNA interference methods for beginners and experienced professionals in the field.
