This detailed volume examines a wide range of disciplines involving genome assembly and its uses. Covering a diversity of taxa including bacteria, archaea, viruses, animals, plants, and fungi, the book demonstrates the similarities and differences in genome assembly methods and biological differences across those groups, while also describing the latest advances in modern sequencing strategies as well as techniques in the rapidly growing fields of meta-genomics and pan-genomics. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail to ensure successful results in new research.
Authoritative and practical, Genome Assembly serves as an ideal guide for researchers pursuing genome analysis, a highly active area of global biosciences research.
DNA Sequencing and the Third-Generation Sequencing Revolution.- Methods
for Chromosome-Scale Haplotype Reconstruction.- Genome Assembly by a
Non-Bioinformatician: Case Study of a Small Predatory Natural Enemy Insect,
Nesidiocoris tenuis.- Current Progress in Phased Genome Assembly from
Long-Read DNA Sequencing Data.- Laboratory and In-Field Metagenomics for
Environmental Monitoring.- Algorithms for Short-Read Viral Haplotype
Reconstruction: Challenges, Solutions, and Perspectives.- Applying the
Classic Test dN/dS to Detect Selection in Archaea.- Pre-Assembly Read
Processing and Quality Control in Fungal Genomes, Exemplified by the Chytrid
Batrachochytrium salamandrivorans.- Genome Assembly, Polishing, and Analysis
of the Chytrid Batrachochytrium salamandrivorans.- Fusarium Genome Sequencing
and Assembly.- Current Approaches in Genome Assembly of Human Fungal
Pathogens.- A Draft Genome Assembly for the Neglected Tropical Fungal Disease
Pathogen Emarellia grisea and Comparison to Other Pleosporalean Agents
Causing Eumycetoma in Humans.- Seaside to Bedside: Assembly in Research for
Emerging Human Fungal Pathogen Candida auris.- High-Quality Plant Genomes for
and through Pangenomics.- The Cancer Genome in the Long Reads Era.
