This book presents state of the art biophysical approaches to issues in glycobiology that have cutting-edge applications. Despite the importance of glycosylation, the complexity, heterogeneity, and flexibility of the glycans have inhibited their study. Each chapter in this book explains very recent significant advances in biophysical approaches through the use of techniques such as NMR spectroscopy, mass spectrometry, single-molecule imaging, X-ray crystallography, high-speed atomic force microscopy, and computational simulation and their integrative application. Concrete examples are provided of the value of these techniques in addressing key problems in the field. In addition, significant functional glycobiological issues are considered. For example, glycolipids can form dynamic clusters on cell membranes and provide platforms for molecules involved in cell recognition and subsequent signal transduction. The detailed delineation of these molecular systems is discussed, revealing their structural complexity and ability to assemble transiently. This timely book will be of value for graduate students and postdocs interested in frontier topics in glycoscience and also for senior bio-researchers in academic and industrial fields.
Preface.
Chapter 1: The effect of N-glycans on glycoprotein folding and
protein dynamics.
Chapter 2: Synthesis of glycosylated metal complexes for
probing carbohydrate-carbohydrate interactions.
Chapter 3: Unraveling of
lipid raft organization in cell plasma membranes by single-molecule imaging
of ganglioside probes.
Chapter 4: MALDI mass spectrometry imaging of
N-linked glycans in tissues.
Chapter 5: Modern-omics technologies for
isobaric glycan and glycoconjugate structure determination.- Chapter 6:
Synchrotron-radiation vacuum-ultraviolet circular-dichroism spectroscopy for
characterizing the structure of saccharides.
Chapter 7: Biophysical analyses
for probing glycan-protein interactions.
Chapter 8: Structural aspects of ER
glycoprotein quality-control system mediated by glucose tagging.
Chapter 9:
Structural basis of protein Asn-glycosylation by oligosaccharyltransferases.-
Chapter 10: Visualization of functional structure and kinetic dynamics of
cellulases.-Chapter 11: Structure and dynamics of immunoglobulin G
glycoproteins.
Chapter 12: Biophysical Approaches to Solve the Structures of
the Complex Glycan Shield of Chloroviruses.
Chapter 13: Quantifying weak
glycan-protein interactions using a Biolayer Interferometry competition
assay: Applications to ECL lectin and X-31 influenza hemagglutinin.
Yoshiki Yamaguchi, PhD
Synthetic Cellular Chemistry Laboratory
RIKEN Cluster for Pioneering Research
Koichi Kato, PhD Exploratory Research Center on Life and Living Systems National Institutes of Natural Sciences
